Screening and characterization of a novel ruminal cellulase gene(Umcel-1) from a metagenomic library of gayal(Bos frontalis)

Gayal is a rare semi-wild bovine species found in the Indo-China. They can graze grasses, including bamboo leaves, as well as reeds and other plant species, and grow to higher mature live weights than Yunnan Yellow cattle maintained in similar harsh environments. The aim of this study was to identify specific cellulase in the gayal rumen. A metagenomic fosmid library was constructed using genomic DNA isolated from the ruminal contents of four adult gayals. This library contained 38400 clones with an average insert size of 35.5 kb. The Umcel-1 gene was isolated from this library. Investigation of the cellulase activity of 24 random clones led to the identification of the Umcel-1 gene, which exhibited the most potent cellulase activity. Sequencing the Umcel-1 gene revealed that it contained an open reading frame of 942 base pairs that encoded a product of 313 amino acids. The putative gene Umcel-1 product belonged to the glycosyl hydrolase family 5 and showed the highest homology to the cellulase （GenBank accession no. YP_004310852.1 ） from Clostridium lentocellum DSM 5427, with 44% identity and 62% similarity. The Umcel-1 gene was heterologously expressed in Escherichia coil BL21, and recombinant Umcel-1 was purified. The activity of purified recombinant Umcel-1 was assessed, and the results revealed that it hydrolyzed carboxymethyl cellulose with optimal activity at pH 5.5 and 45~C. To our knowledge, this study provides the first evidence for a cellulase produced by bacteria in gayal rumen.

经椎间孔镜联合通痹活络汤治疗腰椎间盘突出症疗效观察

目的:观察腰椎间盘突出症采用经椎间孔镜联合通痹活络汤的治疗效果。方法:选择腰椎间盘突出症患者80例,随机分为两组各40例,对照组采用经椎间孔镜治疗,治疗组在对照组基础上加用通痹活络汤治疗。结果:治疗组VAS、JOA、ODI评分改善优秀于对照组,总有效率高于对照组,均有显著性差异(P<0.05)。结论:腰椎间盘突出症采用经椎间孔镜联合通痹活络汤治疗较单纯采用经椎间孔镜治疗疗效更好。

院校文职教员教学能力培树提升对策研究

全面贯彻新时代军事教育方针,实施人才强军战略和深化院校改革创新,根本在培养人才。文职教员作为院校教育体系的重要组成部分,其教学能力直接影响院校人才培养质量。随着军队改革的持续推进,院校文职教员队伍逐渐庞大,从思想观念、培育机制、传帮带作用、激励机制四个层面剖析当前其教学能力培树提升所面临的突出问题,提出锻造向战为战的高素质文职教员队伍、打造文职教员教学能力提升的教培模式、建立健全文职教员的传帮带机制、内驱院校文职教员的拴心留人工作等相关对策。

中医中药联合快速康复外科在老年髋部骨折围手术期的应用

目的:探讨中医中药联合快速康复外科(Chinese medicine in enhanced recovery aftersurgery,ERAS)在老年髋部骨折患者围手术期的应用及效果。方法:选取清远市中医院关节外科2016~2018年100例75岁以上髋部骨折,运用随机数字表法分为中医中药联合ERAS组(优化组)和常规组,优化组围手术期予以中医中药+快速康复理念;常规组予以关节外科病房一般常规处理等,观察记录两组患者围手术期的术后疼痛评分(visual analogue score,VAS)、术后不良反应。结果:优化组患者术后VAS评分降低,术后谵妄、恶心呕吐、腹胀的发生率降低,下肢深静脉血栓(DVT)发生率低,差异均有统计学意义(P<0.05)。结论:中医中药联合ERAS,可有效的减轻老年髋部骨折围手术期应激水平,减少术后不良反应,使患者术后获得快速康复,值得在临床中推广应用。

椎间孔镜技术联合中医药综合治疗腰椎间盘突出症的临床研究

目的:探讨椎间孔镜技术联合中药、电针等中医综合疗法治疗腰椎间盘突出症的疗效。方法:将80例腰椎间盘突出症患者随机分为观察组和对照组各40例,对照组给予椎间孔镜手术和常规功能锻炼,观察组则联合中药、电针等疗法,比较两组临床疗效。结果:观察组总有效率为97.5%,显著高于对照组的82.5%(P<0.05);观察组VAS、JOA、ODI评分改善程度均显著优于对照组(P<0.05)。结论:腰椎间盘突出症在椎间孔镜术后,联合中药、电针等中医综合治疗方案,可显著提高临床疗效,降低疼痛程度,改善腰椎功能,提高生存质量。