Objective To evaluate the safety and effectiveness of a vaccine based on latent membrane protein 2(LMP2)modified dendritic cells(DCs)that boosts specific responses of cytotoxic T lymphocytes(CTLs)to LMP2 before and af...Objective To evaluate the safety and effectiveness of a vaccine based on latent membrane protein 2(LMP2)modified dendritic cells(DCs)that boosts specific responses of cytotoxic T lymphocytes(CTLs)to LMP2 before and after intradermal injection in patients with nasopharyngeal carcinoma(NPC).Methods DCs were derived from peripheral blood monocytes of patients with NPC.We prepared LMP2-DCs infected by recombinant adenovirus vector expressing LMP2(rAd-LMP2).NPC patients were immunized with 2×105 LMP2-DCs by intradermal injection at week 0 and after the second and fourth weeks.Specific responses to LMP2 were detected by enzyme-linked immunospot(ELISPOT)assay at week 0 and at the fifth and eighth weeks.Local clinicians performed the follow-up and tracking of patients.Results We demonstrated that DCs derived from monocytes displayed typical DC morphologies;the expression of LMP2 in the LMP2-DCs vaccine was confirmed by immunocytochemical assay.Twenty-nine patients with NPC were enrolled in this clinical trial.The LMP2-DCs vaccine was well tolerated in all of the patients.Boosted responses to LMP2 peptide sub-pools were observed in 18 of the 29 patients with NPC.The follow-up data of 29 immunized patients from April,2010 to April 2015 indicated a five-year survival rate of 94.4%in responders and 45.5%in non-responders.Conclusion In this pilot study,we demonstrated that the LMP2-DCs vaccine is safe and effective in patients with NPC.Specific CTLs responses to LMP2 play a certain role in controlling and preventing the recurrence and metastasis of NPC,which warrants further clinical testing.展开更多
The application of air conditioning heat pump(ACHP) in electric vehicles could lead to significant electrical power saving effect. As for an air conditioning heat pump system for electric vehicles, the influence of re...The application of air conditioning heat pump(ACHP) in electric vehicles could lead to significant electrical power saving effect. As for an air conditioning heat pump system for electric vehicles, the influence of refrigerant charge amount should be investigated during the design phase. In this study, experimental method was employed to investigate the influence of the refrigerant charge amount on the performance of the ACHP system. The results showed that the refrigerant charge amount had different influence on the refrigerant properties at various locations within the system. The coefficient of performance and pressure-enthalpy diagram were calculated, and showed a close relationship with refrigerant charge amount under different compressor speeds. The degree of subcooling and the degree of superheating were recorded and the critical charge amount was determined by a typical practical test method. In addition, the critical refrigerant charge amount determined by the experimental method was also compared with two typical void fraction correlation models, and the model with consideration of two phase stream reaction of the refrigerant showed a good estimation accuracy on the critical charge amount.展开更多
基金Mega Project of Research on the Prevention and Control of HIV/AIDS,Viral Hepatitis Infectious Diseases[Grant No:2018ZX10102001]the Key Science and Technology Program of Guangxi Zhuang Autonomous Region[Grant No.14124003-3]+1 种基金the National High Technology Research and Development Program of China[Grant No.2007AA021107]and the National Basic Research Program of China[973 Program,Grant No.2011CB504800]。
文摘Objective To evaluate the safety and effectiveness of a vaccine based on latent membrane protein 2(LMP2)modified dendritic cells(DCs)that boosts specific responses of cytotoxic T lymphocytes(CTLs)to LMP2 before and after intradermal injection in patients with nasopharyngeal carcinoma(NPC).Methods DCs were derived from peripheral blood monocytes of patients with NPC.We prepared LMP2-DCs infected by recombinant adenovirus vector expressing LMP2(rAd-LMP2).NPC patients were immunized with 2×105 LMP2-DCs by intradermal injection at week 0 and after the second and fourth weeks.Specific responses to LMP2 were detected by enzyme-linked immunospot(ELISPOT)assay at week 0 and at the fifth and eighth weeks.Local clinicians performed the follow-up and tracking of patients.Results We demonstrated that DCs derived from monocytes displayed typical DC morphologies;the expression of LMP2 in the LMP2-DCs vaccine was confirmed by immunocytochemical assay.Twenty-nine patients with NPC were enrolled in this clinical trial.The LMP2-DCs vaccine was well tolerated in all of the patients.Boosted responses to LMP2 peptide sub-pools were observed in 18 of the 29 patients with NPC.The follow-up data of 29 immunized patients from April,2010 to April 2015 indicated a five-year survival rate of 94.4%in responders and 45.5%in non-responders.Conclusion In this pilot study,we demonstrated that the LMP2-DCs vaccine is safe and effective in patients with NPC.Specific CTLs responses to LMP2 play a certain role in controlling and preventing the recurrence and metastasis of NPC,which warrants further clinical testing.
基金supported by The Open Project Program of State Key Laboratory of Fire Science (No. HZ2018-KF03)Shanghai Sailing Program (No. 18YF1417900)Huaqiao University Scientific Research Foundation (No. 16BS801)
文摘The application of air conditioning heat pump(ACHP) in electric vehicles could lead to significant electrical power saving effect. As for an air conditioning heat pump system for electric vehicles, the influence of refrigerant charge amount should be investigated during the design phase. In this study, experimental method was employed to investigate the influence of the refrigerant charge amount on the performance of the ACHP system. The results showed that the refrigerant charge amount had different influence on the refrigerant properties at various locations within the system. The coefficient of performance and pressure-enthalpy diagram were calculated, and showed a close relationship with refrigerant charge amount under different compressor speeds. The degree of subcooling and the degree of superheating were recorded and the critical charge amount was determined by a typical practical test method. In addition, the critical refrigerant charge amount determined by the experimental method was also compared with two typical void fraction correlation models, and the model with consideration of two phase stream reaction of the refrigerant showed a good estimation accuracy on the critical charge amount.
