Ultra high-throughput DNA sequencing has been a very hot topic beyond the field of genomic researches.There have been various approaches to this issue ranging from direct observation of individual DNA synthesis[1],amp...Ultra high-throughput DNA sequencing has been a very hot topic beyond the field of genomic researches.There have been various approaches to this issue ranging from direct observation of individual DNA synthesis[1],amperometric/optical detection of bases using a nanopore[2,3]and direct sequencing with a high-resolution probe microscope[4].Recently,we have developed a technique to chemically modify all nucleobases in DNA[5],so that the bases A,T,G,C can be differentiated in the sequence by using high-resolution electron microscopy(EM).展开更多
文摘Ultra high-throughput DNA sequencing has been a very hot topic beyond the field of genomic researches.There have been various approaches to this issue ranging from direct observation of individual DNA synthesis[1],amperometric/optical detection of bases using a nanopore[2,3]and direct sequencing with a high-resolution probe microscope[4].Recently,we have developed a technique to chemically modify all nucleobases in DNA[5],so that the bases A,T,G,C can be differentiated in the sequence by using high-resolution electron microscopy(EM).
