Gastro retentive microencapsulated Cefpodoxime Proxetil to improve oral bioavailability

Objective:The objective of the present study was to develop floating microspheres of Cefpodoxime Proxetil in order to achieve an extended retention in the upper GIT,which may result in enhanced absorption and there by improved bioavailability.Methods:The microspheres were prepared by non - aqueous solvent evaporation method using polymers such as Hydroxyl Propyl Methyl Cellulose（HPMC K15M）,Ethyl Cellulose（EC） in different ratios,and Cefpodoxime Proxetil contain in each formulation.In vitro drug release were performed by USP apparatus type I andthe microspheres were characterized by calculating percentage yield,particle size analysis, buoyancy percentage,drug entrapment efficiencyand in vitro drug release studies.Results:The result showed microspheres yield were 50.50%-72.21%,particle size were distributed between75-600μm,drug entrapment efficiency were 14.1%-28.2%,buoyancy percentage were 70.10%-88.25%.Conclusion: Cefpodoxime Proxetil floating microspheres,at the lower polymer to drug ratio,there was a significant drug release. The better drug release profile was seen with FA2 with ratio of drug polymer（1:2）.

Development of a new single dose extended release formulation of cefpodoxime proxetil

Objective:To develop floating microspheres of cefpodoxime proxetil(CP) in order to achieve an extended retention in the upper GIT for 12 hour.Methods:The microspheres were prepared by non aqueous solvent evaporation method using different ratios of cefpodoxime proxetil, hydroxyl propyl methyl cellulose(HPMC K4M ) and ethyl cellulose(1:1:1,1:1:2,1:1:3,1:1:4, 1:1:5 & 1:1:6),in the mixture of dichloromethane and ethanol at ratio of(l:l),with tween80 as the surfactant.Results:The floating microspheres was extended over 10-12 hours and were characterized by particle size analysis(75-600μm),buoyancy percentage(68.1%-85.4%), drug entrapment efficiency(67.5%-88.8%),%yield(50.50%-77.31%) and in vitro drug release was studied for 12 hours.Conclusions:The floating microspheres show better result and it may be use full for prolong the drug release in stomach and improve the bioavailability.

Hepatoprotective effect of Premna corymbosa(Burm.f.) Rottl.& Willd.leaves extract on CCl_4 induced hepatic damage in Wistar albino rats

Objective:To investigate the hepetoprotective activity of Premna corymbosa leaves against carbon tetrachloride（CCl4） induced hepatic damage.Methods:Hepatotoxicity was induced in wistar rats of both sexes by intraperitoneal injection of CCl4,1 mL/kg body weight for every 72 h.The ethanolic extract of Premna corymbosa leaves were administrated at doses of 200 & 400 mL/kg body weight, p.o.,daily for 14 days.The hepatotoxicity and its prevention was assessed by serum markers like serum alkaline phosphatase（SALP）,serum triglycerides（STG）,serum total protein（STP）, serum cholesterol（SC）,and liver wet weight and histopathological studies of the liver.Results:In treatment with the ethanolic extract,the toxic effect of CCl4 was controlled significantly（P【0.01） by restoration of the levels of biochemical parameters as compared to normal and standard drug silymarin treated groups.The liver weight was reduced by the ethanolic extract treated groups. The histopathology of the liver sections evidenced the hepatoprotective activity.Conclusion:The ethanolic extract of the leaves of Premna corymbosa possess significant acute hepatoprotective activity.Premna corymbosa can be recommended for the liver disorders.

Anti-inflammatory activity of Premna corymbosa(Burm.f.) Rottl.& Willd.leaves extracts in Wistar albino rats

Objective:To evaluate the acute toxicity and to investigate the effect of Premna corymbosa ethanolic extract(PCEE) at doses of 200 and 400 mg /kg body weight in acute and chronic models of inflammation in experimental animals.Methods:In the acute toxicity study,a single dose of PCEE of 2 000 mg/kg body weight,p.o.was administered and observed for 48 h.In acute models as egg albumin induced paw edema and chronic model as cotton pellet methods was followed. Results:In acute models,egg albumin induced paw edema PCEE significantly(P【0.01) inhibited the edema formation.In chronic model,cotton pellet induced granuloma formation in rats PCEE significantly(P【0.01) reduced the granuloma formation with percentage inhibition of 35.17%and 50.38%respectively.Conclusions:The present study establishes the antiinflammatory activity of Premna corymbosa leaves.