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后膜骨架蛋白1a在小鼠脑缺血再灌注损伤中的作用 被引量:1
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作者 杨小利 殷亚楠 +5 位作者 胡文淼 张理斐 童丽 孙志平 程坤 王亚君 《中国现代医学杂志》 CAS 2018年第4期11-16,共6页
目的探讨后膜骨架蛋白1a(Homer 1a)在小鼠脑缺血再灌注损伤中的作用。方法以Homer1a基因敲除小鼠为研究对象,复制脑缺血再灌注小鼠模型,根据行为学评分标准评价小鼠脑缺血再灌注的损伤程度。运用TTC染色法观察计算小鼠脑梗死体积。Tune... 目的探讨后膜骨架蛋白1a(Homer 1a)在小鼠脑缺血再灌注损伤中的作用。方法以Homer1a基因敲除小鼠为研究对象,复制脑缺血再灌注小鼠模型,根据行为学评分标准评价小鼠脑缺血再灌注的损伤程度。运用TTC染色法观察计算小鼠脑梗死体积。Tunel法检测脑缺血再灌注小鼠脑组织中细胞凋亡情况。GFAP法检测星形胶质细胞的增殖情况。Western blot检测小鼠脑组织中IL-1β、TNF-α、IL-6水平。结果实验组小鼠行为学评分高于脑缺血组,说明Homer 1a基因敲除小鼠脑缺血再灌注损伤较脑缺血组更为严重。实验组小鼠脑梗死体积与脑缺血组比较,差异有统计学意义(P<0.05),实验组增多。实验组小鼠脑组织细胞凋亡高于脑缺血组(P<0.05)。实验组和脑缺血组小鼠脑组织中星形胶质细胞数都高于正常组(P<0.05),而实验组小鼠脑组织中星型胶质细胞数量低于脑缺血组。实验组小鼠脑组织中IL-1β、TNF-α、IL-6表达水平均高于脑缺血组(P<0.05)。结论 Homer 1a基因敲除小鼠可以加重脑缺血再灌注后的损伤。Homer 1a可以减弱脑缺血再灌注损伤。 展开更多
关键词 脑缺血 Homer1a 星形胶质细胞 基因敲除
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在中国澳门吸烟人群中的肺癌筛查研究 被引量:10
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作者 张晓战 张振荣 +6 位作者 程鲲 杨贞勇 朱伟国 周伟文 林肇聪 曹亚兵 李牧 《中国肺癌杂志》 CAS CSCD 北大核心 2021年第8期548-556,共9页
背景与目的中国澳门肺癌发病率逐年上升,吸烟人群是肺癌的高发人群,本研究旨在了解中国澳门长期吸烟人群的肺癌发病情况及胸部低剂量计算机断层扫描(low-dose computed tomography,LDCT)肺结节特点。方法通过澳门中华医学会会员私家医... 背景与目的中国澳门肺癌发病率逐年上升,吸烟人群是肺癌的高发人群,本研究旨在了解中国澳门长期吸烟人群的肺癌发病情况及胸部低剂量计算机断层扫描(low-dose computed tomography,LDCT)肺结节特点。方法通过澳门中华医学会会员私家医生推荐及宣传招募中国澳门无症状长期吸烟人士,行胸部LDCT检查,分析肺癌、肺部结节检出率及影像学特点。结果符合纳入条件者291例,检出肺癌10例,检出率3.44%(95%CI:2.78%-4.01%),其中,肺腺癌5例,鳞癌、小细胞肺癌各2例,腺鳞癌1例。早期肺癌4例,占40%。212例检出肺结节,肺结节总检出率72.9%(95%CI:67.8%-78.0%);疑似肺癌结节44例,检出率15.1%(95%CI:11.0%-19.2%)。单发结节51例,无肺癌检出;多发结节161例,检出肺癌9例,两组肺癌检出率无统计学差异(P>0.05)。<6 mm实性结节与<5 mm非实性结节组168例,未检出肺癌;≥6 mm实性结节与≥5 mm非实性结节组44例,检出肺癌9例,两组比较有统计学差异(P<0.05)。结论长期吸烟人群中肺癌检出率高,类型以腺癌为主,肺部结节发生率高,当实性结节≥6 mm或非实性结节≥5 mm时,肺癌检出率增高。建议在符合高危因素的男性吸烟人群中推行胸部LDCT筛查肺癌,女性肺癌筛查,应重新界定高危因素。 展开更多
关键词 肺肿瘤 筛查 早期诊断
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2型糖尿病患者血清SUA和CysC水平与糖尿病视网膜病变的关系 被引量:3
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作者 程坤 谢金凤 +2 位作者 唐刚学 黄前伟 黄胜 《国际眼科杂志》 CAS 北大核心 2022年第11期1867-1871,共5页
目的:探讨血清尿酸(SUA)、胱抑素C(CysC)水平与糖尿病视网膜病变(DR)的关系。方法:前瞻性研究。选取2019-05/2021-05本院收治无DR的2型糖尿病(T2DM)患者53例和DR患者83例,DR患者中包括非增殖型糖尿病视网膜病变(NPDR)47例、增殖型糖尿... 目的:探讨血清尿酸(SUA)、胱抑素C(CysC)水平与糖尿病视网膜病变(DR)的关系。方法:前瞻性研究。选取2019-05/2021-05本院收治无DR的2型糖尿病(T2DM)患者53例和DR患者83例,DR患者中包括非增殖型糖尿病视网膜病变(NPDR)47例、增殖型糖尿病视网膜病变(PDR)36例。另选取同期体检中心体检健康者48人作为对照组。比较受试者血清学指标,尿酸氧化酶法检测SUA水平,免疫比浊法检测血清CysC含量,Spearman相关性分析血清SUA、CysC与其他血清学指标的相关性,多因素线性逐步回归法分析血清SUA、CysC的影响因素,使用受试者工作特征曲线(ROC)分析血清SUA、CysC对DR预测效能。结果:T2DM组、NPDR组和PDR组的体质量指数(BMI)、收缩压(SBP)均明显高于对照组(均P<0.05),PDR组的SBP均明显高于T2DM组和NPDR组(均P<0.05),NPDR组和PDR组糖尿病病程均明显高于T2DM组(均P<0.05),PDR组糖尿病病程明显高于NPDR组(P<0.05)。对照组、T2DM组、NPDR组、PDR组纳入对象中空腹血糖(FPG)、糖化血红蛋白(HbA1c)、SUA、CysC水平呈逐渐明显升高趋势(均P<0.001),PDR组的低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)水平明显高于对照组(均P<0.05),而高密度脂蛋白胆固醇(HDL-C)水平明显低于对照组(P<0.05)。血清SUA水平与FPG、HbA1c、TC、TG水平呈正相关(r_(s)=0.564、0.631、0.513、0.408,P<0.001),与HDL-C、LDL-C无相关性(r_(s)=-0.061、0.035,P>0.05);血清CysC水平与FPG、HbA1c、TC、TG水平呈正相关(r_(s)=0.524、0.692、0.395、0.435,P<0.001),与HDL-C、LDL-C无相关性(r_(s)=-0.012、0.049,P>0.05),FPG、HbA1c、TC、TG是血清SUA、CysC水平影响因素(P<0.001)。SUA、CysC联合检测时曲线下面积(AUC)(0.892,95%CI:0.840~0.944,敏感性71.1%,特异性94.3%)显著高于其单独检测AUC[SUA(0.807,95%CI:0.735~0.879,敏感性69.9%,特异性75.5%)、CysC(0.763,95%CI:0.684~0.841,敏感性69.9%,特异性75.5%)](均P<0.05)。结论:随着DR病情严重程度加重而血清SUA、CysC水平逐渐升高。血清SUA、CysC联合检测可提高DR诊断预测效能。 展开更多
关键词 2型糖尿病 糖尿病视网膜病变 血清尿酸(SUA) 胱抑素C(CysC) 临床意义
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SIGN-CHANGING SOLUTIONS FOR THE STATIONARY KIRCHHOFF PROBLEMS INVOLVING THE FRACTIONAL LAPLACIAN IN R^N 被引量:4
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作者 kun cheng Qi GAO 《Acta Mathematica Scientia》 SCIE CSCD 2018年第6期1712-1730,共19页
In this paper, we study the existence of least energy sign-changing solutions for aKirchhoff-type problem involving the fractional Laplacian operator. By using the constraintvariation method and quantitative deformati... In this paper, we study the existence of least energy sign-changing solutions for aKirchhoff-type problem involving the fractional Laplacian operator. By using the constraintvariation method and quantitative deformation lemma, we obtain a least energy nodal solu-tion ub for the given problem. Moreover, we show that the energy of ub is strictly larger thantwice the ground state energy. We also give a convergence property of ub as b O, where bis regarded as a positive parameter. 展开更多
关键词 Kirchhoff equation fractional Laplaciau sign-changing solutions
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In situ constructing of ultrastable ceramic@graphene core-shell architectures as advanced metal catalyst supports toward oxygen reduction 被引量:1
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作者 HuiWu Tao Peng +5 位作者 Zongkui Kou kun cheng Jie Zhang Jian Zhang Tian Meng Shichun Mu 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2017年第6期1160-1167,共8页
The changeable structure of 2 D graphene nanosheets makes the Pt-based nanoparticles(NPs) possess a low efficiency toward oxygen reduction reaction(ORR) and a short lifetime for proton exchange membrane fuel cells... The changeable structure of 2 D graphene nanosheets makes the Pt-based nanoparticles(NPs) possess a low efficiency toward oxygen reduction reaction(ORR) and a short lifetime for proton exchange membrane fuel cells. Thus, a unique Ti C@graphene core-shell structure material with low surface energy is designed and prepared by an in situ forming strategy, and firstly applied as a stable support of Pt NPs.The as-prepared Pt/GNS@Ti C catalyst presents a high activity. Especially, its ORR stability is remarkably improved. Even after 15000 potential cycles, the half-wave potential and mass activity toward ORR have almost no change. This can be attributed to that the graphene nanosheet existing in a sphere shape effectively avoids the restacking or folding caused by the giant surface tension in 2 D graphene nanosheets,impeding the decrease of the triple-phase boundary on Pt NPs. Significantly, the power density of fuel cells with our novel catalyst reaches 853 m V cmunder a low Pt loading(0.25 mg Pt cm) and H/Air conditions. These indicate the new ceramic@graphene core-shell nanocomposite is a promising application in fuel cells and other fields. 展开更多
关键词 GRAPHENE CORE-SHELL Oxygen reduction ELECTROCATALYST Stability PEM fuel cell
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THE MULTIPLICITY AND CONCENTRATION OF POSITIVE SOLUTIONS FOR THE KIRCHHOFF-CHOQUARD EQUATION WITH MAGNETIC FIELDS
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作者 Li WANG kun cheng Jixiu WANG 《Acta Mathematica Scientia》 SCIE CSCD 2022年第4期1453-1484,共32页
In this paper,we study the multiplicity and concentration of positive solutions for the following fractional Kirchhoff-Choquard equation with magnetic fields:(aε^(2s)+bε^(4 s-3)[u]_(ε)^(2),A/ε)(-Δ)_(A/ε)^(s)u+V(... In this paper,we study the multiplicity and concentration of positive solutions for the following fractional Kirchhoff-Choquard equation with magnetic fields:(aε^(2s)+bε^(4 s-3)[u]_(ε)^(2),A/ε)(-Δ)_(A/ε)^(s)u+V(x)u=ε^(-α)(Iα*F(|u|^(2)))f(|u|^(2))u in R^(3).Hereε>0 is a small parameter,a,b>0 are constants,s E(0,1),(-Δ)As is the fractional magnetic Laplacian,A:R^(3)→R^(3) is a smooth magnetic potential,Iα=Γ(3-α/2)/2απ3/2Γ(α/2)·1/|x|^(α) is the Riesz potential,the potential V is a positive continuous function having a local minimum,and f:R→R is a C^(1) subcritical nonlinearity.Under some proper assumptions regarding V and f,we show the multiplicity and concentration of positive solutions with the topology of the set M:={x∈R^(3):V(x)=inf V}by applying the penalization method and LjusternikSchnirelmann theory for the above equation. 展开更多
关键词 Fractional Kirchhoff-Choquard problem penalization method Ljusternik-Schnirelmann theory variational methods
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Insights into optimizing exosome therapies for acute skin wound healing and other tissue repair
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作者 Tianjing Sun Mo Li +6 位作者 Qi Liu Anyong Yu kun cheng Jianxing Ma Sean Murphy Patrick Michael McNutt Yuanyuan Zhang 《Frontiers of Medicine》 SCIE CSCD 2024年第2期258-284,共27页
Exosome therapy holds great promise as a novel approach to improve acute skin wound healing.This review provides a comprehensive overview of the current understanding of exosome biology and its potential applications ... Exosome therapy holds great promise as a novel approach to improve acute skin wound healing.This review provides a comprehensive overview of the current understanding of exosome biology and its potential applications in acute skin wound healing and beyond.Exosomes,small extracellular vesicles secreted by various stem cells,have emerged as potent mediators of intercellular communication and tissue repair.One advantage of exosome therapy is its ability to avoid potential risks associated with stem cell therapy,such as immune rejection or stem cells differentiating into unwanted cell types.However,further research is necessary to optimize exosome therapy,not only in the areas of exosome isolation,characterization,and engineering,but also in determining the optimal dose,timing,administration,and frequency of exosome therapy.Thus,optimization of exosome therapy is critical for the development of more effective and safer exosome-based therapies for acute skin wound healing and other diseases induced by cancer,ischemia,or inflammation.This review provides valuable insights into the potential of exosome therapy and highlights the need for further research to optimize exosome therapy for clinical use. 展开更多
关键词 EXOSOMES stem cells therapeutic impact SKIN wound healing
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Green manure rotation and application increase rice yield and soil carbon in the Yangtze River valley of China
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作者 Qian YUE Jianfei SUN +7 位作者 Jonathan HILLIER Jing SHENG Zhi GUO Puping ZHU kun cheng Genxing PAN Yunpeng LI Xin WANG 《Pedosphere》 SCIE CAS CSCD 2023年第4期589-599,共11页
The addition of organic matter via green manure rotation with rice is considered a smart agricultural practice to maintain soil productivity and support environmental sustainability.However,few studies have quantitati... The addition of organic matter via green manure rotation with rice is considered a smart agricultural practice to maintain soil productivity and support environmental sustainability.However,few studies have quantitatively assessed the impact of green manure rotation and application on the interactions between agronomic management practice,soil fertility,and crop production.In this study,800 pairs of data from 108 studies conducted in the agricultural region of the Yangtze River,China were assessed,and random forest(RF)modeling was performed to evaluate the effect of green manure rotation and application on rice yield and soil properties.Compared to a winter fallow system,rotation and application of green manure significantly increased rice yield and soil organic carbon(SOC)by 8.1%and 8.4%,respectively.According to the RF models,rice type,green manure application rate and duration,mineral and organic nitrogen application rates,and initial SOC content and soil pH were identified as the main drivers for rice yield and SOC changes.Marginal benefit analysis revealed that green manure application rates for early rice in double cropping system and the rice in single cropping system were approximately 20 and 26 t ha-1(fresh weight),respectively.Further,the optimum green manure application rate was approximately 25 t ha-1(fresh weight)for carbon sequestration.However,it should be noted that green manure application to soils with high SOC level might result in the soils becoming a net carbon source.Our study contributed scientific and quantitative indicators for achieving the greatest benefits in rice yield and increasing SOC upon application of green manure. 展开更多
关键词 carbon sequestration crop production random forest model soil fertility soil organic carbon
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Tumor-associated macrophages in liver cancer:From mechanisms to therapy 被引量:44
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作者 kun cheng Ning Cai +3 位作者 Jinghan Zhu Xing Yang Huifang Liang Wanguang Zhang 《Cancer Communications》 SCIE 2022年第11期1112-1140,共29页
Multidimensional analyses have demonstrated the presence of a unique tumor microenvironment(TME)in liver cancer.Tumor-associated macrophages(TAMs)are among the most abundant immune cells infiltrating the TME and are p... Multidimensional analyses have demonstrated the presence of a unique tumor microenvironment(TME)in liver cancer.Tumor-associated macrophages(TAMs)are among the most abundant immune cells infiltrating the TME and are present at all stages of liver cancer progression,and targeting TAMs has become one of the most favored immunotherapy strategies.In addition,macrophages and liver cancer cells have distinct origins.At the early stage of liver cancer,macrophages can provide a niche for the maintenance of liver cancer stem cells.In contrast,cancer stem cells(CSCs)or poorly differentiated tumor cells are key factors modulating macrophage activation.In the present review,we first propose the origin connection between precursor macrophages and liver cancer cells.Macrophages undergo dynamic phenotypic transition during carcinogenesis.In this course of such transition,it is critical to determine the appropriate timing for therapy and block specific markers to suppress protumoral TAMs.The present review provides a more detailed discussion of transition trends of such surface markers than previous reviews.Complex crosstalk occurs between TAMs and liver cancer cells.TAMs play indispensable roles in tumor progression,angiogenesis,and autophagy due to their heterogeneity and robust plasticity.In addition,macrophages in the TME interact with other immune cells by directing cell-to-cell contact or secreting various effector molecules.Similarly,tumor cells combined with other immune cells can drive macrophage recruitment and polarization.Despite the latest achievements and the advancements in treatment strategies following TAMs studies,comprehensive discussions on the communication between macrophages and cancer cells or immune cells in liver cancer are currently lacking.In this review,we discussed the interactions between TAMs and liver cancer cells(from cell origin to maturation),the latest therapeutic strategies(including chimeric antigen receptor macrophages),and critical clinical trials for hepatocellular carcinoma(HCC)and intrahepatic cholangiocar-cinoma(iCCA)to provide a rationale for further clinical investigation of TAMs as a potential target for treating patients with liver cancer. 展开更多
关键词 hepatocellular carcinoma intrahepatic cholangiocarcinoma tumor-associated macrophages IMMUNOTHERAPY
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Assessment of prophylactic antibiotics administration for acute pancreatitis:a meta-analysis of randomized controlled trials 被引量:6
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作者 Nan Ding Yong-Hui Sun +5 位作者 Li-Mei Wen Jian-Hua Wang Jian-Hua Yang kun cheng Hai Lin Qi-Long Chen 《Chinese Medical Journal》 SCIE CAS CSCD 2020年第2期212-220,共9页
Background:Recent evidence has shown that prophylactic antibiotic treatment in patients with acute pancreatitis is not associated with a significant decrease in mortality or morbidity.The use and efficacy of prophylac... Background:Recent evidence has shown that prophylactic antibiotic treatment in patients with acute pancreatitis is not associated with a significant decrease in mortality or morbidity.The use and efficacy of prophylactic antibiotic treatment in acute pancreatitis remain controversial.This meta-analysis was conducted to assess whether antibiotic prophylaxis is beneficial in patients with acute pancreatitis.Methods:We searched randomized controlled trials(RCTs)of prophylactic use of antibiotics using Medline(PubMed),Embase,the Cochrane Library,and Web of Science.The data were analyzed using Review Manager 5.3 software.We performed pooled analyses for infected pancreatic necrosis,mortality,surgical intervention,and non-pancreatic infection.Odds ratios(ORs)from each trial were pooled using a random or fixed effects model,depending on the heterogeneity of the included studies.Sub-group analysis or sensitivity analysis was conducted to explore potential sources of heterogeneity,when necessary.Results:Totally,11 RCTs involving 747 participants were included,with an intervention group(prophylactic use of antibiotics,n=376)and control group(n=371).No significant differences were found regarding antibiotic prophylaxis with respect to incidence of infected pancreatic necrosis(OR,0.74;95%confidence interval[CI],0.50–1.09;P=0.13),surgical intervention(OR,0.92;95%CI,0.62–1.38;P=0.70),and morality(OR,0.71;95%CI,0.44–1.15;P=0.16).However,antibiotic prophylaxis was associated with a statistically significant reduction in the incidence of non-pancreatic infection(OR,0.59;95%CI,0.42–0.84;P=0.004).Conclusions:Prophylactic antibiotics can reduce the incidence of non-pancreatic infection in patients with AP. 展开更多
关键词 ACUTE PANCREATITIS PROPHYLACTIC ADMINISTRATION ANTIBIOTICS META-ANALYSIS
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Comparative analysis of duodenum-preserving pancreatic head resection and pancreaticoduodenectomy 被引量:3
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作者 Yong-Hui Sun Nan Ding +3 位作者 kun cheng Hai Lin Jia-Qi Xu Qi-Long Chen 《Chinese Medical Journal》 SCIE CAS CSCD 2020年第17期2112-2113,共2页
There is controversy regarding the perioperative complications,safety,and long-term quality of life for duodenum-preserving pancreatic head resection(DPPHR)and pancreaticoduodenectomy(PD).We performed a comparative an... There is controversy regarding the perioperative complications,safety,and long-term quality of life for duodenum-preserving pancreatic head resection(DPPHR)and pancreaticoduodenectomy(PD).We performed a comparative analysis of DPPHR and PD in the treatment of benign and low-grade malignant diseases of the pancreatic head.The study was approved by the Institutional Review Board of the Ethics Committee of the First Affiliated Hospital of Xinjiang Medical University(No.K202001-14),and was in accordance with the Declaration of Helsinki.Because this was a retrospective study and the data analysis was performed anonymously,this study was exempt from informed consent from patients. 展开更多
关键词 DUODENUM PRESERVING RESECTION
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Silencing ofα-complex protein-2 reverses alcohol-and cytokine-induced fibrogenesis in hepatic stellate cells 被引量:2
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作者 Hao Liu Zhijin Chen +3 位作者 Wei Jin Ashutosh Barve Yu-Jui Yvonne Wan kun cheng 《Liver Research》 2017年第1期70-79,共10页
Background and aim:a-complex protein-2(aCP2)encoded by the poly(rC)binding protein 2(PCBP2)gene is responsible for the accumulation of type I collagen in fibrotic livers.In this study,we silenced the PCBP2 gene using ... Background and aim:a-complex protein-2(aCP2)encoded by the poly(rC)binding protein 2(PCBP2)gene is responsible for the accumulation of type I collagen in fibrotic livers.In this study,we silenced the PCBP2 gene using a small interfering RNA(siRNA)to reverse alcohol-and cytokine-induced profibrogenic effects on hepatic stellate cells(HSCs).Methods:Primary rat HSCs and the HSC-T6 cell line were used as fibrogenic models to mimic the initiation and perpetuation stages of fibrogenesis,respectively.We previously found that a PCBP2 siRNA,which efficiently silences expression of aCP2,reduces the stability of type I collagen mRNA.We investigated the effects of the PCBP2 siRNA on cell proliferation and migration.Expression of type I collagen in HSCs was analyzed by quantitative real-time PCR and western blotting.In addition,we evaluated the effects of the PCBP2 siRNA on apoptosis and the cell cycle.Results:PCBP2 siRNA reversed multiple alcohol-and cytokine-induced profibrogenic effects on primary rat HSCs and HSC-T6 cells.The PCBP2 siRNA also reversed alcohol-and cytokine-induced accumulation of type I collagen as well as cell proliferation and migration.Moreover,the combination of LY2109761,a transforming growth factor-b1 inhibitor,and the PCBP2 siRNA exerted a synergistic inhibitive effect on the accumulation of type I collagen in HSCs.Conclusions:Silencing of PCBP2 using siRNA could be a potential therapeutic strategy for alcoholic liver fibrosis. 展开更多
关键词 Hepatic stellate cells Primary HSC MYOFIBROBLAST Liver fibrosis Poly(rC)binding protein(PCBP)2 Transforming growth factor(TGF)-b Platelet-derived growth factor(PDGF) Epidermal growth factor(EGF) LY2a09761 Migration
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