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铵胁迫下Bacillus aryabhattai NM1-A2脂质代谢的多组学分析
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作者 陆钊梅 kashif muhammad +2 位作者 莫淑名 杜林方 蒋承建 《四川大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第2期178-185,共8页
为了探究脂质代谢在抵抗铵胁迫中的具体作用,本研究以一株高耐铵菌株Bacillus aryabhattai NM1-A2为研究对象,通过全基因组和转录组分析挖掘脂质代谢通路和关键基因,利用实时荧光定量PCR对筛选的差异表达基因进行定量分析,并测定丙二醛... 为了探究脂质代谢在抵抗铵胁迫中的具体作用,本研究以一株高耐铵菌株Bacillus aryabhattai NM1-A2为研究对象,通过全基因组和转录组分析挖掘脂质代谢通路和关键基因,利用实时荧光定量PCR对筛选的差异表达基因进行定量分析,并测定丙二醛含量和抗氧化酶活性.结果表明,铵胁迫下脂肪酸合成相关基因(accC、fabG和fabF等)均上调表达,脂肪酸降解相关基因(ACSL、fadB和fadN等)大多下调表达,可能导致脂肪酸积累.醛脱氢酶编码基因ALDH上调表达以减少脂质氧化产物的积累.此外,铵胁迫诱导超氧化物歧化酶、过氧化物酶和过氧化氢酶表达水平和活性的提高,以减少活性氧产生和细胞损伤.因此,活跃的脂质代谢和抗氧化酶系统是B.aryabhattai NM1-A2抵抗铵胁迫的重要途径. 展开更多
关键词 铵胁迫 脂质代谢 抗氧化 组学分析
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Pore Characteristics of the Fine-Grained Tight Reservoirs in the Yabulai Basin, Northwestern China 被引量:6
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作者 ZHANG Shaomin CAO Yingchang +5 位作者 Jens JAHREN ZHU Rukai MAO Zhiguo XI Kelai kashif muhammad Helge HELLEVANG 《Acta Geologica Sinica(English Edition)》 CAS CSCD 2018年第3期1170-1192,共23页
This work investigated the pore structure characteristics and reservoir features of the finegrained tight reservoirs in the lower member of the Xinhe Formation(J2x1) in the Xiaohu subsag,Yabulai Basin based on core sa... This work investigated the pore structure characteristics and reservoir features of the finegrained tight reservoirs in the lower member of the Xinhe Formation(J2x1) in the Xiaohu subsag,Yabulai Basin based on core samples through various techniques. Interbedded silt/fine sandstones and mudstones are developed in the study area. Scanning electron microscopy(SEM) images were used to delineate different types of pores, including primary intergranular pores, secondary intergranular and intragranular pores, organic pores and fractures. The pore types were distinguished by pore size, pore area, location and formation process. The pore radii of the fine-grained rocks range from 1 nm to 1.55μm, mainly concentrated between 5 and 300 nm by low pressure N2adsorption and MICP analyses. The pore structure parameters of pore throat size and pore throat sorting coefficient are both positively correlated with porosity, while pore throat sorting coefficient has a negative correlation with permeability. The pore structures of the studied samples are much related to the mineral type and content and grain size, followed by TOC content. In these rocks with relatively low TOC and low maturity, the rigid minerals protect pores with pressure shadow from collapse, and dissolution-related pores contribute a lot to inorganic porosity. In contrast, these rocks with abundant TOC contain a large number of organic pores. The permeability of the fine-grained tight reservoir is mainly dominated by larger pore throats, while a large number of small pores(mostly <0.1 μm) contribute considerably to porosity. These results have deepened our understanding of the interbedded fine-grained tight reservoirs and can be applicable to fine-grained reservoirs in a similar setting. 展开更多
关键词 pore characteristics reservoir property fine-grained tight reservoirs Xinhe Formation Yabulai Basin China
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Characteristics and Genesis of the Es_(1) Productive Sandstone Reservoir(Paleogene),Nanpu Sag,East China 被引量:1
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作者 kashif muhammad CAO Yingchang +7 位作者 Ahsan NAVEED Asif muhammad YUAN Guanghui XI Kelai ZHANG Shaomin XU Qisong MUsman TAHIR Isgandarov ORKHAN 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2021年第3期909-927,共19页
The Shahejie Formation is a fundamental rock unit for hydrocarbon exploration and production in the Nanpu Sag,Bohai Bay Basin.Methodology including thin sectioning,core observations,fluorescence,scanning electron micr... The Shahejie Formation is a fundamental rock unit for hydrocarbon exploration and production in the Nanpu Sag,Bohai Bay Basin.Methodology including thin sectioning,core observations,fluorescence,scanning electron microscopy,cathodoluminescence,fluid inclusion,laser scanning confocal microscopy and geochemical analysis(C,O isotopes)were all utilized to investigate the reservoir characteristics and origin of the Es_(1)Sandstone.Thin section study showed that the reservoir rock consisted of feldspathic litharenite and lithic arkose.The reservoir pores are categorized as intergranular pores,fracture pores,dissolution pores and intergranular cement dissolution pores.The studied sandstone had good porosity(0.05%–35%)and permeability(0.006–7000 mD).The Es_(1) reservoir is classified as a fractured reservoir,a primary intergranular pore-associated reservoir and a dissolution reservoir.Deposition,diagenesis and tectogenesis are the main factors that played important roles in the development of the reservoir.Sedimentation is the foundation and assumption for reservoir development,but the effective reservoir is primarily controlled by primary pores,fractures,lithofacies,tectonic elements and dissolution pores.Moreover,compaction,fracture filling and cementation were the primary sources of reservoir densification.The reservoir was progressively formed through the influence of different geological and diagenetic events.The present study provides significant information and references for hydrocarbon exploration and development in the Nanpu Sag. 展开更多
关键词 POROSITY Eocene sandstone Es1 reservoir Nanpu oil field
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