High-resolution liquid chromatography separation is essential to in-depth proteomic profiling of complex biological samples.Herein,we established an ion-pair reversed-phase×reversed-phase two-dimensional liquid c...High-resolution liquid chromatography separation is essential to in-depth proteomic profiling of complex biological samples.Herein,we established an ion-pair reversed-phase×reversed-phase two-dimensional liquid chromatography(IPRP×RP 2DLC)strategy for comprehensive proteomic analysis.Both RPLC separation dimensions were performed at low pH,with trifluoroacetic acid(TFA)and formic acid(FA)as mobile phase addictive,respectively.As the good separation resolution offered by ion-pairing effect of TFA,the fractionation efficiency was greatly improved with 74.0%peptides identified in just one fraction.Comparing with conventional high pH RP fractionation,the overall separation rate of IPRP was about 1.6 times that of high-pH RP,which increased the number of identified peptides by 21%.Further,2169 proteins and 8540 peptides were confidently identified from crude serum sample by our IPRP×RP 2DLC strategy,exhibiting great potential in clinical proteomics in the future.展开更多
目的应用MR多回波Dixon定量技术,探讨肥胖儿童肾脏脂肪异常沉积与早期肾损伤之间的关系。方法共纳入研究对象74例,其中正常健康儿童20名(对照组);肥胖组54例分为尿蛋白正常组和尿微量蛋白异常组。所有研究对象均在Philips 1.5 T MR行肾...目的应用MR多回波Dixon定量技术,探讨肥胖儿童肾脏脂肪异常沉积与早期肾损伤之间的关系。方法共纳入研究对象74例,其中正常健康儿童20名(对照组);肥胖组54例分为尿蛋白正常组和尿微量蛋白异常组。所有研究对象均在Philips 1.5 T MR行肾脏mDixon-Quant检查,通过绘制感兴趣区(ROI)测量肾脏脂肪分数。分析双肾脂肪分数差异及其与体重指数(BMI)的相关性,比较各组之间肾脏脂肪分数的差异,分析其与尿微量蛋白间的相关性。结果74例研究对象双侧肾脏测量的脂肪分数无明显统计学差异(t=0.633,P=0.529)。尿微量蛋白异常组肾脏脂肪分数显著高于尿蛋白正常组及对照组(P<0.001)。BMI与肾脏脂肪分数呈中度正相关(r=0.569,P<0.001)。在校正了潜在的混杂因素后,肾脏脂肪分数和尿微量蛋白之间的关系仍然具有统计学意义,尤其与尿微量白蛋白及尿免疫球蛋白G呈中高度相关(r=0.636,P<0.001;r=0.579,P<0.001)。结论无创性mDixon-Quant磁共振成像技术可准确定量肥胖儿童肾脏脂肪含量,肾脏中的脂肪沉积含量与早期肾损伤相关。展开更多
基金National Key R&D Program of China(No.2019YFE0119300)National Natural Science Foundation of China(No.32088101)+1 种基金Original Innovation Project of CAS(No.ZDBS-LY-SLH032)Project of Dalian Institute of Chemical Physics,CAS,China(No.DICPI202007).
文摘High-resolution liquid chromatography separation is essential to in-depth proteomic profiling of complex biological samples.Herein,we established an ion-pair reversed-phase×reversed-phase two-dimensional liquid chromatography(IPRP×RP 2DLC)strategy for comprehensive proteomic analysis.Both RPLC separation dimensions were performed at low pH,with trifluoroacetic acid(TFA)and formic acid(FA)as mobile phase addictive,respectively.As the good separation resolution offered by ion-pairing effect of TFA,the fractionation efficiency was greatly improved with 74.0%peptides identified in just one fraction.Comparing with conventional high pH RP fractionation,the overall separation rate of IPRP was about 1.6 times that of high-pH RP,which increased the number of identified peptides by 21%.Further,2169 proteins and 8540 peptides were confidently identified from crude serum sample by our IPRP×RP 2DLC strategy,exhibiting great potential in clinical proteomics in the future.
文摘目的应用MR多回波Dixon定量技术,探讨肥胖儿童肾脏脂肪异常沉积与早期肾损伤之间的关系。方法共纳入研究对象74例,其中正常健康儿童20名(对照组);肥胖组54例分为尿蛋白正常组和尿微量蛋白异常组。所有研究对象均在Philips 1.5 T MR行肾脏mDixon-Quant检查,通过绘制感兴趣区(ROI)测量肾脏脂肪分数。分析双肾脂肪分数差异及其与体重指数(BMI)的相关性,比较各组之间肾脏脂肪分数的差异,分析其与尿微量蛋白间的相关性。结果74例研究对象双侧肾脏测量的脂肪分数无明显统计学差异(t=0.633,P=0.529)。尿微量蛋白异常组肾脏脂肪分数显著高于尿蛋白正常组及对照组(P<0.001)。BMI与肾脏脂肪分数呈中度正相关(r=0.569,P<0.001)。在校正了潜在的混杂因素后,肾脏脂肪分数和尿微量蛋白之间的关系仍然具有统计学意义,尤其与尿微量白蛋白及尿免疫球蛋白G呈中高度相关(r=0.636,P<0.001;r=0.579,P<0.001)。结论无创性mDixon-Quant磁共振成像技术可准确定量肥胖儿童肾脏脂肪含量,肾脏中的脂肪沉积含量与早期肾损伤相关。