A new hybrid organic-inorganic monolithic cartridge was synthesized and used as the selective sorbent for the extraction and purification of quercetin and myricetin from Chamaecyparis obtusa via a solid-phase extracti...A new hybrid organic-inorganic monolithic cartridge was synthesized and used as the selective sorbent for the extraction and purification of quercetin and myricetin from Chamaecyparis obtusa via a solid-phase extraction method.The morphology of the monolithic material was examined by field emission-scanning electron microscopy and a Brunauer-Emmett-Teller（BET） test.The adsorption capacity of the obtained material for quercetin and myricetin was investigated by fitting the adsorption data to four different adsorption equations,of which the LangmuirFreundlich isotherm was selected as the most suitable model.Under optimized conditions,good calibration curves were observed at nine concentrations ranged from 0.5 μg/mL to 100.0 μg/mL of quercetin and myricetin.The extraction recovery ranged from 74.5％ to 84.6％ and the inter-and intra-day relative standard deviations were ＜6％.This type of hybrid monolith has potential for the separation and purification of bioactive compounds from natural plant extracts.展开更多
文摘A new hybrid organic-inorganic monolithic cartridge was synthesized and used as the selective sorbent for the extraction and purification of quercetin and myricetin from Chamaecyparis obtusa via a solid-phase extraction method.The morphology of the monolithic material was examined by field emission-scanning electron microscopy and a Brunauer-Emmett-Teller（BET） test.The adsorption capacity of the obtained material for quercetin and myricetin was investigated by fitting the adsorption data to four different adsorption equations,of which the LangmuirFreundlich isotherm was selected as the most suitable model.Under optimized conditions,good calibration curves were observed at nine concentrations ranged from 0.5 μg/mL to 100.0 μg/mL of quercetin and myricetin.The extraction recovery ranged from 74.5％ to 84.6％ and the inter-and intra-day relative standard deviations were ＜6％.This type of hybrid monolith has potential for the separation and purification of bioactive compounds from natural plant extracts.
