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茶小绿叶蝉粘样蛋白EfMLP基因的克隆、鉴定及功能分析
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作者 何雪怡 雷雨欢 +7 位作者 宋仕月 夏露霞 王世宇 马成文 魏可欣 王梦馨 潘铖 韩宝瑜 《昆虫学报》 CAS CSCD 北大核心 2024年第2期151-162,共12页
【目的】探明茶小绿叶蝉Empoasca flavescens粘样蛋白EfMLP基因的分子特征、表达模式和生物学功能。【方法】基于茶小绿叶蝉转录组数据,PCR克隆获得4条EfMLP基因的全长cDNA序列并进行生物信息学分析;qRT-PCR检测EfMLP基因在茶小绿叶蝉... 【目的】探明茶小绿叶蝉Empoasca flavescens粘样蛋白EfMLP基因的分子特征、表达模式和生物学功能。【方法】基于茶小绿叶蝉转录组数据,PCR克隆获得4条EfMLP基因的全长cDNA序列并进行生物信息学分析;qRT-PCR检测EfMLP基因在茶小绿叶蝉不同发育阶段(卵、1-5龄若虫和初羽化雌雄成虫)及初羽化成虫不同组织(体壁、脂肪体、唾液腺、肠道、卵巢和精巢)中的表达量;通过饲喂法利用RNAi沉默茶小绿叶蝉5龄若虫EfMLP2和EfMLP4,生物测定分析EfMLP基因沉默后茶小绿叶蝉的存活率。【结果】获得4条茶小绿叶蝉EfMLP基因全长cDNA序列,分别命名为EfMLP1(GenBank登录号:OR504428),EfMLP2(GenBank登录号:OR504429),EfMLP3(GenBank登录号:OR504430)和EfMLP4(GenBank登录号:OR504431),这4条EfMLP基因编码蛋白均具有O-联糖基化位点形成粘蛋白结构域(mucin domain, MD)且该结构域包含高度重复的串联重复序列,其中EfMLP3和EfMLP4的氨基酸序列含有保守的2型几丁质结合域(chitin binding domain, CBD)。系统发育分析结果表明,EfMLP被分到两个不同的分支上,属于两种不同的MLP类型,该结果与昆虫的分类地位无相关性,猜测可能与其功能分化相关。EfMLP1和EfMLP2在茶小绿叶蝉初羽化雌雄成虫和初羽化成虫唾液腺中均特异性高表达,而EfMLP3和EfMLP4的表达在卵、若虫和成虫等不同发育阶段和初羽化成虫脂肪体等多个组织中均可被检测到。与饲喂dsGFP对照组比较,饲喂dsEfMLP2和dsEfMLP4可分别有效抑制茶小绿叶蝉体内EfMLP2和EfMLP4的表达量,并可显著降低茶小绿叶蝉的存活率。【结论】EfMLP在茶小绿叶蝉取食中扮演重要的角色,可作为开发基于RNAi的叶蝉防治技术的潜在靶标。 展开更多
关键词 茶小绿叶蝉 粘样蛋白 基因克隆 表达模式 RNA干扰
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Invariant measures for the strong-facilitated exclusion process
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作者 lei yu-huan SU Zhong-gen 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2023年第3期317-337,共21页
Consider a generalized model of the facilitated exclusion process, which is a onedimensional exclusion process with a dynamical constraint that prevents the particle at site x from jumping to x+1 (or x-1) if the sites... Consider a generalized model of the facilitated exclusion process, which is a onedimensional exclusion process with a dynamical constraint that prevents the particle at site x from jumping to x+1 (or x-1) if the sites x-1, x-2 (or x+1, x+2) are empty. It is nongradient and lacks invariant measures of product form. The purpose of this paper is to identify the invariant measures and to show that they satisfy both exponential decay of correlations and equivalence of ensembles. These properties will play a pivotal role in deriving the hydrodynamic limit. 展开更多
关键词 equivalence of ensembles exclusion process facilitated dynamic invariant measure
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Tracy-Widom distribution, Airy2 process and its sample path properties
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作者 SU Zhong-gen lei yu-huan SHEN Tian 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2021年第1期128-158,共31页
Tracy-Widom distribution was rst discovered in the study of largest eigenvalues of high dimensional Gaussian unitary ensembles(GUE),and since then it has appeared in a number of apparently distinct research elds.It is... Tracy-Widom distribution was rst discovered in the study of largest eigenvalues of high dimensional Gaussian unitary ensembles(GUE),and since then it has appeared in a number of apparently distinct research elds.It is believed that Tracy-Widom distribution have a universal feature like classic normal distribution.Airy2 process is de ned through nite dimensional distributions with Tracy-Widom distribution as its marginal distributions.In this introductory survey,we will briey review some basic notions,intuitive background and fundamental properties concerning Tracy-Widom distribution and Airy2 process.For sake of reading,the paper starts with some simple and well-known facts about normal distributions,Gaussian processes and their sample path properties. 展开更多
关键词 Airy2 process Dyson Brownian motion Tracy-Widom distribution sample path property.
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