The giant freshwater prawn(Macrobrachium rosenbergii)is a commercially cultured species in China.A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes.Here,based on the ...The giant freshwater prawn(Macrobrachium rosenbergii)is a commercially cultured species in China.A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes.Here,based on the results of reduced-representation genome sequencing and genome survey of M.rosenbergii,we identified steady sex-linked markers.Further nucleotide alignment analysis revealed 83.42%nucleotide similarity between the amplified W-and Z-chromosome fragments.qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females.Similarly,the W chromosome-specific fragments were present in super females approximately twice as often compared to females.Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes of M.rosenbergii.展开更多
Grass carp has the highest production yield among all the farmed fish species around the world.But the molecular and genetic basis of growth traits is inadequately understood in this fish.In the present study,we apply...Grass carp has the highest production yield among all the farmed fish species around the world.But the molecular and genetic basis of growth traits is inadequately understood in this fish.In the present study,we apply whole-transcriptome sequencing to identify genes whose expression is different between fast and slow growing grass carp.We identified 1178 differentially expressed genes(DEGs),among which 270 were upregulated in the fast growing group and 908 upregulated in the slow growing group.The GO enrichment analysis revealed that DEGs was significantly enriched in protein synthesis and export machinery,cell division and development and metabolism,suggesting that cell division and metabolism may play key roles in development processes of fish.KEGG pathway analysis showed that MAPK signaling pathway may play a prominent role in growth difference.In addition,the majority of genes in immune responses related pathway were upregulated in the slow growing individuals.These results provide some valuable resources for understanding the molecular mechanisms of growth traits in fish.展开更多
基金This work was supported by the National Key R&D Program of China(project number 2018YFD0900201)the Natural Science Foundation of China(project number 31772841 and 31902348)the Shanghai Sailing Program(project number 17YF1408000).
文摘The giant freshwater prawn(Macrobrachium rosenbergii)is a commercially cultured species in China.A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes.Here,based on the results of reduced-representation genome sequencing and genome survey of M.rosenbergii,we identified steady sex-linked markers.Further nucleotide alignment analysis revealed 83.42%nucleotide similarity between the amplified W-and Z-chromosome fragments.qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females.Similarly,the W chromosome-specific fragments were present in super females approximately twice as often compared to females.Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes of M.rosenbergii.
基金the Project of Shanghai Engineering and Technology Center for Promoting Ability(19DZ2284300)the Earmarked Fund for the China Agriculture Research System(CARS-45-03)。
文摘Grass carp has the highest production yield among all the farmed fish species around the world.But the molecular and genetic basis of growth traits is inadequately understood in this fish.In the present study,we apply whole-transcriptome sequencing to identify genes whose expression is different between fast and slow growing grass carp.We identified 1178 differentially expressed genes(DEGs),among which 270 were upregulated in the fast growing group and 908 upregulated in the slow growing group.The GO enrichment analysis revealed that DEGs was significantly enriched in protein synthesis and export machinery,cell division and development and metabolism,suggesting that cell division and metabolism may play key roles in development processes of fish.KEGG pathway analysis showed that MAPK signaling pathway may play a prominent role in growth difference.In addition,the majority of genes in immune responses related pathway were upregulated in the slow growing individuals.These results provide some valuable resources for understanding the molecular mechanisms of growth traits in fish.
