AIM:To study the effect of staurosporine (ST) on the cell cycle of human gastric cancer cell lines MGC803 and SGC7901.METHODS:Cell proliferation was evaluated by trypan blue dye exclusion method.Apoptotic morphology w...AIM:To study the effect of staurosporine (ST) on the cell cycle of human gastric cancer cell lines MGC803 and SGC7901.METHODS:Cell proliferation was evaluated by trypan blue dye exclusion method.Apoptotic morphology was observed under a transmission electron microscope.Changes of cell cycle and apoptotic peaks of cells were determined by flow cytometry. Expression of p21^WAF1 gene was examined using immunohistochemistry and RT-PCR.RESULTS:The growth of MGC803 and SGC7901 cells was inhibited by ST.The inhibitory concentrations against 50% cells (IC50) at 24 h and 48 h were 54ng/ml and 23ng/ml for MGC803, and 61ng/ml and 37ng/ml for SGC7901.Typical apoptotic bodies and apoptotic peaks were observed 24h after cells were treated wth ST at a concentration of 200ng/ml.The percentage of cells at G0/G1 phase was decreased and that of cells at G2/M was increased significantly in the group treated wth ST at the concentrations of 40ng/ml,60ng/ml,100ng/ml for 24h,compared with the control group (P<0.01).The expression levels of p21^WAF1 gene in both MGC803 and SGC7901 cells were markedly up-regulated after treatment with ST.CONCLUSION:ST can cause arrest of gastric cancer cells at G2/M phase,which may be one of the mechanisms that inhibit cell proliferation and cause apoptosis in these cells.Effect of ST on cells at G2/M phase may be attributed to the up-regulattion of p21^WAF1 gene.展开更多
基金Supported bYThe China State Key Basic Research Program,No.G1998051203
文摘AIM:To study the effect of staurosporine (ST) on the cell cycle of human gastric cancer cell lines MGC803 and SGC7901.METHODS:Cell proliferation was evaluated by trypan blue dye exclusion method.Apoptotic morphology was observed under a transmission electron microscope.Changes of cell cycle and apoptotic peaks of cells were determined by flow cytometry. Expression of p21^WAF1 gene was examined using immunohistochemistry and RT-PCR.RESULTS:The growth of MGC803 and SGC7901 cells was inhibited by ST.The inhibitory concentrations against 50% cells (IC50) at 24 h and 48 h were 54ng/ml and 23ng/ml for MGC803, and 61ng/ml and 37ng/ml for SGC7901.Typical apoptotic bodies and apoptotic peaks were observed 24h after cells were treated wth ST at a concentration of 200ng/ml.The percentage of cells at G0/G1 phase was decreased and that of cells at G2/M was increased significantly in the group treated wth ST at the concentrations of 40ng/ml,60ng/ml,100ng/ml for 24h,compared with the control group (P<0.01).The expression levels of p21^WAF1 gene in both MGC803 and SGC7901 cells were markedly up-regulated after treatment with ST.CONCLUSION:ST can cause arrest of gastric cancer cells at G2/M phase,which may be one of the mechanisms that inhibit cell proliferation and cause apoptosis in these cells.Effect of ST on cells at G2/M phase may be attributed to the up-regulattion of p21^WAF1 gene.
