Objective:To investigate on patients leishmanial infections in Jask County.Methods: Impression smears were prepared from patients in 2008,all,were chequed for leishmanial infection by microscopy and molecular assays.W...Objective:To investigate on patients leishmanial infections in Jask County.Methods: Impression smears were prepared from patients in 2008,all,were chequed for leishmanial infection by microscopy and molecular assays.Whole D.NA was extracted using Proteinase K and Phenol/Chloroform/Isoamyl alcohol method.The variable segment on minicircles of kineloplasl DIVA was amplified via a Nested-PCR technique using species-specific primers(LIN R4-LIN 17 -Lin 19).Results:A total of 40 smears were prepared from 20 patients,from which,eight samples (40%) were positive for leishman body by microscopic method,while,18 samples(90%) were positive,molecularly.The parasite was identified as Leishmania major(L major).Conclusions: Zoonotic or Rural cutaneous leishmaniasis is endemic in Jask County whose pathogen is L major.Molecular assays using specific primers are very accurate and more sensitive and specific than microscopy which is time consuming and needs master mieroscopists.展开更多
基金the result of research plan(No.M/P/251 dated 1-1-2008) carried out under the auspices of HUMS
文摘Objective:To investigate on patients leishmanial infections in Jask County.Methods: Impression smears were prepared from patients in 2008,all,were chequed for leishmanial infection by microscopy and molecular assays.Whole D.NA was extracted using Proteinase K and Phenol/Chloroform/Isoamyl alcohol method.The variable segment on minicircles of kineloplasl DIVA was amplified via a Nested-PCR technique using species-specific primers(LIN R4-LIN 17 -Lin 19).Results:A total of 40 smears were prepared from 20 patients,from which,eight samples (40%) were positive for leishman body by microscopic method,while,18 samples(90%) were positive,molecularly.The parasite was identified as Leishmania major(L major).Conclusions: Zoonotic or Rural cutaneous leishmaniasis is endemic in Jask County whose pathogen is L major.Molecular assays using specific primers are very accurate and more sensitive and specific than microscopy which is time consuming and needs master mieroscopists.
