We previously demonstrated that octadecylamine-functionalized nanodiamond(ND-ODA)and dexamethasone(Dex)-adsorbed ND-ODA(ND-ODA–Dex)promoted anti-inflammatory and proregenerative behavior in human macrophages in vitro...We previously demonstrated that octadecylamine-functionalized nanodiamond(ND-ODA)and dexamethasone(Dex)-adsorbed ND-ODA(ND-ODA–Dex)promoted anti-inflammatory and proregenerative behavior in human macrophages in vitro.In this study,we performed a pilot study to investigate if these immunomodulatory effects translate when used as a treatment for rheumatoid arthritis in mice.Following local injection in limbs of mice with collagen type II-induced arthritis,microcomputed tomography showed that mice treated with a low dose of ND-ODA and ND-ODA–Dex did not experience bone loss to the levels observed in non-treated arthritic controls.A low dose of ND-ODA and ND-ODA–Dex also reduced macrophage infiltration and expression of proinflammatory mediators iNOS and tumor necrosis factor-a compared to the arthritic control,while a high dose of ND-ODA increased expression of these markers.Overall,these results suggest that ND-ODA may be useful as an inherently immunomodulatory platform,and support the need for an in-depth study,especially with respect to the effects of dose.展开更多
基金the National Institutes of Health grant R01 HL130037.A.P.was supported by Whitaker International and David L.Boren fellowships.
文摘We previously demonstrated that octadecylamine-functionalized nanodiamond(ND-ODA)and dexamethasone(Dex)-adsorbed ND-ODA(ND-ODA–Dex)promoted anti-inflammatory and proregenerative behavior in human macrophages in vitro.In this study,we performed a pilot study to investigate if these immunomodulatory effects translate when used as a treatment for rheumatoid arthritis in mice.Following local injection in limbs of mice with collagen type II-induced arthritis,microcomputed tomography showed that mice treated with a low dose of ND-ODA and ND-ODA–Dex did not experience bone loss to the levels observed in non-treated arthritic controls.A low dose of ND-ODA and ND-ODA–Dex also reduced macrophage infiltration and expression of proinflammatory mediators iNOS and tumor necrosis factor-a compared to the arthritic control,while a high dose of ND-ODA increased expression of these markers.Overall,these results suggest that ND-ODA may be useful as an inherently immunomodulatory platform,and support the need for an in-depth study,especially with respect to the effects of dose.
