Objective:To explore the therapeutic effect of Xuebijing on burn patients and its effect on pain and wound healing.Methods:The burn patients treated in our hospital were selected and divided into two groups according ...Objective:To explore the therapeutic effect of Xuebijing on burn patients and its effect on pain and wound healing.Methods:The burn patients treated in our hospital were selected and divided into two groups according to single and double numbers.The first group,the conventional treatment group(CT group):take conventional treatment to treat this group of burn patients;the second group,Xuebijing group(BWN group):take the CT group+Xuebijing combination treatment of this group of burn patients,37 cases in each group.There was no difference in general data between CT group and BWN group.To explore the differences between prothrombin time(PT),thrombin time(TT),fibrinogen(FIB),pain,secondary effects,and wound healing in CT group and BWN group The therapeutic effect of the patient and its effect on pain and wound healing.Results:Examination showed that the coagulation indexes of patients in CT group were 26.34±4.25,17.69±4.12,2.01±0.78,the coagulation indexes of patients in BWN group were 17.49±3.56,11.07±2.39,3.56±1.14,compared with CT group and BWN group In the CT group,the prothrombin time(PT)and thrombin time(TT)indicators were higher than those in the BWN group,but fibrinogen(FIB)was significantly lower than that in the BWN group(p<0.05).It can be seen from the table that before treatment,the visual analogue scoring(VAS)scores of patients in CT group and BWN group were 6.64±1.12 and 6.98±1.08,respectively,with a small difference(p>0.05).After treatment,CT group and BWN group scores They were 4.56±1.16 and 2.12±0.97 respectively.Compared with CT group and BWN group,the VAS score of CT group was significantly higher than that of BWN group(p<0.05).After treatment,the number of wound healing days in the BWN group(20.68±3.14d)was less than that in the CT group(32.89±4.25d)(P<0.05),but the wound healing rate(96.48±6.89%)was significantly higher than the CT group(81.17±5.74%)(P<0.05).In the CT group,3 patients had blood accumulation,2 infections,and 3 effusions.In the BWN group,there were 0 blood accumulations,1 infection,and 2 effusions.Compared with the CT group and the BWN group,the number and incidence of the BWN group(8.12%)Are less than CT group(21.62%)(p<0.05).The patient's leg suffered third-degree burns.After 5 weeks of treatment,the edge of the allogeneic skin on the CT group began to peel off and the epidermis fell off.Rejection.Conclusion:Conventional treatment+Xuebijing treatment,its curative effect is significant,can significantly accelerate the wound healing rate,and at the same time,reduce the incidence of secondary effects.展开更多
Objective:To investigate the effect of PGA/PLA scaffolds on tissue engineering cartilage reconstruction in knee osteoarthritis. Methods:Thirty Japanese white rabbits were divided into three groups. The first group was...Objective:To investigate the effect of PGA/PLA scaffolds on tissue engineering cartilage reconstruction in knee osteoarthritis. Methods:Thirty Japanese white rabbits were divided into three groups. The first group was healthy (group H):normal Japanese white rabbits, without knee osteoarthritis;the second group, knee osteoarthritis group ( Group k):Normal Japanese white rabbits were diagnosed with knee osteoarthritis by model preparation;Group 3, tissue engineering group (Group T):Tissue engineering cartilage reconstruction of Japanese knee white rabbits with knee osteoarthritis. 10 white rabbits per group. The cartilage histological score, HE staining, immunohistochemistry, Western blot, qRT-PCR analysis of H group, k group, T group cartilage histological score, cartilage histopathology and morphological changes, cartilage tissue The difference in Col-Ⅱ protein content and Col-Ⅱ mRNA content was used to investigate the effect of PGA/PLA scaffold material on tissue engineering cartilage reconstruction of knee osteoarthritis. Results:Cartilage tissue was scored according to international histological scoring criteria. The cartilage of the k group was severely fibrotic, the surface of the joint was irregular, and there were many fluids in the cavity, and the defect was severe. In the T group, the fibrosis phenomenon was alleviated, the surface was regular, the area of the effusion in the cavity was reduced, no depression occurred, and the surface of the joint was regular. Arthritis symptoms and cartilage tissue scores were significantly improved in group T and group k (P<0.05). The chondrocytes in the H group were densely distributed, and the k group was disorderly and sparse. In the H group, the cartilage layer of the knee joint was thick, and the cartilage layer in the k group was thin and damaged. The chondrocytes in the H group were located in the lacuna in the stroma, and there were cartilage sacs. The k group had obvious defects and no cartilage capsule structure (all P<0.05). Compared with the k group, the cartilage layer at the knee joint was thicker and the cells were densely distributed. The cartilage sac of some cells could be seen (P<0.05). There were more stromal collagen positive cells in the knee joint of group H. The number of cells in the k group was the least, the cartilage matrix was the most severely damaged, and the chondrocytes and the bone matrix were loose. Compared with group T and group k, the damage of positive cartilage matrix was reduced, and the number of cells was significantly increased (P<0.05). Result:There was a significant difference between the k group and the T group (P<0.05). Western blot was used to immunoblot the content of Col-Ⅱ protein in cartilage tissues of group H, k and T. According to the gray scale analysis, the content of Col-Ⅱ protein was the highest in group H, and the lowest in group k, group T and k. Compared with the group, the content of Col-Ⅱ protein was significantly increased (P<0.05). The expression of Col-Ⅱ mRNA in cartilage tissue of H group was the highest in group H, and the expression of Col-Ⅱ mRNA was the lowest in group k. The expression of Col-Ⅱ mRNA was significantly increased in group T and group k (all P< 0.05). Conclusions:PGA/PLA was used as scaffold material to reconstruct knee osteochondral tissue by tissue engineering method, which has obvious therapeutic effect on knee osteoarthritis.展开更多
Fuzzing is known to be one of the most effective techniques to uncover security vulnerabilities of large-scale software systems.During fuzzing,it is crucial to distribute the fuzzing resource appropriately so as to ac...Fuzzing is known to be one of the most effective techniques to uncover security vulnerabilities of large-scale software systems.During fuzzing,it is crucial to distribute the fuzzing resource appropriately so as to achieve the best fuzzing performance under a limited budget.Existing distribution strategies of American Fuzzy Lop(AFL)based greybox fuzzing focus on increasing coverage blindly without considering the metrics of code regions,thus lacking the insight regarding which region is more likely to be vulnerable and deserves more fuzzing resources.We tackle the above drawback by proposing a vulnerable region-aware greybox fuzzing approach.Specifically,we distribute more fuzzing resources towards regions that are more likely to be vulnerable based on four kinds of code metrics.We implemented the approach as an extension to AFL named RegionFuzz.Large-scale experimental evaluations validate the effectiveness and efficiency of RegionFuzz-11 new bugs including three new CVEs are successfully uncovered by RegionFuzz.展开更多
Brain size abnormality is correlated with an increased frequency of autism spectrum disorder(ASD)in offspring.Genetic analysis indicates that heterozygous mutations of the WD repeat domain 62(WDR62)are associated with...Brain size abnormality is correlated with an increased frequency of autism spectrum disorder(ASD)in offspring.Genetic analysis indicates that heterozygous mutations of the WD repeat domain 62(WDR62)are associated with ASD.However,biological evidence is still lacking.Our study showed that Wdr62 knockout(KO)led to reduced brain size with impaired learning and memory,as well as ASD-like behaviors in mice.Interestingly,Wdr62 Nex-cKO mice(depletion of WDR62 in differentiated neurons)had a largely normal brain size but with aberrant social interactions and repetitive behaviors.WDR62 regulated dendritic spinogenesis and excitatory synaptic transmission in cortical pyramidal neurons.Finally,we revealed that retinoic acid gavages significantly alleviated ASD-like behaviors in mice with WDR62 haploinsufficiency,probably by complementing the expression of ASD and synapse-related genes.Our findings provide a new perspective on the relationship between the microcephaly gene WDR62 and ASD etiology that will benefit clinical diagnosis and intervention of ASD.展开更多
We present evidence of geomagnetic storms in Mercury’s magnetosphere based on MESSENGER magnetic field observations made just before the probe impacted the planet.Our findings answer the question of whether geomagnet...We present evidence of geomagnetic storms in Mercury’s magnetosphere based on MESSENGER magnetic field observations made just before the probe impacted the planet.Our findings answer the question of whether geomagnetic storms can occur in other planetary magnetospheres.The interaction of the solar wind with Mercury’s magnetosphere is known to involve flux transfer events in the dayside magnetosphere,plasmoids and flux ropes in the magnetotail,and substorm-like processes,all of which occur morphologically similar to Earth but with significant differences.The significantly weaker magnetic field,smaller magnetosphere,and much faster timescale of processes around Mercury,when compared with Earth,enable charged particles to escape its magnetosphere more efficiently through magnetopause shadowing and direct bombard of the surface.Our analysis of MESSENGER’s data during a coronal mass ejection(CME)proves that,despite these substantial differences,a bifurcated ring current can form in Mercury’s magnetosphere that initiates magnetic storms under strong solar wind driving.展开更多
基金National natural science foundation of China(No.81201217,31300801)
文摘Objective:To explore the therapeutic effect of Xuebijing on burn patients and its effect on pain and wound healing.Methods:The burn patients treated in our hospital were selected and divided into two groups according to single and double numbers.The first group,the conventional treatment group(CT group):take conventional treatment to treat this group of burn patients;the second group,Xuebijing group(BWN group):take the CT group+Xuebijing combination treatment of this group of burn patients,37 cases in each group.There was no difference in general data between CT group and BWN group.To explore the differences between prothrombin time(PT),thrombin time(TT),fibrinogen(FIB),pain,secondary effects,and wound healing in CT group and BWN group The therapeutic effect of the patient and its effect on pain and wound healing.Results:Examination showed that the coagulation indexes of patients in CT group were 26.34±4.25,17.69±4.12,2.01±0.78,the coagulation indexes of patients in BWN group were 17.49±3.56,11.07±2.39,3.56±1.14,compared with CT group and BWN group In the CT group,the prothrombin time(PT)and thrombin time(TT)indicators were higher than those in the BWN group,but fibrinogen(FIB)was significantly lower than that in the BWN group(p<0.05).It can be seen from the table that before treatment,the visual analogue scoring(VAS)scores of patients in CT group and BWN group were 6.64±1.12 and 6.98±1.08,respectively,with a small difference(p>0.05).After treatment,CT group and BWN group scores They were 4.56±1.16 and 2.12±0.97 respectively.Compared with CT group and BWN group,the VAS score of CT group was significantly higher than that of BWN group(p<0.05).After treatment,the number of wound healing days in the BWN group(20.68±3.14d)was less than that in the CT group(32.89±4.25d)(P<0.05),but the wound healing rate(96.48±6.89%)was significantly higher than the CT group(81.17±5.74%)(P<0.05).In the CT group,3 patients had blood accumulation,2 infections,and 3 effusions.In the BWN group,there were 0 blood accumulations,1 infection,and 2 effusions.Compared with the CT group and the BWN group,the number and incidence of the BWN group(8.12%)Are less than CT group(21.62%)(p<0.05).The patient's leg suffered third-degree burns.After 5 weeks of treatment,the edge of the allogeneic skin on the CT group began to peel off and the epidermis fell off.Rejection.Conclusion:Conventional treatment+Xuebijing treatment,its curative effect is significant,can significantly accelerate the wound healing rate,and at the same time,reduce the incidence of secondary effects.
文摘Objective:To investigate the effect of PGA/PLA scaffolds on tissue engineering cartilage reconstruction in knee osteoarthritis. Methods:Thirty Japanese white rabbits were divided into three groups. The first group was healthy (group H):normal Japanese white rabbits, without knee osteoarthritis;the second group, knee osteoarthritis group ( Group k):Normal Japanese white rabbits were diagnosed with knee osteoarthritis by model preparation;Group 3, tissue engineering group (Group T):Tissue engineering cartilage reconstruction of Japanese knee white rabbits with knee osteoarthritis. 10 white rabbits per group. The cartilage histological score, HE staining, immunohistochemistry, Western blot, qRT-PCR analysis of H group, k group, T group cartilage histological score, cartilage histopathology and morphological changes, cartilage tissue The difference in Col-Ⅱ protein content and Col-Ⅱ mRNA content was used to investigate the effect of PGA/PLA scaffold material on tissue engineering cartilage reconstruction of knee osteoarthritis. Results:Cartilage tissue was scored according to international histological scoring criteria. The cartilage of the k group was severely fibrotic, the surface of the joint was irregular, and there were many fluids in the cavity, and the defect was severe. In the T group, the fibrosis phenomenon was alleviated, the surface was regular, the area of the effusion in the cavity was reduced, no depression occurred, and the surface of the joint was regular. Arthritis symptoms and cartilage tissue scores were significantly improved in group T and group k (P<0.05). The chondrocytes in the H group were densely distributed, and the k group was disorderly and sparse. In the H group, the cartilage layer of the knee joint was thick, and the cartilage layer in the k group was thin and damaged. The chondrocytes in the H group were located in the lacuna in the stroma, and there were cartilage sacs. The k group had obvious defects and no cartilage capsule structure (all P<0.05). Compared with the k group, the cartilage layer at the knee joint was thicker and the cells were densely distributed. The cartilage sac of some cells could be seen (P<0.05). There were more stromal collagen positive cells in the knee joint of group H. The number of cells in the k group was the least, the cartilage matrix was the most severely damaged, and the chondrocytes and the bone matrix were loose. Compared with group T and group k, the damage of positive cartilage matrix was reduced, and the number of cells was significantly increased (P<0.05). Result:There was a significant difference between the k group and the T group (P<0.05). Western blot was used to immunoblot the content of Col-Ⅱ protein in cartilage tissues of group H, k and T. According to the gray scale analysis, the content of Col-Ⅱ protein was the highest in group H, and the lowest in group k, group T and k. Compared with the group, the content of Col-Ⅱ protein was significantly increased (P<0.05). The expression of Col-Ⅱ mRNA in cartilage tissue of H group was the highest in group H, and the expression of Col-Ⅱ mRNA was the lowest in group k. The expression of Col-Ⅱ mRNA was significantly increased in group T and group k (all P< 0.05). Conclusions:PGA/PLA was used as scaffold material to reconstruct knee osteochondral tissue by tissue engineering method, which has obvious therapeutic effect on knee osteoarthritis.
基金(partially)supported by the National Key Research and Development Program of China under Grant No.2017YFA0700604the National Natural Science Foundation of China under Grant Nos.62032010 and 61802168+1 种基金the Leading-Edge Technology Program of Jiangsu Natural Science Foundation under Grant No.BK20202001the 2021 Double Entrepreneurship Big Data and Theoretical Research Project of Nanjing University.
文摘Fuzzing is known to be one of the most effective techniques to uncover security vulnerabilities of large-scale software systems.During fuzzing,it is crucial to distribute the fuzzing resource appropriately so as to achieve the best fuzzing performance under a limited budget.Existing distribution strategies of American Fuzzy Lop(AFL)based greybox fuzzing focus on increasing coverage blindly without considering the metrics of code regions,thus lacking the insight regarding which region is more likely to be vulnerable and deserves more fuzzing resources.We tackle the above drawback by proposing a vulnerable region-aware greybox fuzzing approach.Specifically,we distribute more fuzzing resources towards regions that are more likely to be vulnerable based on four kinds of code metrics.We implemented the approach as an extension to AFL named RegionFuzz.Large-scale experimental evaluations validate the effectiveness and efficiency of RegionFuzz-11 new bugs including three new CVEs are successfully uncovered by RegionFuzz.
基金This work was supported by the National Natural Science Foundation of China(31970920,31921002,and 31430037).
文摘Brain size abnormality is correlated with an increased frequency of autism spectrum disorder(ASD)in offspring.Genetic analysis indicates that heterozygous mutations of the WD repeat domain 62(WDR62)are associated with ASD.However,biological evidence is still lacking.Our study showed that Wdr62 knockout(KO)led to reduced brain size with impaired learning and memory,as well as ASD-like behaviors in mice.Interestingly,Wdr62 Nex-cKO mice(depletion of WDR62 in differentiated neurons)had a largely normal brain size but with aberrant social interactions and repetitive behaviors.WDR62 regulated dendritic spinogenesis and excitatory synaptic transmission in cortical pyramidal neurons.Finally,we revealed that retinoic acid gavages significantly alleviated ASD-like behaviors in mice with WDR62 haploinsufficiency,probably by complementing the expression of ASD and synapse-related genes.Our findings provide a new perspective on the relationship between the microcephaly gene WDR62 and ASD etiology that will benefit clinical diagnosis and intervention of ASD.
基金supported by Major Project of Chinese National Programs for Fundamental Research and Development(Grant No.2021YFA0718600)China Space Agency Project(Grant No.D020301)+2 种基金the National Natural Science Foundation of China(Grant No.42011530080)financial support from the Canadian Space Agency and NSERCpartially supported by National Science Foundation(Grant No.AGS-1352669)。
文摘We present evidence of geomagnetic storms in Mercury’s magnetosphere based on MESSENGER magnetic field observations made just before the probe impacted the planet.Our findings answer the question of whether geomagnetic storms can occur in other planetary magnetospheres.The interaction of the solar wind with Mercury’s magnetosphere is known to involve flux transfer events in the dayside magnetosphere,plasmoids and flux ropes in the magnetotail,and substorm-like processes,all of which occur morphologically similar to Earth but with significant differences.The significantly weaker magnetic field,smaller magnetosphere,and much faster timescale of processes around Mercury,when compared with Earth,enable charged particles to escape its magnetosphere more efficiently through magnetopause shadowing and direct bombard of the surface.Our analysis of MESSENGER’s data during a coronal mass ejection(CME)proves that,despite these substantial differences,a bifurcated ring current can form in Mercury’s magnetosphere that initiates magnetic storms under strong solar wind driving.
