To investigate the morphologic changes in Supramid Extra suture harvested from patients displaying recurrent ptosis who had undergone a frontalis suspension f or congenital ptosis. Observational case series. Using sca...To investigate the morphologic changes in Supramid Extra suture harvested from patients displaying recurrent ptosis who had undergone a frontalis suspension f or congenital ptosis. Observational case series. Using scanning electron microsc opy, we examined the morphologic changes in Supramid Extra (S. Jackson Inc, Alex andria, Virginia, USA) harvested from 20 consecutive patients (23 eyelids) with recurrent ptosis who had undergone frontalis suspension. Disintegration was grad ed as mild, moderate, or severe. Examination of unused Supramid showed the smoot h surface of the sheath and round, well de marcated polyfilaments on cross sec tion. However, implanted Supramid showed different degrees of disintegration. Th e surface of the sheath had a rough texture with cracks and peeling. On cross se ction, an accumulation of debris among the polyfilaments and irregular architect ures with decreased diameters of the polyfilaments were noted in some cases. On the surface of polyfilaments, different degrees of debris were noted in all case s. The degree of the changes was not exactly correlated with either the time of the recurrence or the duration of implantation. However, as the duration of impl anted Supramid increased, greater changes on the surface of the sheath were note d. With implantation of less than 37 months, mild changes (44%) were predominan t; between 37 and 48 months, moderate changes (60%) were noted, and with more t han 48 months of implantation, severe changes (44%) were predominant. Supramid undergoes morphologic degradation by hydrolysis after implantation. This change might be considered one of the possible factors that facilitate the recurrence o f ptosis.展开更多
Purpose:The purpose of the study was to evaluate the outcome of the use of the temporary amniotic membrane patch(TAMP)for the treatment of primary pterygium and to investigate the mechanisms of reducing the recurrence...Purpose:The purpose of the study was to evaluate the outcome of the use of the temporary amniotic membrane patch(TAMP)for the treatment of primary pterygium and to investigate the mechanisms of reducing the recurrence rate.Methods:Twenty eyes in 20 patients with primary pterygium underwent pterygium excision followed by TAMP for 5 days.Removed amniotic membrane(AM)was immunostained with primary antibodies CD34,c-Kit,STRO-1 and AC133.Results:Within the period of follow-up(53.3± 13.8 months),all the eyes showed a smooth ocular surface without recurrence of pterygium.Different grades of CD34,c-Kit,STRO-1 and AC133 positive stem and progenitor cells infiltrated or attached to the stroma of patched AM,with more spindle-shaped c-Kit cells than ovoid-shaped CD34 and AC133 cells.Conclusion:The temporary amniotic membrane patch is an effective and safe procedure for the treatment of primary pterygium.Absorbing excessive stem and progenitor cells may be one of the mechanisms of reducing the recurrence rate using AM.展开更多
文摘To investigate the morphologic changes in Supramid Extra suture harvested from patients displaying recurrent ptosis who had undergone a frontalis suspension f or congenital ptosis. Observational case series. Using scanning electron microsc opy, we examined the morphologic changes in Supramid Extra (S. Jackson Inc, Alex andria, Virginia, USA) harvested from 20 consecutive patients (23 eyelids) with recurrent ptosis who had undergone frontalis suspension. Disintegration was grad ed as mild, moderate, or severe. Examination of unused Supramid showed the smoot h surface of the sheath and round, well de marcated polyfilaments on cross sec tion. However, implanted Supramid showed different degrees of disintegration. Th e surface of the sheath had a rough texture with cracks and peeling. On cross se ction, an accumulation of debris among the polyfilaments and irregular architect ures with decreased diameters of the polyfilaments were noted in some cases. On the surface of polyfilaments, different degrees of debris were noted in all case s. The degree of the changes was not exactly correlated with either the time of the recurrence or the duration of implantation. However, as the duration of impl anted Supramid increased, greater changes on the surface of the sheath were note d. With implantation of less than 37 months, mild changes (44%) were predominan t; between 37 and 48 months, moderate changes (60%) were noted, and with more t han 48 months of implantation, severe changes (44%) were predominant. Supramid undergoes morphologic degradation by hydrolysis after implantation. This change might be considered one of the possible factors that facilitate the recurrence o f ptosis.
文摘Purpose:The purpose of the study was to evaluate the outcome of the use of the temporary amniotic membrane patch(TAMP)for the treatment of primary pterygium and to investigate the mechanisms of reducing the recurrence rate.Methods:Twenty eyes in 20 patients with primary pterygium underwent pterygium excision followed by TAMP for 5 days.Removed amniotic membrane(AM)was immunostained with primary antibodies CD34,c-Kit,STRO-1 and AC133.Results:Within the period of follow-up(53.3± 13.8 months),all the eyes showed a smooth ocular surface without recurrence of pterygium.Different grades of CD34,c-Kit,STRO-1 and AC133 positive stem and progenitor cells infiltrated or attached to the stroma of patched AM,with more spindle-shaped c-Kit cells than ovoid-shaped CD34 and AC133 cells.Conclusion:The temporary amniotic membrane patch is an effective and safe procedure for the treatment of primary pterygium.Absorbing excessive stem and progenitor cells may be one of the mechanisms of reducing the recurrence rate using AM.
