DNA vaccines encoding a viral protein have been shown to induce antiviral immune responses and provide pro-tection against subsequent viral challenge. The present article deals with the efficacy of a DNA vaccine great...DNA vaccines encoding a viral protein have been shown to induce antiviral immune responses and provide pro-tection against subsequent viral challenge. The present article deals with the efficacy of a DNA vaccine greatly im-proved by the simultaneous expression of HBsAg and interferon-T gene. We constructed a dual expression vector pHIN encoding the HBsAg of Hepatitis B virus and murine IFN-T which are connected with Internal Ribosome Entry Site（IRES）. Mice immunized with this dual expression DNA vaccine exhibited the enhancement of cellular immune response and increased the production of anti-HBV surface antibody, compared with the mice of single gene expres-sion control. Taken together, these results demonstrate that the application of a cytokine gene in a DNA vaccine for-mulation as an adjuvant can improve its immunigenicity.展开更多
文摘DNA vaccines encoding a viral protein have been shown to induce antiviral immune responses and provide pro-tection against subsequent viral challenge. The present article deals with the efficacy of a DNA vaccine greatly im-proved by the simultaneous expression of HBsAg and interferon-T gene. We constructed a dual expression vector pHIN encoding the HBsAg of Hepatitis B virus and murine IFN-T which are connected with Internal Ribosome Entry Site（IRES）. Mice immunized with this dual expression DNA vaccine exhibited the enhancement of cellular immune response and increased the production of anti-HBV surface antibody, compared with the mice of single gene expres-sion control. Taken together, these results demonstrate that the application of a cytokine gene in a DNA vaccine for-mulation as an adjuvant can improve its immunigenicity.
