Effect of Pre-Harvest Application of Gibberellic Acid on Fruit Quality and Shelf Life of Mandarin (Citrus reticulata Blanco)

Mandarin (Citrus reticulata Blanco) is a promising fruit crop gaining popularity for its human nutrition and economic importance in Nepal. The qualitative losses during pre-harvest stage hinder the productivity and subsequently shorten the on-tree storability. An experiment was conducted to assess the effect of gibberellic acid on quality and shelf life of the mandarin fruit. GA3 at 10, 20, and 30 ppm as against of control were evaluated. Observations on fruit weight (g), fruit firmness (kg/cm2), rind colour (1 - 5 index), juice recovery (%), TSS/acid ratio, PLW (%), decay loss (%), and ascorbic acid (mg/100 ml) were recorded at three harvesting dates i.e. 20 Nov, 5 Dec, and 20 Dec and storage condition. It has been revealed that the fruits treated with GA3 at 20 ppm retained higher fruit weight (128.6 g), more firmness (3.54 kg/cm2), better juice recovery (57.75%), and greater TSS/acid ratio (21.24) at the end of study (20 December). The PLW was found less with GA3 at 30 ppm in both ambient (5.17%) and cellar (6.69%) condition as against untreated fruits (9.52% and 11.76%). Similarly, the decay loss was minimum in the fruits treated with GA3 at 30 ppm both with ambient (1.02%) and cellar condition (8.21%) as against control with ambient (5.54%) and cellar (21.58%).

Variation of Physiochemical Components of Acid Lime (<i>Citrus aurantifolia</i>Swingle) Fruits at Different Sides of the Tree in Nepal

Acid Lime (Citrus aurantifolia Swingle) is one of the important commercial fruits cultivated from terai to high hill of Nepal. It is an important source of vitamin “C” (ascorbic acid) for human nutrition. Amount of juice, TSS (Total soluble solids), TA (Titrable acid) and vitamin C are the determining factors of quality of acid lime fruits which may vary according to bearing sides of the trees. The main objective of this study is, to determine the variation of fruit quality at different sides of the tree. Total of 15 bearing trees were selected randomly (5 trees per site) from three different agro ecological domain representing terai (1200 m asl) and samples were collected from the selected trees. Randomly ten fruits (from east, west, centre, north and south sides) were collected from each tree and analyzed for amount of vitamin C, TSS, TA and juice. Highest ascorbic acid 79.6 mg and 69.9 mg was observed in south side fruits whereas lowest 62.8 mg and 55.1 mg was observed in centre fruits in the high and mid hills zone respectively, but in terai, highest ascorbic acid 58.7 mg was observed in north side and lowest 41.8 mg was observed in centre. Highest amount of juice 43.9% was observed in south side fruits and lowest 36.6% in centre fruits, but amount of TSS 8.2% and TA 7.2% was observed in south side fruits and lowest 7.3% TSS and 7.0% TA was observed in centre fruits in high hills. In terai highest TSS 8.3% and TA 7.4% was observed in north side fruits and lowest TSS 7.3% and TA 6.7% was observed in centre fruits. Variation of TSS, TA percent and ascorbic acids was observed according to the agro ecological zone.

Effect of Altitude and Maturity Stages on Quality Attributes of Mandarin (Citrus reticulata Blanco)

Mandarin (Citrus reticulata Blanco) is a premier fruit crop which ranks in first position of the total fruit industry in Nepal. Studies were conducted to assess the maturity indices and quality parameters of mandarin at three altitudes i.e. 1300 m·asl, 1000 m·asl, and 700 m·asl with six maturity stages i.e. 11 Oct, 21 Oct, 31 Oct, 10 Nov, 20 Nov, and 30 Nov. The most important and reliable judging criteria of fruit maturity in mandarin i.e. fruit weight, external fruit colour, firmness, TSS, acidity, and TSS/Acid ratio, and vitamin C were experimented. The experiment results revealed that 1000 m·asl location was showed the highest fruit weight (104.9 g) and juice percentage (55.23) followed by 1300 m·asl altitude (99.5 g and 53.75% respectively) at 20th of November. The maturity advanced at lower altitude with 50 percent yellow orange rind colour, 10.98 TSS/acid ratio and 4 kg/cm2 firmness from 10th November at lower altitudes whereas it was appeared in 20th November at higher altitudes. The TSS/acid ratio was significantly higher (10.98) in the fruits of 700 m·asl as compared to 1300 m·asl (9.76) on 10th November, however, on 20th November 1300 m·asl showed the highest ratio (17.76). The fruit weight, juice content, TSS was found in increasing trends up to 20 November and then showed constant and decreasing trend. However, TA, firmness and vitamin C were showed in decreasing trends with time. The 1000 - 1300 m·asl was the best location for the mandarin production and 10-20 November was the best period for the mandarin harvesting for optimum maturity.

Study of Fruit Diversity and Selection of Elite Acid Lime (<i>Citrus aurantifolia Swingle</i>) Genotypes in Nepal

Acid lime (Citrus aurantifolia) fruits are cultivated in terai, mid hill and high hill districts of Nepal. It is cross-pollinated crop and has a high variation in fruit characters within the species. Fruit character is the main basis of genotype selection. Study of fruit diversity is of utmost importance to select the elite genotypes for breeding and variety development program. Therefore, total of 620 fruit samples were collected and evaluated in the main production season of the year 2010 and 2011, from 62 bearing trees. Samples were collected from three agro-ecological domains, representing terai, mid hill and high hill areas of eastern Nepal. Elite landraces were selected on the basis of scoring fruit characters. High variation in vitamin C was observed between the terai (57.4 mg) and high hill (72.5 mg) samples. Amount of juice was 36.8% in mid hill and 44.1% in terai samples. Higher percent of juice and total soluble solids (TSS) observed in terai samples but tritable acidity (TA) and vitamin C was low as compare to high hills. There was significantly negative correlation found in fruit weight, fruit diameter, juice percent and seed number, whereas significantly positive correlation was in pulp and vitamin C, with altitude range. Total landraces were separated in five major cluster group. Variation in the level of similarity was observed among the cluster groups. The variability distance of fruit characters was highest in cluster group II (–17.45%) and lowest in cluster group V (58.5%). On the basis of scoring number of fruit characters, total four elite genotypes i.e. LD-49 from mid hills, LD-23 and LT-17 from higher hills and LM-44 from terai were selected for conservation, breeding and variety development purpose.

Genetic Diversity Assessment of Acid Lime (<i>Citrus aurantifolia</i>Swingle) Landraces in Nepal, Using SSR Markers

Acid lime (Citrus aurantifolia) is an important commercial fruits crop, cultivated in terai to high hills of Nepal. High variation of acid lime fruits are observed in existing landraces due to crossing within the other citrus species. Determination of genetic variation is important to the plant breeders for development of high yielding variety and hybrids. Therefore, an attempt has been made to study the genetic diversity of 62 acid lime landraces, collected from different altitudinal range in the eastern part of Nepal, using SSR markers. Twelve Simple Sequence Repeat (SSR) primer pairs were used to assess the genetic diversity of acid lime. The average genetic similarity level among the 62 accessions was 0.77, ranging from 0.54 to 1.0 and separated five major cluster groups. Total of 33 alleles were detected by eleven primer pairs and size of alleles ranged from 50 to 225. Average polymorphic information content (PIC) value was 0.50, whereas highest 0.75 and lowest 0.18 was observed in CAT01 and GT03 loci respectively. The results of the study clearly indicated that, SSR markers are highly polymorphic and more informative for the assessment of genetic diversity of acid lime landraces.

Effect of Postharvest Treatments on Quality and Shelf Life of Mandarin (Citrus reticulata Blanco)

Nepal produces large quantity of mandarin (Citrus reticulata Blanco) annually but faces big losses due to improper postharvest treatments. The experiment was carried out during 2012-2013 with the objective of assessing the effect of different postharvest treatments on quality and shelf life of mandarin. The postharvest treatments were: wax emulsion at 10%, bavistin at 0.1%, wax at 10% with bavistin at 0.1%, calcium chloride at 1%, Jeevatu at 5% along with untreated fruits as a control. The experiment was conducted for four weeks of storage at ambient condition in Nepal Agricultural Research Council, Khumaltar, Kathmandu. Wax in combination with bavistin was found as the most effective in reducing the physiological loss in weight, whereas bavistin proved to reduce decay loss up to four weeks of storage. The fruits treated with wax alone and with combination of bavistin retained maximum firmness, juice percentage, vitamin C and palatability rating during the storage. The minimum total soluble solid and maximum titrable acidity were recorded in the fruits treated with wax plus bavistin. The present findings indicate that mandarin can be stored up to four weeks when treated with wax as well as wax with bavistin in the condition with 14&deg;C - 18&deg;C temperature and 45% - 73% relative humidity.