Under normal circumstances, spermatozoa are protected from the immune system by the blood-testis barrier. The breakdown of this barrier is the origin of the synthesis of antisperm antibodies (ASA). The presence of spe...Under normal circumstances, spermatozoa are protected from the immune system by the blood-testis barrier. The breakdown of this barrier is the origin of the synthesis of antisperm antibodies (ASA). The presence of sperm agglutinates in semen is characteristic of ASA. But is the presence of agglutinates in semen necessarily linked to the level of ASA in semen? The objective of this study was to assess the concentration of anti-sperm antibodies (ASA) in normozoosperms and infertile men with azoospermia. The biological material consisted of samples of human sperms: 30 samples with azoospermia and 32 with normozoospermia. The ASA assay was performed in seminal plasma using the DRG® Sperm Antibody ELISA (seminal plasma) kit (EIA-4249). The reading was carried out using a microplate reader at 450 nm. Data analysis was performed using Graph Pad Prism 7 software. The results obtained showed that the difference in ASA concentration between these two categories of sperm was not significant, with an average ASA level of 31.54 ± 2.45 U/mL in azoospermic ejaculate and 27.63 ± 1.51 U/mL in normozoosperms. Statistical analysis showed higher ASA concentrations in azoosperms with 6.67% of these declared positive. The ASA positivity rate made it possible to distinguish secretory azoospermias from obstructive ones. Also, the presence of ASA is not necessarily linked to the presence of agglutinates in the semen.展开更多
Objective:The relationship between mitochondrial DNA(mtDNA)polymorphisms and abnormalities in sperm quality has been the subject of several studies,with the objective of improving the treatment of male infertility.Thi...Objective:The relationship between mitochondrial DNA(mtDNA)polymorphisms and abnormalities in sperm quality has been the subject of several studies,with the objective of improving the treatment of male infertility.This study,which contributes to the identification of genetic markers of sperm abnormalities,was conducted to study mtDNA mutations in the asthenozoospermia profile.Methods:This case-control study included 30 patients with asthenozoospermia and 28 with normospermia after spermogram and spermocytogram analyses.After the extraction of total DNA from the spermatozoa of 58 ejaculates from these individuals using the phenol-chloroform method,the amplification of genes of interest in mtDNA using specific primers was performed by conventional polymerase chain reaction,and sequencing was used to detect mutations.Results:Male patients with asthenozoospermia in the tertiary sector had significantly more mutant-than wild-type(P=0.0005)MT-CO II genes.Similarly,for the same gene,males with asthenozoospermia and primary infertility had significantly more mutants than the wild-type(P=0.001).Sequencing revealed 29 mutations that were observed only with asthenozoospermia,which could be the basis for low sperm mobility.Conclusion:This study identified several mutations in mtDNA genes that could be considered genetic markers of asthenozoospermia if confirmed in a deeper study.展开更多
文摘Under normal circumstances, spermatozoa are protected from the immune system by the blood-testis barrier. The breakdown of this barrier is the origin of the synthesis of antisperm antibodies (ASA). The presence of sperm agglutinates in semen is characteristic of ASA. But is the presence of agglutinates in semen necessarily linked to the level of ASA in semen? The objective of this study was to assess the concentration of anti-sperm antibodies (ASA) in normozoosperms and infertile men with azoospermia. The biological material consisted of samples of human sperms: 30 samples with azoospermia and 32 with normozoospermia. The ASA assay was performed in seminal plasma using the DRG® Sperm Antibody ELISA (seminal plasma) kit (EIA-4249). The reading was carried out using a microplate reader at 450 nm. Data analysis was performed using Graph Pad Prism 7 software. The results obtained showed that the difference in ASA concentration between these two categories of sperm was not significant, with an average ASA level of 31.54 ± 2.45 U/mL in azoospermic ejaculate and 27.63 ± 1.51 U/mL in normozoosperms. Statistical analysis showed higher ASA concentrations in azoosperms with 6.67% of these declared positive. The ASA positivity rate made it possible to distinguish secretory azoospermias from obstructive ones. Also, the presence of ASA is not necessarily linked to the presence of agglutinates in the semen.
文摘Objective:The relationship between mitochondrial DNA(mtDNA)polymorphisms and abnormalities in sperm quality has been the subject of several studies,with the objective of improving the treatment of male infertility.This study,which contributes to the identification of genetic markers of sperm abnormalities,was conducted to study mtDNA mutations in the asthenozoospermia profile.Methods:This case-control study included 30 patients with asthenozoospermia and 28 with normospermia after spermogram and spermocytogram analyses.After the extraction of total DNA from the spermatozoa of 58 ejaculates from these individuals using the phenol-chloroform method,the amplification of genes of interest in mtDNA using specific primers was performed by conventional polymerase chain reaction,and sequencing was used to detect mutations.Results:Male patients with asthenozoospermia in the tertiary sector had significantly more mutant-than wild-type(P=0.0005)MT-CO II genes.Similarly,for the same gene,males with asthenozoospermia and primary infertility had significantly more mutants than the wild-type(P=0.001).Sequencing revealed 29 mutations that were observed only with asthenozoospermia,which could be the basis for low sperm mobility.Conclusion:This study identified several mutations in mtDNA genes that could be considered genetic markers of asthenozoospermia if confirmed in a deeper study.
