Relationship between catecholamine level and gene polymorphism of β1 adrenergic receptor G1165C in children with EV71 infection in hand foot and mouth disease

Objective:To investigate the relationship between the levels of plasma adrenaline and norepinephrine and gene polymorphism of β1 adrenergic receptor G1165 C in children with enterovirus 71(EV71) infection in hand foot and mouth disease(HFMD). Methods:The polymerase chain reaction(PCR) was used to detect the expression of gene polymorphism of β1 adrenergic receptor G1165 C in vitro. The levels of plasma adrenaline and norepinephrine were measured by enzyme-linked immunosorbent assay(ELISA). Results:The plasma norepinephrine level of severe group was significantly higher than the mild group in children with EV71 infection in HFMD(P<0.05); however,the levels of plasma adrenalinein in two groups had no statistical differences(P>0.05); There was no significant difference in the distribution of β1 adrenergic receptor G1165 C genotype and allele between EV71 infection group and healthy control group(P> 0.05). Further analysis of EV71 infection group by dividing it into mild and severe groups showed that there was no significant difference in the distribution of genotype and allele between these two groups as well(P> 0.05). There was no significant difference in the levels of epinephrine and norepinephrine in different genotypes of EV71 infection group(P> 0.05),and in the levels of plasma epinephrine and norepinephrine in the mild and severe groups(P> 0.05). Conclusions:As the disease gets worse,the plasma norepinephrine level has a rising trend in children with EV71 infection in HFMD,which is an important indicator to evaluate the progress of the disease. However,the gene polymorphism of eptor G1165 C have no significant correlation,not only with the susceptibility and severit β1 adrenergic recy of EV71 infection in hand,foot and mouth disease,but also with the levels of catecholamine.

原发性衰老相关性tau病在中国人群中的临床病理学研究

目的:分析中国人群中原发性衰老相关性tau病(PART)的临床病理学特点,并比较其与老年性痴呆之间的差异。创新点:PART为新近提出的概念,其与老年性痴呆的关系尚存在争论。目前未见针对中国人群PART的相关报道。本文首次报道了浙江大学医学院中国人脑库中的PART的临床病理学特点。方法:回顾性分析相关病例,严格纳入标准。PART在中国人群中常见,50岁以上人群占47%。平均年龄(76±12)岁,女性14例(30%),男性32例(70%)。免疫组织化学法显示,按磷酸化tau的Braak分期,PART大多为I到Ш期。异常蛋白TDP43和p62在PART中高表达,分别为67%和70%;在老年性痴呆中分别为83%和83%。TDP43和p62共表达在PART为55%,在老年性痴呆为79%。其中海马为PART常见累及部位,TDP43为61%,p62为63%。仅4%的PART患者表现为轻度认知障碍。结论:PART在中国人群中常见;PART大多无明显痴呆症状;异常蛋白TDP43和p62在PART中高表达,与老年性痴呆相比存在差异。