Molecular characterization and functional expression of opioid receptor-libe_1 receptor

The opioid receptor-libel receptor (ORL), an orphan receptor whose human and murine complementary DNAs,has been characterized recently. ORL transcripts are particularly abundant in the central nervous system. We demonstrated that ORL expressed in human neuroblastoma SK-N-SH and SH-SY5Y cell lines by radioligand binding assay, reverse transcription polymerase chain reaction (RT-PCR) and Northern analysis in the present study. Stimulation with ORL1 specific agonist, nociceptin/orphanin Fo, increased [34S]GTPrγS binding to SK-N-SH cell membranes (EC50 = 14 ±0.45 nM), and attenuated forskolin-stimulated accumulation of cellular cAMP (EC50= 0.80 ±0.45 nM, indicative that activation of ORL1 activates G proteins and inhibits adenylyl cyclase. Activation of ORL1 receptor was also accessed using CHO:hORL1 cell line by microphysiometer. Treatment of nociceptin/orphanin FQ increased extracellular acidification rate significantly.

Polymorphism-Dependent Dynamic Ultralong Organic Phosphorescence

Developing ultralong organic phosphorescence(UOP)materials with smart response to external stimuli is of great interest in photonics applications,whereas the manipulation of molecular stacking on tuning such dynamic UOP is still a formidable challenge.Herein,we have reported two polymorphs with distinct photoactivated dynamic UOP behavior based on a pyridine derivative for the first time.Our experiment revealed that the dynamic UOP behavior including photoactivation and deactivation feature is highly dependent on irradiation intensity and environmental atmosphere.Additionally,given the unique dynamic UOP feature,these phosphors have been successfully applied to phosphorescence-dependent molecular logic gate and timing data storage.This result not only paves a way to design smart functional materials but also expands the scope of the applications on organic phosphorescence materials.

Zircon U-Pb geochronology of the Chinese continental crust: a preliminary analysis of the Elsevier science database

The amount of zircon U-Pb geochronological data for China has grown rapidly in recent years.Nearly 410,000 items of zircon U-Pb geochronological data,representing more than 7,000 relevant articles in the Elsevier Science Database,have been collected to a database in this research.Statistics on the ages and absolute errors of these collated data,yielded smallest standard errors for(206Pb/238U),(207Pb/235U),and(207Pb/206Pb)ages within respective time intervals of<1388.96 Ma,1388.96–3282.52 Ma,and>3282.52 Ma.The ages and their absolute errors were determined using three main geochronology methods,based on laser ablation inductively coupled mass spectrometer(LA-ICP-MS),sensitive high-resolution ion microprobe(SHRIMP),and secondary ion mass spectrometer(SIMS)measurements.We compared the influence of these different methods on errors for each age interval.In addition,using a Gaussian model of multi-peak fitting of zircon U-Pb age frequencies,we identified seven growth peaks in zircons from the Chinese continental crust,which are 48.60 Ma,131.49 Ma,249.91 Ma,444.27 Ma,835.95 Ma,1860.65 Ma,and 2505.54 Ma.It is clear that there are correspondences between these ages and various geological events,namely,the Wutai movement,Lvliang movement,Jinning movement,Caledonian movement,Indo-China movement,Yanshan movement,and Himalayaorogeny movement,respectively.The time and spatial distributions of these zircons correspond to distinct geological events on the Chinese continent,reflecting its crustal evolution.

Numerical Studies of Estimation of Fracture Parameters for Mismatched Welded Joints with HAZ Cracks

An idealized tri-material assumption is established to describe the constitutive relationship of mismatched welded joints by considering the influence of heat affected zone（HAZ）.The fracture parameters Jand C＊are estimated for mismatched welded joints with HAZ cracks by finite element analysis with ABAQUS.A middle crack tension（M（T））specimen is utilized in the analysis for different material properties and geometries of the weldment.The influence of mechanical property and geometry on the fracture parameters Jand C＊of the specimen is discussed for the welded joints with HAZ crack.The results suggest that the HAZ property is a significant factor in the estimation of Jand C＊for the mismatched welded joint with HAZ crack.

Subtle structure tailoring of metal-free triazine luminogens for highly efficient ultralong organic phosphorescence

Ultralong organic phosphorescent materials have invoked considerable attention for their great potential in sensing,data encryption,information anti-counterfeiting and so forth.However,effective ways to achieve highly efficient ultralong organic phosphorescence(UOP)in metal-free organic materials remain a great challenge.Herein,we designed three isomers based on asymmetric triazines with various bromine substituted positions.Impressively,phosphorescence efficiency of p-BrAT in solid state can reach up to 9.7%with a long lifetime of 386 ms,which was one of the highest efficient UOP materials reported so far.Theoretical calculations further demonstrated that para-substitution exhibited the most effective radiative transition for triplet excitons.These results will provide an effective approach to achieving highly efficient UOP materials.

Crustal evolution events in the Chinese continent: evidence from a zircon U-Pb database

The zircon U-Pb chronology database provides a good opportunity to obtain important zircon growth peak periods in the Earth’s history so as to study the origin and evolution of the crust.It should be noted that research preference affects the objectivity of zircon sampling,leading to hot data in the database and age statistics.To evaluate the influence of hot data on statistical results,the W and Y indexes are introduced.Using a Gaussian model of multipeak fitting of zircon U-Pb age frequencies,we identify seven major growth peaks in zircons from the Chinese continental crust,which are 2498.95,1855.82,828.88,444.29,249.46,131.96,and 58.21 Ma.Due to differences in the time scales of zircon growth peaks,these peaks can be divided into two categories:first-order zircon growth peaks(Ⅰ)and second-order zircon growth peaks(Ⅱ),which represent longer and shorter time scales,perhaps due to different kinds of geological dynamics,respectively.In addition,there are clear correspondences between these ages and various geological events recognized by most scholars,namely,the Wutai orogeny,Lvliang orogeny,Jinning orogeny,Caledonian orogeny,Indosinian orogeny,Yanshanian orogeny,and Himalayan orogeny,respectively.