Global carbon cycle has received extensive attention,among which the river-estuary system is one of the important links connecting the carbon cycle between land and ocean.In this paper,the distribution and control fac...Global carbon cycle has received extensive attention,among which the river-estuary system is one of the important links connecting the carbon cycle between land and ocean.In this paper,the distribution and control factors of particulate organic carbon(POC)were studied by using the data of organic carbon contents and its carbon isotopic composition(δ13C)in the mainstream and estuary of Passur River in the Sundarbans area,combined with the hydrological and biological data measured by CTD.The results show that POC content ranged from 0.263 mg/L to 9.292 mg/L,and the POC content in the river section(averaged 4.129 mg/L)was significantly higher than that in the estuary area(averaged 0.858 mg/L).Two distinct stages of POC transport from land to sea in the Sundarbans area were identified.The first stage occurred in the river section,where POC distribution was mainly controlled by the dynamic process of runoff and the organic carbon was mainly terrestrial source.The second stage occurred during estuarine mixing,where the POC distribution was mainly controlled by the mixing process of seawater and freshwater.The source of POC was predominantly marine and exhibiting vertical differences.The surface and middle layers were primarily influenced by marine sources,while the bottom layer was jointly controlled by terrestrial and marine sources of organic carbon.These findings are of great significance for understanding the carbon cycle in such a large mangrove ecosystem like the Sundarbans mangrove.展开更多
Neuropathic pain is a chronic disease that severely afflicts the life and emotional status of patients,but currently available treatments are often ineffective.Novel therapeutic targets for the alleviation of neuropat...Neuropathic pain is a chronic disease that severely afflicts the life and emotional status of patients,but currently available treatments are often ineffective.Novel therapeutic targets for the alleviation of neuropathic pain are urgently needed.Rhodojaponin Ⅵ,a grayanotoxin from Rhododendron molle,showed remarkable antinociceptive efficacy in models of neuropathic pain,but its biotargets and mechanisms are unknown.Given the reversible action of rhodojaponin Ⅵ and the narrow range over which its structure can be modified,we perforwmed thermal proteome profiling of the rat dorsal root ganglion to determine the protein target of rhodojaponin Ⅵ.N-Ethylmaleimide-sensitive fusion(NSF) was confirmed as the key target of rhodojaponin Ⅵ through biological and biophysical experiments.Functional validation showed for the first time that NSF facilitated trafficking of the Cav2.2 channel to induce an increase in Ca2+current intensity,whereas rhodojaponin Ⅵ reversed the effects of NSF.In conclusion,rhodojaponin Ⅵ represents a unique class of analgesic natural products targeting Cav2.2 channels via NSF.展开更多
基金The Scientific Research Foundation of the Third Institute of Oceanography,Ministry of Natural Resources under contract Nos TIO2020008 and TIO2019028the Project of Marine Protected Areas Network in China-ASEAN Countries,National Key Research and Development Programe under contract No.2017YFC1405100the National Science Foundation of China under contract No.41976050.
文摘Global carbon cycle has received extensive attention,among which the river-estuary system is one of the important links connecting the carbon cycle between land and ocean.In this paper,the distribution and control factors of particulate organic carbon(POC)were studied by using the data of organic carbon contents and its carbon isotopic composition(δ13C)in the mainstream and estuary of Passur River in the Sundarbans area,combined with the hydrological and biological data measured by CTD.The results show that POC content ranged from 0.263 mg/L to 9.292 mg/L,and the POC content in the river section(averaged 4.129 mg/L)was significantly higher than that in the estuary area(averaged 0.858 mg/L).Two distinct stages of POC transport from land to sea in the Sundarbans area were identified.The first stage occurred in the river section,where POC distribution was mainly controlled by the dynamic process of runoff and the organic carbon was mainly terrestrial source.The second stage occurred during estuarine mixing,where the POC distribution was mainly controlled by the mixing process of seawater and freshwater.The source of POC was predominantly marine and exhibiting vertical differences.The surface and middle layers were primarily influenced by marine sources,while the bottom layer was jointly controlled by terrestrial and marine sources of organic carbon.These findings are of great significance for understanding the carbon cycle in such a large mangrove ecosystem like the Sundarbans mangrove.
基金The Natural Science Foundation of China (Nos.21732008,and 81771205)CAMS Innovation Fund for Medical Sciences (CIFMS,Nos.CIFMS-2022-I2M-JB-009,China)The National Postdoctoral Program for Innovative Talents (No.BX20180053,China)
文摘Neuropathic pain is a chronic disease that severely afflicts the life and emotional status of patients,but currently available treatments are often ineffective.Novel therapeutic targets for the alleviation of neuropathic pain are urgently needed.Rhodojaponin Ⅵ,a grayanotoxin from Rhododendron molle,showed remarkable antinociceptive efficacy in models of neuropathic pain,but its biotargets and mechanisms are unknown.Given the reversible action of rhodojaponin Ⅵ and the narrow range over which its structure can be modified,we perforwmed thermal proteome profiling of the rat dorsal root ganglion to determine the protein target of rhodojaponin Ⅵ.N-Ethylmaleimide-sensitive fusion(NSF) was confirmed as the key target of rhodojaponin Ⅵ through biological and biophysical experiments.Functional validation showed for the first time that NSF facilitated trafficking of the Cav2.2 channel to induce an increase in Ca2+current intensity,whereas rhodojaponin Ⅵ reversed the effects of NSF.In conclusion,rhodojaponin Ⅵ represents a unique class of analgesic natural products targeting Cav2.2 channels via NSF.
