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Quantifying and sorting of gold nanoparticle dimers from complex reaction mixtures using flow cytometry
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作者 Jens B. Simonsen Nini E. a. Reeler +3 位作者 anna Fossum knud a. lerstrup Bo W. Laursen Kasper Nergaard 《Nano Research》 SCIE EI CAS CSCD 2016年第10期3093-3098,共6页
The formation, characterization, and purification of well-defined stoichiometric clusters of metallic nanoparticles, particularly in the form of dimers or trimers, are important and formidable challenges in nanoscienc... The formation, characterization, and purification of well-defined stoichiometric clusters of metallic nanoparticles, particularly in the form of dimers or trimers, are important and formidable challenges in nanoscience. Here we show that flow cytometry can be used as a high-throughput method to determine the relative distribution of oligomeric clusters of molecularly linked gold nano- particles in bulk solution at the single-particle level with good statistics. This unique information would be near impossible to obtain using traditional characterization techniques. The flow cytometric approach is utilized to provide fast feedback for the synthesis optimization of the complex reaction between citrate-stabilized gold nanoparticles and bi-functional molecular wires with dithiocarbamate anchoring groups. Finally, we demonstrate that flow cytometry can be used to significantly increase the proportion of AuNP dimers from an oligomer-rich polydisperse sample by size-selective sorting. 展开更多
关键词 flow cytometry characterization SORTING NANOPARTICLES gold nanoparticle dimers
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