Major strigolactones （SLs） produced by rice （Oryza sativa L. cv. Nipponbare） and tobacco （Nicotiana tabacum L. cv. Michinoku No. 1） were purified and their stereochemical structures were determined by comparing ...Major strigolactones （SLs） produced by rice （Oryza sativa L. cv. Nipponbare） and tobacco （Nicotiana tabacum L. cv. Michinoku No. 1） were purified and their stereochemical structures were determined by comparing with optically pure synthetic standards for their NMR and CD data and retention times and mass fragmentations in ESI-LC/MS and GC-MS. SLs purified from root exudates of rice plants were orobanchol, orobanchyl acetate, and ent-2＂-epi-5-deoxystr- igol. In addition to these SLs, 7-oxoorobanchyl acetate and the putative three methoxy-5~deoxystrigol isomers were detected by LC-MS/MS. The production of 7-oxoorobanchyl acetate seemed to occur in the early growth stage, as it was detected only in the root exudates collected during the first week of incubation. The root exudates of tobacco contained at least 11 SLs, including solanacol, solanacyl acetate, orobanchol, ent-2＂-epi-orobanchol, orobanchyl acetate, ent-2＇- epi-orobanchyl acetate, 5-deoxystrigol, ent-2＂-epi-5-deoxystrigol, and three isomers of putative didehydro-orobanchol whose structures remain to be clarified. Furthermore, two sorgolactone isomers but not sorgolactone were detected as minor SLs by LC-MS/MS analysis. It is intriguing to note that rice plants produced only orobanchol-type SLs, derived from ent-2＂-epi-5-deoxystrigol, but both orobanchol-type and strigol-type SLs, derived from 5-deoxystrigol were detected in tobacco plants.展开更多
Dear Editor,Strigolactones （SLs） are a class of carotenoid-derived plant hor-mones that regulate shoot branching among other developmental processes （Gomez-Roldan et al., 2008; Umehara et al., 2008; AI-Babili and B...Dear Editor,Strigolactones （SLs） are a class of carotenoid-derived plant hor-mones that regulate shoot branching among other developmental processes （Gomez-Roldan et al., 2008; Umehara et al., 2008; AI-Babili and Bouwmeester, 2015）. In addition, SLs are released by roots as a chemical signal attracting symbiotic arbuscular mycorrhizal fungi. However, this signal is also perceived by seeds of root parasitic weeds, announcing the presence of a host and triggering germination （AI-Babili and Bouwmeester, 2015）. Natural SLs consist of a butenolide ring （D ring） connected by an enol ether bridge to a tricyclic lactone （ABC rings） in canonical SLs, such as strigol, and to a variable, second moiety in non-canonical SLs, such as methyl carlactonoate.展开更多
(Molecular Plant11(10):1312-1314;October 2018;https://doi.org/10.1016/j.molp.2018.06.008)The amounts of CL and 3-H-CL shown in the original Figure 1D and Supplementary Figure 13 are inaccurate,due to an erroneous prep...(Molecular Plant11(10):1312-1314;October 2018;https://doi.org/10.1016/j.molp.2018.06.008)The amounts of CL and 3-H-CL shown in the original Figure 1D and Supplementary Figure 13 are inaccurate,due to an erroneous prep-aration and mixing up of samples.In addition,there is an error in the designation of the spectra of two peaks in the original Figure 1A,inwhich the spectrum designated as I should belong to peak II,andvice versa.We have repeated the related experiments and quanti-fication,and the corrected Figure 1 and Supplementary Figure 13 are shown below.展开更多
文摘Major strigolactones （SLs） produced by rice （Oryza sativa L. cv. Nipponbare） and tobacco （Nicotiana tabacum L. cv. Michinoku No. 1） were purified and their stereochemical structures were determined by comparing with optically pure synthetic standards for their NMR and CD data and retention times and mass fragmentations in ESI-LC/MS and GC-MS. SLs purified from root exudates of rice plants were orobanchol, orobanchyl acetate, and ent-2＂-epi-5-deoxystr- igol. In addition to these SLs, 7-oxoorobanchyl acetate and the putative three methoxy-5~deoxystrigol isomers were detected by LC-MS/MS. The production of 7-oxoorobanchyl acetate seemed to occur in the early growth stage, as it was detected only in the root exudates collected during the first week of incubation. The root exudates of tobacco contained at least 11 SLs, including solanacol, solanacyl acetate, orobanchol, ent-2＂-epi-orobanchol, orobanchyl acetate, ent-2＇- epi-orobanchyl acetate, 5-deoxystrigol, ent-2＂-epi-5-deoxystrigol, and three isomers of putative didehydro-orobanchol whose structures remain to be clarified. Furthermore, two sorgolactone isomers but not sorgolactone were detected as minor SLs by LC-MS/MS analysis. It is intriguing to note that rice plants produced only orobanchol-type SLs, derived from ent-2＂-epi-5-deoxystrigol, but both orobanchol-type and strigol-type SLs, derived from 5-deoxystrigol were detected in tobacco plants.
文摘Dear Editor,Strigolactones （SLs） are a class of carotenoid-derived plant hor-mones that regulate shoot branching among other developmental processes （Gomez-Roldan et al., 2008; Umehara et al., 2008; AI-Babili and Bouwmeester, 2015）. In addition, SLs are released by roots as a chemical signal attracting symbiotic arbuscular mycorrhizal fungi. However, this signal is also perceived by seeds of root parasitic weeds, announcing the presence of a host and triggering germination （AI-Babili and Bouwmeester, 2015）. Natural SLs consist of a butenolide ring （D ring） connected by an enol ether bridge to a tricyclic lactone （ABC rings） in canonical SLs, such as strigol, and to a variable, second moiety in non-canonical SLs, such as methyl carlactonoate.
文摘(Molecular Plant11(10):1312-1314;October 2018;https://doi.org/10.1016/j.molp.2018.06.008)The amounts of CL and 3-H-CL shown in the original Figure 1D and Supplementary Figure 13 are inaccurate,due to an erroneous prep-aration and mixing up of samples.In addition,there is an error in the designation of the spectra of two peaks in the original Figure 1A,inwhich the spectrum designated as I should belong to peak II,andvice versa.We have repeated the related experiments and quanti-fication,and the corrected Figure 1 and Supplementary Figure 13 are shown below.
