Grouper and snapper are the potential fishery commodity in Indonesia with a high economic value, as well as an export commodity. A common disease in grouper and snapper aquaculture is vibriosis. Vibriosis is a disease...Grouper and snapper are the potential fishery commodity in Indonesia with a high economic value, as well as an export commodity. A common disease in grouper and snapper aquaculture is vibriosis. Vibriosis is a disease caused by bacteria of the genus Vibrio. The aim of study was to compare between phenotypic and genotypic identification of Vibrio isolated from Batam and Mataram, Indonesia. Bacteria were isolated from anterior kidney and eye of fish, then grown in thiosulfate-citrate-bile salts-sucrose （TCBS） and incubated in room temperature （25-28 ~C） for 24 h, and identified using morphology and biochemical test. Bacterial isolates were extracted, amplified and sequenced on 16S rRNA region. Phylogenetic tree of bacteria was constructed using neighbor-joining and maximum-parsimony methods. The phenotypic identification was found six isolates of Vibrio from Batam, such as K alginolyticus, V. carchariae, K damselae, V. fluvialis, V. furnissii and K parahaemolyticus. Three isolates were found from Mataram, such as 1I. alginolyticus, V. carchariae and V. fluvialis. Blast analysis showed isolates of V. alginolyticus_btm and V. carchariae_btm homolog to V. parahaemolyticus strain DAHMV3; isolates of V. damselae_btm and K alginolyticus_mtr homolog to V. neocaledonicus strain MS1; isolates of V. parahaemolyticus__btm and V. furnisii_btm homolog with Photobacterium damselae subsp, damselae strain： 04Ya311 and isolate of K fluvialis_mtr homolog to V. azureus strain MMRF532, respectively. All phenotypic identification was not supported by molecular identification on 16S rRNA region. It was suggested that phenotypic identification should be supported by molecular examination, especially in identification of Vibrio species.展开更多
Streptococcus iniae has been detected in several regions in Indonesia, such as Bali, Jambi and Papua. Outbreak of meningoencephalitis and septicemia was still often found in Papua. The aim of study was to compare phen...Streptococcus iniae has been detected in several regions in Indonesia, such as Bali, Jambi and Papua. Outbreak of meningoencephalitis and septicemia was still often found in Papua. The aim of study was to compare phenotype, genotype and histopathology of S. iniae from Papua. Bacteria was identified using morphological and biochemical tests. It was also extracted, amplified and sequenced to see genotypic characters. The primer used were 5＇-AGAGTTTGATCCTGGCT-3＇ （24F） and 5＇-AAGGGAGGTGATCCAGCCGCA-3＇ （1540R） in 16S rRNA region. Result of sequencing was analyzed by the neighbor joining and maximum parsimony methods. The identification result of isolate from Papua was S. iniae. The amplification result was a sharp band--l,500 bp band in 16S rRNA region. The phylogenetic tree showed that isolate from Papua was closely related to S. iniae strains CMS005 from Guangxi China. Bacterial clumps of S. iniae was firstly found in the blood vessel of liver at the 3rd day after infection and then caused the inflammation to spleen, heart, brain, kidney and gut at the 7th day post infection.展开更多
文摘Grouper and snapper are the potential fishery commodity in Indonesia with a high economic value, as well as an export commodity. A common disease in grouper and snapper aquaculture is vibriosis. Vibriosis is a disease caused by bacteria of the genus Vibrio. The aim of study was to compare between phenotypic and genotypic identification of Vibrio isolated from Batam and Mataram, Indonesia. Bacteria were isolated from anterior kidney and eye of fish, then grown in thiosulfate-citrate-bile salts-sucrose （TCBS） and incubated in room temperature （25-28 ~C） for 24 h, and identified using morphology and biochemical test. Bacterial isolates were extracted, amplified and sequenced on 16S rRNA region. Phylogenetic tree of bacteria was constructed using neighbor-joining and maximum-parsimony methods. The phenotypic identification was found six isolates of Vibrio from Batam, such as K alginolyticus, V. carchariae, K damselae, V. fluvialis, V. furnissii and K parahaemolyticus. Three isolates were found from Mataram, such as 1I. alginolyticus, V. carchariae and V. fluvialis. Blast analysis showed isolates of V. alginolyticus_btm and V. carchariae_btm homolog to V. parahaemolyticus strain DAHMV3; isolates of V. damselae_btm and K alginolyticus_mtr homolog to V. neocaledonicus strain MS1; isolates of V. parahaemolyticus__btm and V. furnisii_btm homolog with Photobacterium damselae subsp, damselae strain： 04Ya311 and isolate of K fluvialis_mtr homolog to V. azureus strain MMRF532, respectively. All phenotypic identification was not supported by molecular identification on 16S rRNA region. It was suggested that phenotypic identification should be supported by molecular examination, especially in identification of Vibrio species.
文摘Streptococcus iniae has been detected in several regions in Indonesia, such as Bali, Jambi and Papua. Outbreak of meningoencephalitis and septicemia was still often found in Papua. The aim of study was to compare phenotype, genotype and histopathology of S. iniae from Papua. Bacteria was identified using morphological and biochemical tests. It was also extracted, amplified and sequenced to see genotypic characters. The primer used were 5＇-AGAGTTTGATCCTGGCT-3＇ （24F） and 5＇-AAGGGAGGTGATCCAGCCGCA-3＇ （1540R） in 16S rRNA region. Result of sequencing was analyzed by the neighbor joining and maximum parsimony methods. The identification result of isolate from Papua was S. iniae. The amplification result was a sharp band--l,500 bp band in 16S rRNA region. The phylogenetic tree showed that isolate from Papua was closely related to S. iniae strains CMS005 from Guangxi China. Bacterial clumps of S. iniae was firstly found in the blood vessel of liver at the 3rd day after infection and then caused the inflammation to spleen, heart, brain, kidney and gut at the 7th day post infection.
