重组人血管内皮抑制素（恩度）联合化疗的不良反应自动监测研究

目的:对住院患者使用重组人血管内皮抑制素注射液(恩度)联合化疗的相关毒副反应进行自动监测研究,为临床合理安全使用该药提供药品风险参考。方法:借助“军队医疗机构药品不良反应事件主动监测与智能评估警示系统”(ADEASAS),调取2006年1月–2017年12月使用重组人血管内皮抑制素注射液且符合纳入标准的住院患者,对该药联合化疗的相关不良反应进行ADE主动监测,并结合人工评价进行回顾性分析。结果:共有3706例(4371例次)患者纳入研究,均使用联合重组人血管内皮抑制素注射液的化疗方案,联用的化疗药物主要包括铂类、烷化剂、抗代谢药等。按用药例次排序前三位科室依次是骨科(40.75%),肿瘤内科(34.45%)、介入放射科(13.64%)。4371例次患者中血小板减少、白细胞减少、肝损伤的发生率分别为18.20%、55.43%、9.76%,未见心脏毒性反应,与Meta分析结果基本相符;系统报警阳性率分别为89.63%、84.61%、94.41%。结论:重组人血管内皮抑制素注射液联合化疗已广泛应用于多种实体瘤的临床治疗中,该药联合化疗未加重化疗相关毒副作用,安全性相对较好,研究结果仍有待多中心、大样本的真实世界研究进一步验证。

基于中药系统鉴定法进行青葙子药材的鉴别研究

目的:利用中药系统鉴定法鉴别青葙子及其混伪品,为青葙子药材的准确鉴定及质量控制提供参考。方法:通过性状、微性状、显微鉴别和DNA条形码分析等方法,对青葙子药材及其混伪品进行比对分析,利用MEGA 7.0软件计算物种DNA条形码序列的种内、种间Kimura 2-Parameter遗传距离、评估序列的条形码间距,采用邻接法构建系统聚类树。结果:仅凭性状鉴别难以区分青葙子与混伪品;青葙子及其混伪品的微性状、显微特征差异明显,可作为其鉴定的重要依据之一;经筛选,推荐ITS序列作为青葙子及其混伪品的DNA条形码候选序列,trnL序列作为补充序列。结论:中药系统鉴定法能够完成对青葙子药材的精准鉴别。

中药材青葙子PCR鉴别方法研究

目的:建立一种能特异性检测青葙子及其混伪品的分子鉴定方法。方法:针对青葙子SRAP标记筛选并设计特异性引物,各地采集青葙子、鸡冠花子、苋菜子、刺苋子和反枝苋子样品,提取DNA作为模板,建立并优化PCR反应条件,利用特异性引物对不同产地的青葙子及其混伪品进行扩增和电泳检测,根据特异性条带的大小及有无进行真伪及掺伪鉴别,并对其检出限、耐受性和适用性进行考察与验证。结果:退火温度为60℃、循环次数为30次时,青葙子、鸡冠花子分别在206和581 bp处出现单一明亮条带,其他混伪品及空白对照均无此条带;该方法对青葙子药材DNA的最低检测限为1 ng;对于青葙子中掺杂鸡冠花子的检出限是2%。结论:建立的特异性PCR方法可准确鉴定青葙子及其混伪品。

基于ITS2序列位点特异性PCR鉴别广藿香及其混伪品

目的建立一种简便、准确鉴别广藿香及其混伪品的方法。方法通过对GenBank数据库收录的广藿香及其混伪品的ITS2序列进行对比分析,根据差异位点设计位点特异性鉴别引物,并优化PCR条件,对广藿香新鲜植物、干燥药材、含广藿香中成药及其混伪品进行扩增和检测。结果广藿香新鲜植物、干燥药材及含广藿香中成药均扩增出162 bp条带,而广藿香的混伪品均无条带,且该方法灵敏度高,检出限可达0.3 g·L^(-1)。结论所建立的位点特异性PCR方法可实现广藿香的准确鉴别,具有操作简便、稳定可靠、应用范围广的优点。

广藿香分子生药学研究进展

分子生药学是生药学与分子生物学有机融合形成的新兴交叉学科,是一门在分子水平研究生药的分类与鉴定、栽培与保护及有效成分生产的科学。广藿香作为道地南药的研究热点,其在分子生药学方面的研究也一直受到学者们的关注,并在不断探索中取得了一定成果。该文综述了近年来广藿香的分子生药学相关研究,并对该领域进行了展望,以期为广藿香资源的保护、开发和利用提供参考。

Rapid identification of Dendrobium officinale using Loop-Mediated Isothermal Amplification (LAMP) method

Dendrobium officinale is not only an ornamental plant, but also a valuable medicinal herb that is widely used in traditional Chinese medicine. However, distinguishing D. officinale from other Dendrobium species is usually a difficult task. In this study,we developed a rapid identification protocol for D. officinale using the loop-mediated isothermal amplification(LAMP) method. A set of primers were specifically designed to detect a modified internal transcribed spacer region of D. officinale at 65 ℃ within 40 min after adding SYBR~? Green I, which was used for the detection of D. officinale. Unlike commonly used adulterants, reaction mixtures containing D. officinale DNA changed from orange to green, and this color change was easily observed with the naked eye. Thus, this methodology can be used to accurately differentiate D. officinale from other Dendrobium species, is quick as all D. officinale samples were amplified within 40 min, and specific as samples of the adulterants were not amplified. The specificity of this LAMP-based method was confirmed by testing 17 samples of D. officinale and 32 adulterant samples from other Dendrobium species. This LAMP-based rapid identification method does not require expensive equipment or specialized techniques and can be used in field surveys for accurate and fast on-site identification.

The Anti-glioma Effect of Luteolin Via Mir-384/PIWIL4 Pathway and Its Influencing Mechanism

Objective:To explore the anti-glioma effect and influence of luteolin through mi R-384/PIWIL4 pathway.Methods:30 cases of human glioblastoma multiform U251,U87,A172,SHG44 cells,and 30 cases of the normal glial cell NHA were treated with luteolin.The control group was detected by Western blot,and the level of PIWIL4 in the cytoplasm and nucleus of each cell line was analyzed.The expression levels of PIWIL4 m RNA and mi R-384 in each cell line were detected by qRT-PCR.Results:the glial cell NHA with normal PIWIL4 level in the cytoplasm was related to U251,U87,A172,and SHG44 human pleomorphic glioma cells.The m RNA expression levels of U251,U87,A172,and SHG44 human pleomorphic glioma cells were significantly higher than those of normal gliomas(P<0.05),and the mi R-384 expression levels of U251,U87,A172,and SHG44 human pleomorphic glioma cells were significantly higher than those of normal gliomas(P<0.05).With the continuous increase of luteolin concentration,glioma cells showed a significant increase in dependence(P<0.05).Conclusion:Luteolin could up-regulate mi R-384,inhibit PIWIL4,and regulate the biological activity of glioma cell proliferation and migration.