The development of organs-on-a-chip has resulted in advances in the reconstruction of 3D cellular microenvironments.However,there remain limitations regarding applicability and manufacturability.Here,we present an inj...The development of organs-on-a-chip has resulted in advances in the reconstruction of 3D cellular microenvironments.However,there remain limitations regarding applicability and manufacturability.Here,we present an injection-molded plastic array 3D universal culture platform(U-IMPACT)for various biological applications in a single platform,such as cocultures of various cell types,and spheroids(e.g.,tumor spheroids,neurospheres)and tissues(e.g.,microvessels).The U-IMPACT consists of three channels and a spheroid zone with a 96-well plate form factor.Specifically,organoids or spheroids(~500μm)can be located in designated areas,while cell suspensions or cellladen hydrogels can be selectively placed in three channels.For stable multichannel patterning,we developed a new patterning method based on capillary action,utilizing capillary channels and the native contact angle of the materials without any modification.We derived the optimal material hydrophilicity(contact angle of the body,45–90°;substrate,<30°)for robust patterning through experiments and theoretical calculations.We demonstrated that the U-IMPACT can implement 3D tumor microenvironments for angiogenesis,vascularization,and tumor cell migration.Furthermore,we cultured neurospheres from induced neural stem cells.The U-IMPACT can serve as a multifunctional organ-on-achip platform for high-content and high-throughput screening.展开更多
基金supported by the National Research Foundation of Korea(NRF,Grant No.NRF-2021R1A3B1077481)。
文摘The development of organs-on-a-chip has resulted in advances in the reconstruction of 3D cellular microenvironments.However,there remain limitations regarding applicability and manufacturability.Here,we present an injection-molded plastic array 3D universal culture platform(U-IMPACT)for various biological applications in a single platform,such as cocultures of various cell types,and spheroids(e.g.,tumor spheroids,neurospheres)and tissues(e.g.,microvessels).The U-IMPACT consists of three channels and a spheroid zone with a 96-well plate form factor.Specifically,organoids or spheroids(~500μm)can be located in designated areas,while cell suspensions or cellladen hydrogels can be selectively placed in three channels.For stable multichannel patterning,we developed a new patterning method based on capillary action,utilizing capillary channels and the native contact angle of the materials without any modification.We derived the optimal material hydrophilicity(contact angle of the body,45–90°;substrate,<30°)for robust patterning through experiments and theoretical calculations.We demonstrated that the U-IMPACT can implement 3D tumor microenvironments for angiogenesis,vascularization,and tumor cell migration.Furthermore,we cultured neurospheres from induced neural stem cells.The U-IMPACT can serve as a multifunctional organ-on-achip platform for high-content and high-throughput screening.
