2018年,世界各地都将展现前所未有的综合经济发展实力。由Alpha One Capital Partners开展的研究表明,在2017年,经济合作与发展组织（OECD）追踪的所有45个国家/地区的经济自2007年以来均实现了首次增长,其中33个国家/地区的经济在2017...2018年,世界各地都将展现前所未有的综合经济发展实力。由Alpha One Capital Partners开展的研究表明,在2017年,经济合作与发展组织（OECD）追踪的所有45个国家/地区的经济自2007年以来均实现了首次增长,其中33个国家/地区的经济在2017年经历了加速增长。例如,美国国内生产总值从2016年的1.5%加速增长到2017年的2.2%,而且2018年预计将达到2.5%。由于面向全球市场,展开更多
Objective:The aim of this work was to explore the feasibility of in vivo and non-invasive monitoring of deuterium/hydrogen(^(2)H/1H)exchange at the metabolic level upon exposure to heavy water(^(2)H_(2)O).Methods:The ...Objective:The aim of this work was to explore the feasibility of in vivo and non-invasive monitoring of deuterium/hydrogen(^(2)H/1H)exchange at the metabolic level upon exposure to heavy water(^(2)H_(2)O).Methods:The healthy female mice were randomly assigned to two groups after day 0 when both mice received standard drinking water.The treated mouse was fed with^(2)H_(2)O(80%,v/v)and the control mouse fed with standard drinking water(H2O)over next 13days.Real-time mass spectrometric analysis of volatile metabolism emitted through breathing and the skin was performed on days 1,2,3,10,12,and 13.Animal experiment was approved by the Laboratory Animal Ethics Committee of Jinan University(approval No.20161117163322)on October 29,2021.Results:We observed a replacement of 1H by^(2)H in 52 mass spectral features(60^(2)H/1H isotopologue pairs)for the mouse fed with^(2)H_(2)O,but not for the control mouse.These included pyruvic acid and lactic acid,lysine and methyl-lysine as well as short-chain fatty acids comprising acetic acid,propionic acid,butyric acid and valeric acid.Conclusion:Secondary electrospray ionization-high resolution mass spectrometry allows monitoring in vivo^(2)H-incorporation of metabolites in a non-invasive and real-time setup and opens new opportunities to use^(2)H tracing to extend current metabolic studies,especially those with a focus on anaerobic glycolysis,lysine methylation and gut microbiome via monitoring of short-chain fatty acids.展开更多
文摘2018年,世界各地都将展现前所未有的综合经济发展实力。由Alpha One Capital Partners开展的研究表明,在2017年,经济合作与发展组织（OECD）追踪的所有45个国家/地区的经济自2007年以来均实现了首次增长,其中33个国家/地区的经济在2017年经历了加速增长。例如,美国国内生产总值从2016年的1.5%加速增长到2017年的2.2%,而且2018年预计将达到2.5%。由于面向全球市场,
基金This work was supported by a grant from Fondation Botnar(Switzerland)and the Swiss National Science Foundation(Nos.320030_173168 and PCEGP3_181300,to PS)the National Natural Science Foundation of China(No.22122603,to XL)+1 种基金Guangdong Special Support Program(No.2019BT02Z546,to XL)Guangdong Natural Science Foundation(Nos.2018A030313790 and 2017GC010648,to HZ).
文摘Objective:The aim of this work was to explore the feasibility of in vivo and non-invasive monitoring of deuterium/hydrogen(^(2)H/1H)exchange at the metabolic level upon exposure to heavy water(^(2)H_(2)O).Methods:The healthy female mice were randomly assigned to two groups after day 0 when both mice received standard drinking water.The treated mouse was fed with^(2)H_(2)O(80%,v/v)and the control mouse fed with standard drinking water(H2O)over next 13days.Real-time mass spectrometric analysis of volatile metabolism emitted through breathing and the skin was performed on days 1,2,3,10,12,and 13.Animal experiment was approved by the Laboratory Animal Ethics Committee of Jinan University(approval No.20161117163322)on October 29,2021.Results:We observed a replacement of 1H by^(2)H in 52 mass spectral features(60^(2)H/1H isotopologue pairs)for the mouse fed with^(2)H_(2)O,but not for the control mouse.These included pyruvic acid and lactic acid,lysine and methyl-lysine as well as short-chain fatty acids comprising acetic acid,propionic acid,butyric acid and valeric acid.Conclusion:Secondary electrospray ionization-high resolution mass spectrometry allows monitoring in vivo^(2)H-incorporation of metabolites in a non-invasive and real-time setup and opens new opportunities to use^(2)H tracing to extend current metabolic studies,especially those with a focus on anaerobic glycolysis,lysine methylation and gut microbiome via monitoring of short-chain fatty acids.
