Objective:To determine the molecular epidemiology of extended-spectrumβ-lactamase （ESRL） by testing a cohort of clinical ESBL-producing bacterial isolates that were isolated in the Kingdom of Bahrain.Methods:ESBL p...Objective:To determine the molecular epidemiology of extended-spectrumβ-lactamase （ESRL） by testing a cohort of clinical ESBL-producing bacterial isolates that were isolated in the Kingdom of Bahrain.Methods:ESBL producing Enterabacleriaceae isolates（based on phenotypie tests） were collected from Microbiology Laboratory ot the Salmaniya Medical Complex,Bahrain between January-June 2006.Antibiotic susceptibility to a panel of antibiotics was performed and bla<sub>CTX-M</sub> genes were detected by multiplex PCR.Results:A total of 230 isolates（Escherichia coli.n=180:Klebsiella pneumoniae,n=50） were studied.98%were CTX-M type.For Escherichia coli isolates.65（36.1%） harbored CTXM+TF.M combination and 68（37.8%） had CTX-M alone.In contrast,for Klebsiella pneumoniae isolates only 5（10.0%） harbored the CTX-M combination,and none had CTX-M only.The bla<sub>CTX-M</sub> gene was found predominantly in urine isolates（n=145/230;63.0%）.Sensitivity to imipenem and nitrofurantoin was 100%and 60%.respectively.CTX-M carriage was associated with the resistance lo fluoroquinolones, trimethoprim-sulfamethoxazole and aminoglycosides.Conclusions:Our study documentes high prevalence of CTX-M ESBL type among Escherichia call and Klebsiella from the Kingdom of Bahrain.The apparent dissemination of CTX-M producers could represent a substantial barrier in the treatment of community-acquired infections.The use of extended-Spectrum cephalosporins. quinolones,and aminoglycosides is compromised,leaving carbapenems as the therapeutic option for severe infections caused by ESBL producers.展开更多
文摘Objective:To determine the molecular epidemiology of extended-spectrumβ-lactamase （ESRL） by testing a cohort of clinical ESBL-producing bacterial isolates that were isolated in the Kingdom of Bahrain.Methods:ESBL producing Enterabacleriaceae isolates（based on phenotypie tests） were collected from Microbiology Laboratory ot the Salmaniya Medical Complex,Bahrain between January-June 2006.Antibiotic susceptibility to a panel of antibiotics was performed and bla<sub>CTX-M</sub> genes were detected by multiplex PCR.Results:A total of 230 isolates（Escherichia coli.n=180:Klebsiella pneumoniae,n=50） were studied.98%were CTX-M type.For Escherichia coli isolates.65（36.1%） harbored CTXM+TF.M combination and 68（37.8%） had CTX-M alone.In contrast,for Klebsiella pneumoniae isolates only 5（10.0%） harbored the CTX-M combination,and none had CTX-M only.The bla<sub>CTX-M</sub> gene was found predominantly in urine isolates（n=145/230;63.0%）.Sensitivity to imipenem and nitrofurantoin was 100%and 60%.respectively.CTX-M carriage was associated with the resistance lo fluoroquinolones, trimethoprim-sulfamethoxazole and aminoglycosides.Conclusions:Our study documentes high prevalence of CTX-M ESBL type among Escherichia call and Klebsiella from the Kingdom of Bahrain.The apparent dissemination of CTX-M producers could represent a substantial barrier in the treatment of community-acquired infections.The use of extended-Spectrum cephalosporins. quinolones,and aminoglycosides is compromised,leaving carbapenems as the therapeutic option for severe infections caused by ESBL producers.
