Background: Immunoglobulins have immune-modulating capacities and are used for the treatment of different dermatological diseases. They have also been reported for the treatment of severe atopic dermatitis (AD). Objec...Background: Immunoglobulins have immune-modulating capacities and are used for the treatment of different dermatological diseases. They have also been reported for the treatment of severe atopic dermatitis (AD). Objectives: To determine the effects of immunoglobulins on the phenotype and function of peripheral T and B lymphocytes from patients with AD in comparison with healthy donors (HD) as controls. Methods: We studied lymphocyte activation and T-cell cytokine production from 12 patients with AD and 10 HD by multicolour flow cytometric analysis in the presence of immunoglobulins. Results: Immunoglobulins significantly inhibited T-cell activation (CD69), by 71%(AD) and by 62%(HD). Production of interferon-γand interleukin-4 was also significantly inhibited, by 44%/24%(AD) and 38%/10%(HD), respectively. In addition, CD86 expression on B lymphocytes was downregulated by 30%in AD and by 29%in HD, whereas CD23 expression was decreased without reaching statistical significance. Conclusions: Our data demonstrate that, in vitro, immunoglobulins modulate the activation and cytokine production of peripheral blood lymphocytes from both HD and patients with AD.展开更多
文摘Background: Immunoglobulins have immune-modulating capacities and are used for the treatment of different dermatological diseases. They have also been reported for the treatment of severe atopic dermatitis (AD). Objectives: To determine the effects of immunoglobulins on the phenotype and function of peripheral T and B lymphocytes from patients with AD in comparison with healthy donors (HD) as controls. Methods: We studied lymphocyte activation and T-cell cytokine production from 12 patients with AD and 10 HD by multicolour flow cytometric analysis in the presence of immunoglobulins. Results: Immunoglobulins significantly inhibited T-cell activation (CD69), by 71%(AD) and by 62%(HD). Production of interferon-γand interleukin-4 was also significantly inhibited, by 44%/24%(AD) and 38%/10%(HD), respectively. In addition, CD86 expression on B lymphocytes was downregulated by 30%in AD and by 29%in HD, whereas CD23 expression was decreased without reaching statistical significance. Conclusions: Our data demonstrate that, in vitro, immunoglobulins modulate the activation and cytokine production of peripheral blood lymphocytes from both HD and patients with AD.
