lon gene,which belongs to a family of heat shock genes,has been found in manykinds of bacteria and animals.Its peptide product is defined as La-protein,which is anATP-dependent serine-proteinase.It always works as hea...lon gene,which belongs to a family of heat shock genes,has been found in manykinds of bacteria and animals.Its peptide product is defined as La-protein,which is anATP-dependent serine-proteinase.It always works as heat shock protein.The study on longene only started in the 1980s.During the past decade,great progress has been achievedon the study of the structure,function and regulation of La.The lon gene has been foundin E.coli,Bacillus,Myxococous xanthus,Erwinia amylovora,etc.lon is also foundto be indispensable for vegetative growth in M.xanthus.The strain cannot grow展开更多
The promoters, PTH4 and P-TH270 involved in the regulation of Streptomyces coelicolor differentiation were subcloned into Streptomyces promoter, i.e. probe plasmid pIJ4083, and the recombinant plasmids, pIJ4470 and pI...The promoters, PTH4 and P-TH270 involved in the regulation of Streptomyces coelicolor differentiation were subcloned into Streptomyces promoter, i.e. probe plasmid pIJ4083, and the recombinant plasmids, pIJ4470 and pIJ4471, were constructed. Two promoters could drive the expression of reporter gene encoding catechol dioxygenase when pIJ4470 and pIJ4471 were introduced into some white mutants (C85, C70, C71, C17 and C119). The total RNA was isolated from these strains containing recombinant plasmid. Probes were prepared by labelling 5 -ends of PTH4 AND PTH270 DNA fragments using radioisotope. DNA - RNA hybridization was carried out with the probes and RNAs isolated from different strains. The S1 mapping result showed that all RNAs from strains of C85/pIJ4470, C85/4471, C70/pIJ4470, C70/pIJ4471 and C17/pIJ4470 as well as C17/pIJ4471 gave rise to strong positive hy-bridization signal, whereas RNAs from C71/pIJ4470 and C71/pIJ4471 did not give any positive signal. RNAs from C119/pIJ4470 and C119/pIJ4471 gave weak hybridization signal. The result indicated that the transcription of both PTH4 and PTH270 promoters might depend on whiG, an essential gene in Streptomyces differentiation, and partially de-pend on whiH, but they did not depend on other differentiation genes (whiA, whiB and whiI) .展开更多
基金supported by Chinese Academy of Sciences and the Royal Society of UK.
文摘lon gene,which belongs to a family of heat shock genes,has been found in manykinds of bacteria and animals.Its peptide product is defined as La-protein,which is anATP-dependent serine-proteinase.It always works as heat shock protein.The study on longene only started in the 1980s.During the past decade,great progress has been achievedon the study of the structure,function and regulation of La.The lon gene has been foundin E.coli,Bacillus,Myxococous xanthus,Erwinia amylovora,etc.lon is also foundto be indispensable for vegetative growth in M.xanthus.The strain cannot grow
基金Proiect supported by the Chinese Academy of Sciences and the Royal Society of UK.
文摘The promoters, PTH4 and P-TH270 involved in the regulation of Streptomyces coelicolor differentiation were subcloned into Streptomyces promoter, i.e. probe plasmid pIJ4083, and the recombinant plasmids, pIJ4470 and pIJ4471, were constructed. Two promoters could drive the expression of reporter gene encoding catechol dioxygenase when pIJ4470 and pIJ4471 were introduced into some white mutants (C85, C70, C71, C17 and C119). The total RNA was isolated from these strains containing recombinant plasmid. Probes were prepared by labelling 5 -ends of PTH4 AND PTH270 DNA fragments using radioisotope. DNA - RNA hybridization was carried out with the probes and RNAs isolated from different strains. The S1 mapping result showed that all RNAs from strains of C85/pIJ4470, C85/4471, C70/pIJ4470, C70/pIJ4471 and C17/pIJ4470 as well as C17/pIJ4471 gave rise to strong positive hy-bridization signal, whereas RNAs from C71/pIJ4470 and C71/pIJ4471 did not give any positive signal. RNAs from C119/pIJ4470 and C119/pIJ4471 gave weak hybridization signal. The result indicated that the transcription of both PTH4 and PTH270 promoters might depend on whiG, an essential gene in Streptomyces differentiation, and partially de-pend on whiH, but they did not depend on other differentiation genes (whiA, whiB and whiI) .
