Cost-effective phenotyping methods are urgently needed to advance crop genetics in order to meet the food,fuel,and fiber demands of the coming decades.Concretely,characterizing plot level traits in fields is of partic...Cost-effective phenotyping methods are urgently needed to advance crop genetics in order to meet the food,fuel,and fiber demands of the coming decades.Concretely,characterizing plot level traits in fields is of particular interest.Recent developments in highresolution imaging sensors for UAS(unmanned aerial systems)focused on collecting detailed phenotypic measurements are a potential solution.We introduce canopy roughness as a new plant plot-level trait.We tested its usability with soybean by optical data collected from UAS to estimate biomass.We validate canopy roughness on a panel of 108 soybean[Glycine max(L.)Merr.]recombinant inbred lines in a multienvironment trial during the R^(2) growth stage.A senseFly eBee UAS platform obtained aerial images with a senseFly S.O.D.A.compact digital camera.Using a structure from motion(SfM)technique,we reconstructed 3D point clouds of the soybean experiment.A novel pipeline for feature extraction was developed to compute canopy roughness from point clouds.We used regression analysis to correlate canopy roughness with field-measured aboveground biomass(AGB)with a leave-one-out cross-validation.Overall,our models achieved a coefficient of determination(R^(2))greater than 0.5 in all trials.Moreover,we found that canopy roughness has the ability to discern AGB variations among different genotypes.Our test trials demonstrate the potential of canopy roughness as a reliable trait for high-throughput phenotyping to estimate AGB.As such,canopy roughness provides practical information to breeders in order to select phenotypes on the basis of UAS data.展开更多
基金M.H.is funded by the project“Development of Analytical Tools for Drone-Based Canopy Phenotyping in Crop Breeding”from the American Institute of Food and Agriculture(grant number:17000419,WBSE:F.00068834.02.005).E.P.is funded by the project“Upscaling of Carbon Intake and Water Balance Models of Individual Trees to Wider Areas with Short Interval Laser Scanning Time Series”from the Academy of Finland(no.316096).A.B.was in part supported by the NSF CAREER Award No.1845760.
文摘Cost-effective phenotyping methods are urgently needed to advance crop genetics in order to meet the food,fuel,and fiber demands of the coming decades.Concretely,characterizing plot level traits in fields is of particular interest.Recent developments in highresolution imaging sensors for UAS(unmanned aerial systems)focused on collecting detailed phenotypic measurements are a potential solution.We introduce canopy roughness as a new plant plot-level trait.We tested its usability with soybean by optical data collected from UAS to estimate biomass.We validate canopy roughness on a panel of 108 soybean[Glycine max(L.)Merr.]recombinant inbred lines in a multienvironment trial during the R^(2) growth stage.A senseFly eBee UAS platform obtained aerial images with a senseFly S.O.D.A.compact digital camera.Using a structure from motion(SfM)technique,we reconstructed 3D point clouds of the soybean experiment.A novel pipeline for feature extraction was developed to compute canopy roughness from point clouds.We used regression analysis to correlate canopy roughness with field-measured aboveground biomass(AGB)with a leave-one-out cross-validation.Overall,our models achieved a coefficient of determination(R^(2))greater than 0.5 in all trials.Moreover,we found that canopy roughness has the ability to discern AGB variations among different genotypes.Our test trials demonstrate the potential of canopy roughness as a reliable trait for high-throughput phenotyping to estimate AGB.As such,canopy roughness provides practical information to breeders in order to select phenotypes on the basis of UAS data.
