Coenzyme Q10 is widely used in food,cosmetics and pharmaceuticals,possessing a broad market.Rhodobacter sphaeroides is enriched in natural coenzyme Q10 and is becoming an important microorganism for producing natural ...Coenzyme Q10 is widely used in food,cosmetics and pharmaceuticals,possessing a broad market.Rhodobacter sphaeroides is enriched in natural coenzyme Q10 and is becoming an important microorganism for producing natural coenzyme Q10.The paper reviewed the biosynthesis pathways of coenzyme Q10 in R.sphaeroides and the advances in enhancement of coenzyme Q10 production in R.sphaeroides based on metabolic engineering.展开更多
In the present study,single factors including inoculation amount,fermentation temperature,fermentation duration and ratio of fermentation medium volume to total flask volume were optimized for enhancing the production...In the present study,single factors including inoculation amount,fermentation temperature,fermentation duration and ratio of fermentation medium volume to total flask volume were optimized for enhancing the production of coenzyme Q10 from genetic engineered Rhodobacter sphaeroides overexpressing UbiE.The experimental results suggested that optimal single factors were: inoculation amount 2%,fermentation temperature 30 ℃,fermentation duration 48 h,and fermentation medium volume to total flask volume 80%.The present study will promote the large scale production of CoQ10 from microorganisms.展开更多
基金Supported by Talent Project of Sichuan University of Science&Engineering(2015RC27)Meat Processing Key Laboratory of Sichuan Province(16R-27)
文摘Coenzyme Q10 is widely used in food,cosmetics and pharmaceuticals,possessing a broad market.Rhodobacter sphaeroides is enriched in natural coenzyme Q10 and is becoming an important microorganism for producing natural coenzyme Q10.The paper reviewed the biosynthesis pathways of coenzyme Q10 in R.sphaeroides and the advances in enhancement of coenzyme Q10 production in R.sphaeroides based on metabolic engineering.
基金Supported by the Research Project of Sichuan University of Science&Engineering(2015RC27)Scientific Research Foundation of the Education Department of Sichuan Province(15ZA0222)Research Project of Meat Processing Key Laboratory of Sichuan Province(16R-27)
文摘In the present study,single factors including inoculation amount,fermentation temperature,fermentation duration and ratio of fermentation medium volume to total flask volume were optimized for enhancing the production of coenzyme Q10 from genetic engineered Rhodobacter sphaeroides overexpressing UbiE.The experimental results suggested that optimal single factors were: inoculation amount 2%,fermentation temperature 30 ℃,fermentation duration 48 h,and fermentation medium volume to total flask volume 80%.The present study will promote the large scale production of CoQ10 from microorganisms.
