目的:评估非小细胞肺癌(non-small cell lung cancer,NSCLC)患者血清hsa-miR-107表达水平及临床价值,探究其潜在靶向调控机制。方法:基于全球NSCLC血清miRNA和组织mRNA基因芯片及测序数据集,评估hsa-miR-107综合表达水平,计算标准化平均...目的:评估非小细胞肺癌(non-small cell lung cancer,NSCLC)患者血清hsa-miR-107表达水平及临床价值,探究其潜在靶向调控机制。方法:基于全球NSCLC血清miRNA和组织mRNA基因芯片及测序数据集,评估hsa-miR-107综合表达水平,计算标准化平均差(standardized mean difference,SMD),鉴定差异表达miRNA、mRNA。以Kaplan-Meier生存分析评价hsa-miR-107的预后价值。参考萤光素酶报告基因测定和降解组测序结果对hsa-miR-107靶向mRNA进行鉴定,并对hsa-miR-107候选靶基因、NSCLC差异表达基因的交集进行功能注释、蛋白质交互分析。绘制汇总受试者操作特征曲线、Pearson相关系数散点图初步验证hsa-miR-107的靶向枢纽基因。结果:与155份正常人血清样本相比,hsa-miR-107在全球145份NSCLC患者血清样本中表达水平明显降低[SMD=-0.49(-0.73~-0.24)],且对NSCLC有中等区分能力[曲线下面积(area under the curve,AUC)=0.79,95%CI:0.75~0.82]。然而,血清hsa-miR-107高水平表达预示NSCLC患者预后较差(P<0.05,样本数:67)。hsa-miR-107靶基因主要富集于细胞周期通路、DNA复制通路、p53信号通路及细胞衰老通路,其中CCNE1、CDK1基因与hsa-miR-107呈显著负相关且均在NSCLC中呈高表达,被鉴定为hsa-miR-107靶向通路枢纽基因。结论:hsa-miR-107在NSCLC患者血清中低表达且其表达可预示NSCLC患者不良预后。低表达hsa-miR-107可能通过调控细胞周期通路基因CCNE1、CDK1促进NSCLC进展。展开更多
目的探讨mutT同系物1(MTH1)在非小细胞肺癌(NSCLC)患者中表达水平和临床意义.方法收集2013年3月至2017年3月在广西医科大学第一附属医院及柳州市人民医院手术治疗的诊断为NSCLC的197例患者的病理组织,采用免疫组织化学方法评估M T H1蛋...目的探讨mutT同系物1(MTH1)在非小细胞肺癌(NSCLC)患者中表达水平和临床意义.方法收集2013年3月至2017年3月在广西医科大学第一附属医院及柳州市人民医院手术治疗的诊断为NSCLC的197例患者的病理组织,采用免疫组织化学方法评估M T H1蛋白的表达,根据MTH1蛋白水平将患者分为MTH1高表达组、MTH1低表达组,分析MTH1表达与患者临床特征之间的关系.结果 MTH1高表达组与MTH1低表达组比较,男性、吸烟指数≥20包年、鳞癌、TNM分期>Ⅰ期、肿瘤直径≥3 cm、淋巴结转移、胸膜浸润阳性、淋巴管浸润和血管浸润比较,差异均有统计学意义(P值均<0.05).MTH1高表达者和M T H1低表达者5年总生存率分别为81.6%和92.3%(χ2=10.003,P=0.001);5年无瘤生存率分别为55.0%和83.7%(χ2=10.117,P<0.001).MTH1高表达(HR=1.713,95%CI:1.865~4.118,P=0.047)和淋巴结转移(H R=9.189,95%CI:2.824~39.457,P<0.001)是患者总生存率的独立危险因素;M T H1高表达(HR=1.605,95%CI:1.627~3.248,P=0.039)和淋巴结转移(H R=4.501,95%C I:1.942~11.546,P<0.001)是患者无瘤生存率的独立危险因素.结论在NSCLC中MTH1高表达患者表现出更高的恶性特征,其生存率低于MTH1低表达的患者.展开更多
Background Nowadays,there are published trials in regards to the comparison of caspofungin with liposomal amphotericin B （L-AmB）.However,these studies have a modest sample size and convey inconclusive results.The ai...Background Nowadays,there are published trials in regards to the comparison of caspofungin with liposomal amphotericin B （L-AmB）.However,these studies have a modest sample size and convey inconclusive results.The aim of this study was to review the efficacy and safety of caspofungin for the treatment of invasive fungal infections （IFIs）,compared with L-AmB.Methods Electronic databases （up to July 31,2013） PubMed and Embase databases,the Cochrane Library,and Google Scholar were searched to identify relevant trials of caspofungin and L-AmB.Analyses of efficacy and adverse outcomes were performed by relative risks （RRs） and 95% confidence intervals （C/s）.Heterogeneity was assessed by x2-test and the/2-statistic.Results Three trials were included in this meta-analysis with 1249 modified intention-to-treat （MITT） patients.The results showed that caspofungin produced equal efficacy in favorable overall response （RR=1.02,95% Cl 0.88-1.18; P=0.81） and mortality rate （RR=1.53,95% Cl 0.38-6.27,P=0.55）,safer in clinical adverse events （RR=0.20,95% Cl 0.08-0.54; P=0.001）,laboratory adverse events （RR=0.69,95% Cl 0.57-0.84; P=0.0002）,and discontinuation rate （RR=0.26,95% Cl 0.08-0.83,P=0.02）,compared with L-AmB in the treatment of patients with IFls.Conclusion Based on the results of this meta-analysis,it would appear that caspofungin was measured to have equal efficacy in clinical outcomes and safer in terms of adverse events.展开更多
Background Implanted medical catheter-related infections are increasing,hence a need for developing catheter polymers bonded to antimicrobials.We evaluated preventive effects of levofloxacin-impregnated catheters in c...Background Implanted medical catheter-related infections are increasing,hence a need for developing catheter polymers bonded to antimicrobials.We evaluated preventive effects of levofloxacin-impregnated catheters in catheterrelated Psuedomonas aeruginosa （strain PAO1） infection.Methods Drug release from levofloxacin-impregnated catheters was measured in vitro.Levofloxacin-impregnated catheters and polyvinyl chloride （PVC） catheters were immersed in 5 ml 50％ Luria Bertani medium containing 108 CFU/ml Pseudomonas aeruginosa then incubated for 6,12,24 or 48 hours at 37℃ when bacteria adhering to the catheters and bacteria in the growth culture medium were determined.Impregnated and PVC catheters were singly implanted subcutaneously in mice,50 μl （107CFU） of PAO1 was injected into catheters.After the first and fifth days challenge,bacterial counts on implanted catheters and in surrounding tissues were determined microbiologically.Bacterial colonization and biofilm formation on implanted catheters were assessed by scanning electron microscopy.Results Drug release from levofloxacin-impregnated catheters was rapid.Levofloxacin-impregnated catheters had significantly fewer bacteria compared to PVC in vitro.After first and fifth day of challenge,no or significantly fewer bacteria adhered to impregnated catheters or in surrounding tissues compared to PVC.Scanning electron microscopical images after first day displayed from none to significantly fewer bacteria adhering to impregnated implanted catheters,compared to bacteria and microcolonies adhering to PVC catheters.After the fifth day,no bacteria were found on impregnated catheters,compared to clusters surrounding mucus-like substance and coral-shaped biofilms with polymorphonuclear leukocyte on PVC catheters.After the first day of challenge,secretion occurred in all implanted catheters with surrounding tissues mildly hyperaemic and swollen.After the fifth day,minute secretions inside impregnated catheters and no inflammation in tissues,whereas purulent secretion inside PVC catheters and abscesses in surrounding tissues.Conclusion Levofloxacin-impregnated catheter is a promising new strategy for prevention of catheter-related Psuedomonas aeruginosa infection.展开更多
文摘目的:评估非小细胞肺癌(non-small cell lung cancer,NSCLC)患者血清hsa-miR-107表达水平及临床价值,探究其潜在靶向调控机制。方法:基于全球NSCLC血清miRNA和组织mRNA基因芯片及测序数据集,评估hsa-miR-107综合表达水平,计算标准化平均差(standardized mean difference,SMD),鉴定差异表达miRNA、mRNA。以Kaplan-Meier生存分析评价hsa-miR-107的预后价值。参考萤光素酶报告基因测定和降解组测序结果对hsa-miR-107靶向mRNA进行鉴定,并对hsa-miR-107候选靶基因、NSCLC差异表达基因的交集进行功能注释、蛋白质交互分析。绘制汇总受试者操作特征曲线、Pearson相关系数散点图初步验证hsa-miR-107的靶向枢纽基因。结果:与155份正常人血清样本相比,hsa-miR-107在全球145份NSCLC患者血清样本中表达水平明显降低[SMD=-0.49(-0.73~-0.24)],且对NSCLC有中等区分能力[曲线下面积(area under the curve,AUC)=0.79,95%CI:0.75~0.82]。然而,血清hsa-miR-107高水平表达预示NSCLC患者预后较差(P<0.05,样本数:67)。hsa-miR-107靶基因主要富集于细胞周期通路、DNA复制通路、p53信号通路及细胞衰老通路,其中CCNE1、CDK1基因与hsa-miR-107呈显著负相关且均在NSCLC中呈高表达,被鉴定为hsa-miR-107靶向通路枢纽基因。结论:hsa-miR-107在NSCLC患者血清中低表达且其表达可预示NSCLC患者不良预后。低表达hsa-miR-107可能通过调控细胞周期通路基因CCNE1、CDK1促进NSCLC进展。
文摘目的探讨mutT同系物1(MTH1)在非小细胞肺癌(NSCLC)患者中表达水平和临床意义.方法收集2013年3月至2017年3月在广西医科大学第一附属医院及柳州市人民医院手术治疗的诊断为NSCLC的197例患者的病理组织,采用免疫组织化学方法评估M T H1蛋白的表达,根据MTH1蛋白水平将患者分为MTH1高表达组、MTH1低表达组,分析MTH1表达与患者临床特征之间的关系.结果 MTH1高表达组与MTH1低表达组比较,男性、吸烟指数≥20包年、鳞癌、TNM分期>Ⅰ期、肿瘤直径≥3 cm、淋巴结转移、胸膜浸润阳性、淋巴管浸润和血管浸润比较,差异均有统计学意义(P值均<0.05).MTH1高表达者和M T H1低表达者5年总生存率分别为81.6%和92.3%(χ2=10.003,P=0.001);5年无瘤生存率分别为55.0%和83.7%(χ2=10.117,P<0.001).MTH1高表达(HR=1.713,95%CI:1.865~4.118,P=0.047)和淋巴结转移(H R=9.189,95%CI:2.824~39.457,P<0.001)是患者总生存率的独立危险因素;M T H1高表达(HR=1.605,95%CI:1.627~3.248,P=0.039)和淋巴结转移(H R=4.501,95%C I:1.942~11.546,P<0.001)是患者无瘤生存率的独立危险因素.结论在NSCLC中MTH1高表达患者表现出更高的恶性特征,其生存率低于MTH1低表达的患者.
文摘Background Nowadays,there are published trials in regards to the comparison of caspofungin with liposomal amphotericin B （L-AmB）.However,these studies have a modest sample size and convey inconclusive results.The aim of this study was to review the efficacy and safety of caspofungin for the treatment of invasive fungal infections （IFIs）,compared with L-AmB.Methods Electronic databases （up to July 31,2013） PubMed and Embase databases,the Cochrane Library,and Google Scholar were searched to identify relevant trials of caspofungin and L-AmB.Analyses of efficacy and adverse outcomes were performed by relative risks （RRs） and 95% confidence intervals （C/s）.Heterogeneity was assessed by x2-test and the/2-statistic.Results Three trials were included in this meta-analysis with 1249 modified intention-to-treat （MITT） patients.The results showed that caspofungin produced equal efficacy in favorable overall response （RR=1.02,95% Cl 0.88-1.18; P=0.81） and mortality rate （RR=1.53,95% Cl 0.38-6.27,P=0.55）,safer in clinical adverse events （RR=0.20,95% Cl 0.08-0.54; P=0.001）,laboratory adverse events （RR=0.69,95% Cl 0.57-0.84; P=0.0002）,and discontinuation rate （RR=0.26,95% Cl 0.08-0.83,P=0.02）,compared with L-AmB in the treatment of patients with IFls.Conclusion Based on the results of this meta-analysis,it would appear that caspofungin was measured to have equal efficacy in clinical outcomes and safer in terms of adverse events.
基金This study was supported in part by grants from the National Natural Science Foundation of China （No. 30760084） and by Guangxi Health Department Self Financing Scientific Research, China （No. Z2008154）.
文摘Background Implanted medical catheter-related infections are increasing,hence a need for developing catheter polymers bonded to antimicrobials.We evaluated preventive effects of levofloxacin-impregnated catheters in catheterrelated Psuedomonas aeruginosa （strain PAO1） infection.Methods Drug release from levofloxacin-impregnated catheters was measured in vitro.Levofloxacin-impregnated catheters and polyvinyl chloride （PVC） catheters were immersed in 5 ml 50％ Luria Bertani medium containing 108 CFU/ml Pseudomonas aeruginosa then incubated for 6,12,24 or 48 hours at 37℃ when bacteria adhering to the catheters and bacteria in the growth culture medium were determined.Impregnated and PVC catheters were singly implanted subcutaneously in mice,50 μl （107CFU） of PAO1 was injected into catheters.After the first and fifth days challenge,bacterial counts on implanted catheters and in surrounding tissues were determined microbiologically.Bacterial colonization and biofilm formation on implanted catheters were assessed by scanning electron microscopy.Results Drug release from levofloxacin-impregnated catheters was rapid.Levofloxacin-impregnated catheters had significantly fewer bacteria compared to PVC in vitro.After first and fifth day of challenge,no or significantly fewer bacteria adhered to impregnated catheters or in surrounding tissues compared to PVC.Scanning electron microscopical images after first day displayed from none to significantly fewer bacteria adhering to impregnated implanted catheters,compared to bacteria and microcolonies adhering to PVC catheters.After the fifth day,no bacteria were found on impregnated catheters,compared to clusters surrounding mucus-like substance and coral-shaped biofilms with polymorphonuclear leukocyte on PVC catheters.After the first day of challenge,secretion occurred in all implanted catheters with surrounding tissues mildly hyperaemic and swollen.After the fifth day,minute secretions inside impregnated catheters and no inflammation in tissues,whereas purulent secretion inside PVC catheters and abscesses in surrounding tissues.Conclusion Levofloxacin-impregnated catheter is a promising new strategy for prevention of catheter-related Psuedomonas aeruginosa infection.
