Objective:To investigate the effects of Eriocitrin on the proliferation and migration of Lung adenocarcinoma(LUAD)cells A549 and H1299,and the mechanism of Epithelial-Mesenchymal Transition(EMT).Methods:The effects of...Objective:To investigate the effects of Eriocitrin on the proliferation and migration of Lung adenocarcinoma(LUAD)cells A549 and H1299,and the mechanism of Epithelial-Mesenchymal Transition(EMT).Methods:The effects of different Eriocitrin on the proliferation of LUAD cells A549 and H1299 were examined by CCK8 method.EMT-associated epithelial calmodulin(E-cadherin and N-cadherin),vimentin,ferroptosis-associated protein SLC7A11,GPX4,FTH were detected by Western Blot and expression of mRNA of EMT marker molecules E-cadherin,N-cadherin,Snail were detected by qRT-PCR.Effects of saccharomyces cerevisiae suberin on ferroptosis in LUAD cells as observed by lipid reactive oxygen species(ROS)assay.Results:Eriocitrin could significantly inhibit the proliferative behavior of LUAD cells A549 and H1299 and showed a certain dose-and time-dependence.Compared with the control group,different concentrations of Eriocitrin could significantly reduce the scratch healing rate after 24 and 48 h of action,and the difference was statistically significant(P<0.01).The expression of ROS is increased,EMT-related protein E-cadherin was increased in LUAD cells A549 and H1299 compared with the control group after the intervention with Eriocitrin.N-cadherin and Vimentin expression was decreased.E-cadherin mRNA expression was increased,and N-cadherin,Snail mRNA expression was decreased,expression of ferroptosis-associated protein SLC7A11,GPX4,FTH was decreased,the difference was statistically significant(P<0.05).Conclusion:Eriocitrin may inhibit the proliferation and migration of LUAD cells by regulating the EMT pathway and has potential application in LUAD prevention and adjuvant chemotherapy.展开更多
目的评价与比较序贯器官衰竭评分(SOFA)、Logistic脏器功能不全评分(LODS)、牛津急性疾病严重程度评分(OASIS)、简明急性生理功能评分Ⅱ(SAPSⅡ)、简明急性生理功能评分(SAPS)、全身炎症反应综合征(SIRS)和Elixhauser共病指数(ECI)评分...目的评价与比较序贯器官衰竭评分(SOFA)、Logistic脏器功能不全评分(LODS)、牛津急性疾病严重程度评分(OASIS)、简明急性生理功能评分Ⅱ(SAPSⅡ)、简明急性生理功能评分(SAPS)、全身炎症反应综合征(SIRS)和Elixhauser共病指数(ECI)评分系统对大肠埃希菌血流感染脓毒症患者住院病死率的预测能力。方法从MIMIC-Ⅲ(Medical Information Mart for Intensive Care)重症医学数据库中筛选出入重症监护病房(ICU)且早期血培养结果为大肠埃希菌的成年患者。提取性别,年龄,体质量,合并症[高血压、糖尿病、慢性阻塞性肺疾病(COPD)、慢性肾脏病(CKD)],入住ICU首个24 h内生命体征,实验室指标,SOFA、LODS、OASIS、SAPSⅡ、SAPS、SIRS和ECI评分及住院预后等资料。使用各评分系统绘制受试者工作特征(ROC)曲线、校准曲线,并计算净重新分类指数(NRI)、综合判别改善指数(IDI),分析与比较各评分系统对大肠埃希菌血流感染的脓毒症患者住院死亡的预测价值。结果共筛选出280例早期大肠埃希菌血培养阳性的脓毒症患者,其中住院生存组216例,死亡组64例。两组年龄、性别、BMI及SIRS评分差异均无统计学意义(P>0.05),两组生命体征、ICU住院时间、总住院时间、WBCmax、PLTmax、Lacmax、首日机械通气比例、SOFA、OASIS、LODS、SAPSⅡ、SAPS及ECI差异均有统计学意义(均P<0.05)。ROC曲线及校准曲线显示,SOFA、LODS、OASIS、SAPSⅡ、SAPS评分及ECI均能预测大肠埃希菌感染患者预后,其中SOFA和OASIS评分的预测效能最强,ROC曲线下面积(AUC)分别为0.786和0.790,截断值分别为10和39。ROC及校准曲线均显示,SIRS评分具有较差的预测能力。NRI及IDI显示,SOFA与OASIS、LODS、SAPSⅡ、SAPS等评分差异无统计学意义,但与SIRS、ECI差异有统计学意义。结论SOFA、LODS、OASIS、SAPSⅡ、SAPS及ECI评分均能预测大肠埃希菌血流感染脓毒症患者的预后,临床上可根据实际情况选择合适的评分系统及时判断患者预后。而SIRS预测能力较差,并不适用于判断大肠埃希菌血流感染脓毒症患者的预后。展开更多
基金Basic Research Foundation for Universities of the CPC Central Committee(No.2042021KF0081)Innovation Group of Natural Science Foundation of Hubei Province(2020CFA027)。
文摘Objective:To investigate the effects of Eriocitrin on the proliferation and migration of Lung adenocarcinoma(LUAD)cells A549 and H1299,and the mechanism of Epithelial-Mesenchymal Transition(EMT).Methods:The effects of different Eriocitrin on the proliferation of LUAD cells A549 and H1299 were examined by CCK8 method.EMT-associated epithelial calmodulin(E-cadherin and N-cadherin),vimentin,ferroptosis-associated protein SLC7A11,GPX4,FTH were detected by Western Blot and expression of mRNA of EMT marker molecules E-cadherin,N-cadherin,Snail were detected by qRT-PCR.Effects of saccharomyces cerevisiae suberin on ferroptosis in LUAD cells as observed by lipid reactive oxygen species(ROS)assay.Results:Eriocitrin could significantly inhibit the proliferative behavior of LUAD cells A549 and H1299 and showed a certain dose-and time-dependence.Compared with the control group,different concentrations of Eriocitrin could significantly reduce the scratch healing rate after 24 and 48 h of action,and the difference was statistically significant(P<0.01).The expression of ROS is increased,EMT-related protein E-cadherin was increased in LUAD cells A549 and H1299 compared with the control group after the intervention with Eriocitrin.N-cadherin and Vimentin expression was decreased.E-cadherin mRNA expression was increased,and N-cadherin,Snail mRNA expression was decreased,expression of ferroptosis-associated protein SLC7A11,GPX4,FTH was decreased,the difference was statistically significant(P<0.05).Conclusion:Eriocitrin may inhibit the proliferation and migration of LUAD cells by regulating the EMT pathway and has potential application in LUAD prevention and adjuvant chemotherapy.
文摘目的评价与比较序贯器官衰竭评分(SOFA)、Logistic脏器功能不全评分(LODS)、牛津急性疾病严重程度评分(OASIS)、简明急性生理功能评分Ⅱ(SAPSⅡ)、简明急性生理功能评分(SAPS)、全身炎症反应综合征(SIRS)和Elixhauser共病指数(ECI)评分系统对大肠埃希菌血流感染脓毒症患者住院病死率的预测能力。方法从MIMIC-Ⅲ(Medical Information Mart for Intensive Care)重症医学数据库中筛选出入重症监护病房(ICU)且早期血培养结果为大肠埃希菌的成年患者。提取性别,年龄,体质量,合并症[高血压、糖尿病、慢性阻塞性肺疾病(COPD)、慢性肾脏病(CKD)],入住ICU首个24 h内生命体征,实验室指标,SOFA、LODS、OASIS、SAPSⅡ、SAPS、SIRS和ECI评分及住院预后等资料。使用各评分系统绘制受试者工作特征(ROC)曲线、校准曲线,并计算净重新分类指数(NRI)、综合判别改善指数(IDI),分析与比较各评分系统对大肠埃希菌血流感染的脓毒症患者住院死亡的预测价值。结果共筛选出280例早期大肠埃希菌血培养阳性的脓毒症患者,其中住院生存组216例,死亡组64例。两组年龄、性别、BMI及SIRS评分差异均无统计学意义(P>0.05),两组生命体征、ICU住院时间、总住院时间、WBCmax、PLTmax、Lacmax、首日机械通气比例、SOFA、OASIS、LODS、SAPSⅡ、SAPS及ECI差异均有统计学意义(均P<0.05)。ROC曲线及校准曲线显示,SOFA、LODS、OASIS、SAPSⅡ、SAPS评分及ECI均能预测大肠埃希菌感染患者预后,其中SOFA和OASIS评分的预测效能最强,ROC曲线下面积(AUC)分别为0.786和0.790,截断值分别为10和39。ROC及校准曲线均显示,SIRS评分具有较差的预测能力。NRI及IDI显示,SOFA与OASIS、LODS、SAPSⅡ、SAPS等评分差异无统计学意义,但与SIRS、ECI差异有统计学意义。结论SOFA、LODS、OASIS、SAPSⅡ、SAPS及ECI评分均能预测大肠埃希菌血流感染脓毒症患者的预后,临床上可根据实际情况选择合适的评分系统及时判断患者预后。而SIRS预测能力较差,并不适用于判断大肠埃希菌血流感染脓毒症患者的预后。
