Lycium barbarum polysaccharides(LBPs)are the major polysaccharides extracted from L.barbarum,which is used in traditional Chinese medicine(TCM)for treating diseases.Studies have shown that LBPs have important biologic...Lycium barbarum polysaccharides(LBPs)are the major polysaccharides extracted from L.barbarum,which is used in traditional Chinese medicine(TCM)for treating diseases.Studies have shown that LBPs have important biological activities,such as antioxidation,anti-aging,neuroprotection,immune regulation.LBPs inhibit oxidative stress,improve neurodegeneration and stroke-induced neural injury,increase proliferation and differentiation of neural stem cell,and promote neural regeneration.Here we have reviewed latest advances in the biomedical activities of LBPs and improved methods for the isolation,extraction,and purification of LBPs.Then,new discoveries to decrease oxidative stress and cellular apoptosis,inhibit aging progress,and improve neural repair in neurodegeneration and ischemic brain injury have been discussed in detail through in vitro cell culture and in vivo animal studies.Importantly,the molecular mechanisms of LBPs in playing neuroprotective roles are further explored.Lastly,we discuss the perspective of LBPs as biomedical compounds in TCM and modern medicine and provide the experimental and theoretical evidence to use LBPs for the treatment of aging-related neurological diseases and stroke-induced neural injuries.展开更多
The investigation of sex determination in fish is not only of great scientific value,but also has important practical significance,especially in fishes grown in aquaculture exhibiting obvious growth-related sexual dim...The investigation of sex determination in fish is not only of great scientific value,but also has important practical significance,especially in fishes grown in aquaculture exhibiting obvious growth-related sexual dimorphism,such as the large yellow croaker(Larimichthys crocea).While growth of the female large yellow croaker is faster than that of the male,little information exists on the mechanisms that drive the differentiation of the testis and ovary in this species.In this study,the gene expression profiles of large yellow croaker testis and ovary were analysed using RNA-Seq technology.A total of 222 million reads were mapped to the reference genome(89.80%).The differential gene expression analysis identified 13 genes specifically expressed in ovary and 897 specific to testis.Among these,were candidate sex determining genes,such as Dmrt1,Gsdf,Sox9,Amh.This study explored gene expression profiles in large yellow croaker gonads at the transcriptome level by RNA-Seq technology.We found many DEGs related to the development of each specific organ,including the candidate testis differentiation genes Dmrt1,Gsdf,Sox9,Sox3,Amh and ovary differentiation gene Foxl2 by analogy with other fish species,providing essential foundation clarifying the molecular mechanism involved in early sex differentiation.展开更多
文摘Lycium barbarum polysaccharides(LBPs)are the major polysaccharides extracted from L.barbarum,which is used in traditional Chinese medicine(TCM)for treating diseases.Studies have shown that LBPs have important biological activities,such as antioxidation,anti-aging,neuroprotection,immune regulation.LBPs inhibit oxidative stress,improve neurodegeneration and stroke-induced neural injury,increase proliferation and differentiation of neural stem cell,and promote neural regeneration.Here we have reviewed latest advances in the biomedical activities of LBPs and improved methods for the isolation,extraction,and purification of LBPs.Then,new discoveries to decrease oxidative stress and cellular apoptosis,inhibit aging progress,and improve neural repair in neurodegeneration and ischemic brain injury have been discussed in detail through in vitro cell culture and in vivo animal studies.Importantly,the molecular mechanisms of LBPs in playing neuroprotective roles are further explored.Lastly,we discuss the perspective of LBPs as biomedical compounds in TCM and modern medicine and provide the experimental and theoretical evidence to use LBPs for the treatment of aging-related neurological diseases and stroke-induced neural injuries.
基金M.C and Z.W conceived and designed the study,J.X and K.C conducted samples collection and molecular biology experiments,S.X and Y.Z performed the bioinformatics analysis,J.X and S.X wrote the manuscript.This work was funded by the National Natural Science Foundation of China(31272653,41706157)the Natural Science Foundation of Fujian Province(2017J01449).
文摘The investigation of sex determination in fish is not only of great scientific value,but also has important practical significance,especially in fishes grown in aquaculture exhibiting obvious growth-related sexual dimorphism,such as the large yellow croaker(Larimichthys crocea).While growth of the female large yellow croaker is faster than that of the male,little information exists on the mechanisms that drive the differentiation of the testis and ovary in this species.In this study,the gene expression profiles of large yellow croaker testis and ovary were analysed using RNA-Seq technology.A total of 222 million reads were mapped to the reference genome(89.80%).The differential gene expression analysis identified 13 genes specifically expressed in ovary and 897 specific to testis.Among these,were candidate sex determining genes,such as Dmrt1,Gsdf,Sox9,Amh.This study explored gene expression profiles in large yellow croaker gonads at the transcriptome level by RNA-Seq technology.We found many DEGs related to the development of each specific organ,including the candidate testis differentiation genes Dmrt1,Gsdf,Sox9,Sox3,Amh and ovary differentiation gene Foxl2 by analogy with other fish species,providing essential foundation clarifying the molecular mechanism involved in early sex differentiation.
