A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length...A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length, snout length, mouth length, mouth width and eye diameter） was analyzed using SPSS software. And the results indicated that all the characters had a characteristic of continuous variation which belonged to the typical quantitative characters or polygenie inheri- tance characters, and fitted the normal distribution. Meanwhile, GLM procedure was used to analyze the correlation between the 22 microsatellites and 7 characters ; and multiple comparisons of the markers significantly correlative to these characters were carried out based on the genotypes. Results showed in that among the 22 micrasatellite loci, there were 10 markers significantly correlative to at least one character. The maximum number of markers related to mouth length and eye diame- ter was 7. Results of multiple comparisons showed that there were signifieant differences of genotypes from the same character in every marker. Screening of these markers provided basis for the further study on molecular marker-assisted breeding.展开更多
The ability to detect and identify quantitative trait loci (QTLs) in a single population is often limited. Analyzing multiple populations in QTL analysis improves the power of detecting QTLs and provides a better unde...The ability to detect and identify quantitative trait loci (QTLs) in a single population is often limited. Analyzing multiple populations in QTL analysis improves the power of detecting QTLs and provides a better understanding of their functional allelic variation and distribution. In this study, a consensus map of the common carp was constructed, based on four populations, to compare the distribution and variation of QTLs. The consensus map spans 2371.6 cM across the 42 linkage groups and comprises 257 microsatellites and 421 SNPs, with a mean marker interval of 3.7 cM/marker. Sixty-seven QTLs affecting four growth traits from the four populations were mapped to the consensus map. Only one QTL was common to three populations, and nine QTLs were detected in two populations. However, no QTL was common to all four populations. The results of the QTL comparison suggest that the QTLs are responsible for the phenotypic variability observed for these traits in a broad array of common carp germplasms. The study also reveals the different genetic performances between major and minor genes in different populations.展开更多
基金Supported by the National Science and Technology Support Plan Project(2012BDA25B10)the Science and Technology Project of Heilongjiang Province(GZ11B201)+1 种基金the Fundamental Research Fund for Central Public Welfare Research Institutes(201109)the Open Project of Key Laboratory of Freshwater Aquatic Biotechnology and Breeding,Ministry of Agriculture(2012NYBSJ-03)
文摘A total of 22 mierosatellite DNA markers were selected to analyze the genomic DNA of 176 wild population progenies from 4 families. The normal distri- bution of seven characters （body length, fork length, head length, snout length, mouth length, mouth width and eye diameter） was analyzed using SPSS software. And the results indicated that all the characters had a characteristic of continuous variation which belonged to the typical quantitative characters or polygenie inheri- tance characters, and fitted the normal distribution. Meanwhile, GLM procedure was used to analyze the correlation between the 22 microsatellites and 7 characters ; and multiple comparisons of the markers significantly correlative to these characters were carried out based on the genotypes. Results showed in that among the 22 micrasatellite loci, there were 10 markers significantly correlative to at least one character. The maximum number of markers related to mouth length and eye diame- ter was 7. Results of multiple comparisons showed that there were signifieant differences of genotypes from the same character in every marker. Screening of these markers provided basis for the further study on molecular marker-assisted breeding.
基金supported by the National Basic Research Program of China (2010CB126305)the Special Fund for Agro-scientific Research in the Public Interest (200903045)National High Technology Research and Development Program of China (2011AA100402)
文摘The ability to detect and identify quantitative trait loci (QTLs) in a single population is often limited. Analyzing multiple populations in QTL analysis improves the power of detecting QTLs and provides a better understanding of their functional allelic variation and distribution. In this study, a consensus map of the common carp was constructed, based on four populations, to compare the distribution and variation of QTLs. The consensus map spans 2371.6 cM across the 42 linkage groups and comprises 257 microsatellites and 421 SNPs, with a mean marker interval of 3.7 cM/marker. Sixty-seven QTLs affecting four growth traits from the four populations were mapped to the consensus map. Only one QTL was common to three populations, and nine QTLs were detected in two populations. However, no QTL was common to all four populations. The results of the QTL comparison suggest that the QTLs are responsible for the phenotypic variability observed for these traits in a broad array of common carp germplasms. The study also reveals the different genetic performances between major and minor genes in different populations.
