Objective:To investigate the larvicidai activity of essential oil and methanol extract of the Nepeta menthoides（N.menthoides） against main malaria vector,Anopheles stephensi（An. stephensi）.Methods:The essential oi...Objective:To investigate the larvicidai activity of essential oil and methanol extract of the Nepeta menthoides（N.menthoides） against main malaria vector,Anopheles stephensi（An. stephensi）.Methods:The essential oil of planl was obtained by Clevenger type apparatus and the methanol extract was supplied with Percolation method,larvicidai activity was tested by WHO method.Twenty five fourth-instar larvae of An.stephensi were used in the larvicidai assay and four replicates were tested for each concentration.Five different concentrations of the oil and extract were tested for calculation of LC<sub>50</sub> and LC<sub>50</sub> values.Results:The LC<sub>50</sub> and LC<sub>90</sub> values were determined by probit analysis.IC<sub>50</sub> was 69.5 and 234.3 ppm and LC<sub>50</sub> was 175.5 and 419.9 ppm for the extract and essential oil respectively.Conclusions:According to the results of this study methanolic extract of plant exhibited more larvicidai activity than essential oil.This could be useful for investigation of new natural larvicidai compounds.展开更多
基金financially supported by Tehran University of Medical Sciences
文摘Objective:To investigate the larvicidai activity of essential oil and methanol extract of the Nepeta menthoides（N.menthoides） against main malaria vector,Anopheles stephensi（An. stephensi）.Methods:The essential oil of planl was obtained by Clevenger type apparatus and the methanol extract was supplied with Percolation method,larvicidai activity was tested by WHO method.Twenty five fourth-instar larvae of An.stephensi were used in the larvicidai assay and four replicates were tested for each concentration.Five different concentrations of the oil and extract were tested for calculation of LC<sub>50</sub> and LC<sub>50</sub> values.Results:The LC<sub>50</sub> and LC<sub>90</sub> values were determined by probit analysis.IC<sub>50</sub> was 69.5 and 234.3 ppm and LC<sub>50</sub> was 175.5 and 419.9 ppm for the extract and essential oil respectively.Conclusions:According to the results of this study methanolic extract of plant exhibited more larvicidai activity than essential oil.This could be useful for investigation of new natural larvicidai compounds.
