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Elucidation of the MicroRNA Transcriptome in Western Corn Rootworm Reveals Its Dynamic and Evolutionary Complexity
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作者 Xiaozeng Yang Elane Fishilevich +6 位作者 Marcelo A.German Premchand Gandra Robert E.McEwan André Billion Eileen Knorr Andreas Vilcinskas kenneth e.narva 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2021年第5期800-814,共15页
Diabrotica virgifera virgifera(western corn rootworm,WCR)is one of the most destructive agricultural insect pests in North America.It is highly adaptive to environmental stimuli and crop protection technologies.Howeve... Diabrotica virgifera virgifera(western corn rootworm,WCR)is one of the most destructive agricultural insect pests in North America.It is highly adaptive to environmental stimuli and crop protection technologies.However,little is known about the underlying genetic basis of WCR behavior and adaptation.More specifically,the involvement of small RNAs(s RNAs),especially micro RNAs(mi RNAs),a class of endogenous small non-coding RNAs that regulate various biological processes,has not been examined,and the datasets of putative s RNA sequences have not previously been generated for WCR.To achieve a comprehensive collection of s RNA transcriptomes in WCR,we constructed,sequenced,and analyzed s RNA libraries from different life stages of WCR and northern corn rootworm(NCR),and identified 101 conserved precursor mi RNAs(pre-mi RNAs)in WCR and other Arthropoda.We also identified 277 corn rootworm specific pre-mi RNAs.Systematic analyses of s RNA populations in WCR revealed that its s RNA transcriptome,which includes PIWI-interacting RNAs(pi RNAs)and mi RNAs,undergoes a dynamic change throughout insect development.Phylogenetic analysis of mi RNA datasets from model species reveals that a large pool of species-specific mi RNAs exists in corn rootworm;these are potentially evolutionarily transient.Comparisons of WCR mi RNA clusters to other insect species highlight conserved mi RNA-regulated processes that are common to insects.Parallel Analysis of RNA Ends(PARE)also uncovered potential mi RNA-guided cleavage sites in WCR.Overall,this study provides a new resource for studying the s RNA transcriptome and mi RNA-mediated gene regulation in WCR and other Coleopteran insects. 展开更多
关键词 Western corn rootworm Small RNA MICRORNA Parallel Analysis of RNA Ends PIWI-interacting RNA
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