Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona fide targets in human cholangiocarcinoma cells. Methods The expression profile of microRNA-21...Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona fide targets in human cholangiocarcinoma cells. Methods The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholangiocarcinoma cell line, QBC939, was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot, respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR, locked nucleic acid in situ hybridization (LNA-ISH), and immunohistochemistry analysis. Results Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bileduct tissues (P<0.05). The dual luciferase reporter gene assay revealed endogenous microRNA-21 in cholangiocarcinoma cell line, QBC939, inhibited the luciferase reporter activities of wild-type PTEN (P<0.01) and PDCD4 (P<0.05) and had no this effect on mutated PTEN and PDCD4. Moreover, loss of microRNA-21 function led to a significant increase of PTEN and PDCD4 protein levels in QBC939 cells. Elevated microRNA-21 levels were accompanied by marked reductions of PTEN and PDCD4 expression in the same cholangiocarcinoma tissue. Conclusion microRNA-21 expression is up-regulated in human cholangiocarcinoma and PTEN, PDCD4 are direct effectors of microRNA-21.展开更多
中国正在大力发展氢能,积极实现“3060”碳排放目标,根据测算,中国将成为最大的氢能生产国和消费国之一。中国各省资源禀赋、经济发展情况各不相同,跨省际能源合作尤为重要。为研究绿色氢能跨省供应的可行性,包括氢能运输的技术可行性,...中国正在大力发展氢能,积极实现“3060”碳排放目标,根据测算,中国将成为最大的氢能生产国和消费国之一。中国各省资源禀赋、经济发展情况各不相同,跨省际能源合作尤为重要。为研究绿色氢能跨省供应的可行性,包括氢能运输的技术可行性,采用全成本(total cost of ownership,TCO)理论建立了氢能跨省运输的全成本模型。在此基础上,通过案例研究分析了江西省制氢供粤的相关情况,通过模型计算,得到了江西供粤氢能的成本为31.9元/kg,与当地氢能售价持平。同时还总结出影响外省氢能价格的主要因素,制氢电费占制氢成本的42.38%。研究结果可为中国省际绿色氢能的供给提供解决方案,也可为构建能源运输新通道提供新思路。展开更多
基金Supported by Institute of Basic Medical Sciences(2009PY13 and 2010PYZ18)the National Natural Science Foundation of China(81100608 and 30901342)
文摘Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona fide targets in human cholangiocarcinoma cells. Methods The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholangiocarcinoma cell line, QBC939, was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot, respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR, locked nucleic acid in situ hybridization (LNA-ISH), and immunohistochemistry analysis. Results Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bileduct tissues (P<0.05). The dual luciferase reporter gene assay revealed endogenous microRNA-21 in cholangiocarcinoma cell line, QBC939, inhibited the luciferase reporter activities of wild-type PTEN (P<0.01) and PDCD4 (P<0.05) and had no this effect on mutated PTEN and PDCD4. Moreover, loss of microRNA-21 function led to a significant increase of PTEN and PDCD4 protein levels in QBC939 cells. Elevated microRNA-21 levels were accompanied by marked reductions of PTEN and PDCD4 expression in the same cholangiocarcinoma tissue. Conclusion microRNA-21 expression is up-regulated in human cholangiocarcinoma and PTEN, PDCD4 are direct effectors of microRNA-21.
文摘中国正在大力发展氢能,积极实现“3060”碳排放目标,根据测算,中国将成为最大的氢能生产国和消费国之一。中国各省资源禀赋、经济发展情况各不相同,跨省际能源合作尤为重要。为研究绿色氢能跨省供应的可行性,包括氢能运输的技术可行性,采用全成本(total cost of ownership,TCO)理论建立了氢能跨省运输的全成本模型。在此基础上,通过案例研究分析了江西省制氢供粤的相关情况,通过模型计算,得到了江西供粤氢能的成本为31.9元/kg,与当地氢能售价持平。同时还总结出影响外省氢能价格的主要因素,制氢电费占制氢成本的42.38%。研究结果可为中国省际绿色氢能的供给提供解决方案,也可为构建能源运输新通道提供新思路。