Objective:To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL pathogenesis among Iranian patients.Methods:Three groups including patients with VL clinical presentation and leish...Objective:To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL pathogenesis among Iranian patients.Methods:Three groups including patients with VL clinical presentation and leishmania seropositive(n=124).patients seropositive but without clinical presentation(n=82) and healthy controls(n=63) were selected to conduct this cross-sectional study.Polymorphism at +2767 position of IL-8 was investigated using PCR-RPLP techniques.Anti-leishmania antibody titration was evaluated by the immunoflorescence technique.Results:We observed higher significant frequencies +2767 A/A and A/T genotypes in groups I compared to group 2 and healthy controls(P=0.001).Also,patients in Group 1 carriyng A/A genotype showed higher liter of antileshmania antibody than patients with A/T and T/T genotypes(P=0.05).The validity of the data was analyzed using Hardy-Weinberg equilibrium and one way analysis of variance(ANOVA),as well as x^2tests.Conclusions:Our findings indicate that the IL-8 +2767 polymorphism is significantly involved in impaired immune responses against VL and it could be considered as a risk factor for the VL progress.展开更多
基金supported by a grant from the Tabriz University of Medical Sciences(No.91-20)
文摘Objective:To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL pathogenesis among Iranian patients.Methods:Three groups including patients with VL clinical presentation and leishmania seropositive(n=124).patients seropositive but without clinical presentation(n=82) and healthy controls(n=63) were selected to conduct this cross-sectional study.Polymorphism at +2767 position of IL-8 was investigated using PCR-RPLP techniques.Anti-leishmania antibody titration was evaluated by the immunoflorescence technique.Results:We observed higher significant frequencies +2767 A/A and A/T genotypes in groups I compared to group 2 and healthy controls(P=0.001).Also,patients in Group 1 carriyng A/A genotype showed higher liter of antileshmania antibody than patients with A/T and T/T genotypes(P=0.05).The validity of the data was analyzed using Hardy-Weinberg equilibrium and one way analysis of variance(ANOVA),as well as x^2tests.Conclusions:Our findings indicate that the IL-8 +2767 polymorphism is significantly involved in impaired immune responses against VL and it could be considered as a risk factor for the VL progress.
