Objective: To achieve maximum post-thaw survival of frozen embryos. Design: Historical controlled study. Setting: Hospital-based fertility center. Patient(s): One hundred forty-five patients whose embryos were frozen ...Objective: To achieve maximum post-thaw survival of frozen embryos. Design: Historical controlled study. Setting: Hospital-based fertility center. Patient(s): One hundred forty-five patients whose embryos were frozen and thawed according to the standard method, and 56 patients whose embryos were frozen and thawed according to a modified method. Intervention( s): Modifications were made to the various steps of cryopreservation: freezing and thawing solutions, loading of embryos into the straws, and warming rates. Main Outcome Measure(s): Post-thaw survival, implantation, and pregnancy rates. Result(s): With the modified method, 138 (93%) of the 149 embryos thawed for 56 patients survived freezing, and 79.8%had all their blastomeres intact, which is almost double the result obtained (41.8%) for patients whose embryos were thawed with the standard method. The implantation and pregnancy rates were also significantly higher with the modified method compared with the standard method. Conclusion(s) : Greater post-thaw embryo survival was achieved, with a concomitant increase in implantation and pregnancy rates, by modifying the various steps in the standard cryopreservation methodology. This has important implications in IVF practice.展开更多
文摘Objective: To achieve maximum post-thaw survival of frozen embryos. Design: Historical controlled study. Setting: Hospital-based fertility center. Patient(s): One hundred forty-five patients whose embryos were frozen and thawed according to the standard method, and 56 patients whose embryos were frozen and thawed according to a modified method. Intervention( s): Modifications were made to the various steps of cryopreservation: freezing and thawing solutions, loading of embryos into the straws, and warming rates. Main Outcome Measure(s): Post-thaw survival, implantation, and pregnancy rates. Result(s): With the modified method, 138 (93%) of the 149 embryos thawed for 56 patients survived freezing, and 79.8%had all their blastomeres intact, which is almost double the result obtained (41.8%) for patients whose embryos were thawed with the standard method. The implantation and pregnancy rates were also significantly higher with the modified method compared with the standard method. Conclusion(s) : Greater post-thaw embryo survival was achieved, with a concomitant increase in implantation and pregnancy rates, by modifying the various steps in the standard cryopreservation methodology. This has important implications in IVF practice.
