Potato (Solanum tuberosum L.) is the third most important food crop worldwide after wheat and rice in terms of human consumption. A critical domestication trait for potato was the decrease of toxic steroidal glycoalka...Potato (Solanum tuberosum L.) is the third most important food crop worldwide after wheat and rice in terms of human consumption. A critical domestication trait for potato was the decrease of toxic steroidal glycoalkaloids (SGAs) in tuber flesh. Here, we used a diploid F2 segregating population derived from a cross between S. tuberosum and the wild potato species Solanum chacoense to map the quantitative trait loci (QTLs) associated with the regulation of SGAs content in tuber flesh. In a three-year study, we identified two QTLs on chromosomes 2 and 8 affecting SGAs content in tuber flesh. The QTL on chromosome 8 harbors 38 genes that are co-expressed with the GLYCOALKALOID METABOLISM genes. These findings lay the foundation for exploiting the genes controlling SGAs content in tuber flesh and they provide a theoretical basis for the use of wild germplasm in potato breeding.展开更多
文摘目的miRNA可作为预测糖尿病发生和发展的生物标志物,探索miR-1181在2型糖尿病(Type 2Diabetes Mellitus,T2DM)患者的表达情况。方法运用Agilent miRNA芯片对T2DM患者(n=5)和正常对照组(n=5)血浆样本进行差异筛选,荧光定量PCR技术(quantitative Real Time PCR,qRT-PCR)验证出差异表达的miRNA;通过生物信息学方法预测其靶基因并绘制miRNA-靶基因-代谢通路关系网络图,扩大样本检测血浆中差异表达的miRNA:miR-1181和靶基因MAP2K2、MAPK12表达水平。结果miRNA芯片筛选和qRT-PCR验证出与T2DM相关的差异表达miRNA:miR-1181,生物信息学分析得出miR-1181靶基因有CCND1、PI3KR2、MAP2K2和MAPK12;T2DM患者血浆中miR-1181的表达水平明显下降(P<0.001),而靶基因MAPK12 mRNA的表达水平明显升高(P<0.01)。结论2型糖尿病患者血浆miR-1181水平降低,可能与其抑制靶基因MAPK12对MAPK通路影响有关。
基金supported by the Agricultural Science and Technology Innovation Program from the Chinese Academy of Agricultural Sciences(ASTIP-CAAS)the Agricultural Science and Technology Innovation Program Cooperation and Innovation Mission,CAAS(CAAS-XTCX2016)+3 种基金the Advanced Technology Talents in Yunnan Province,China 2013HA025)the PhD Scholar Newcomer Award in Yunnan Province,China(01701205020516025)the Graduate Research and Innovation Fund Project,China(yjs201679)supported by the Ministry of Agriculture and Rural Affairs of China,the Shenzhen Municipal and Dapeng District Governments,China
文摘Potato (Solanum tuberosum L.) is the third most important food crop worldwide after wheat and rice in terms of human consumption. A critical domestication trait for potato was the decrease of toxic steroidal glycoalkaloids (SGAs) in tuber flesh. Here, we used a diploid F2 segregating population derived from a cross between S. tuberosum and the wild potato species Solanum chacoense to map the quantitative trait loci (QTLs) associated with the regulation of SGAs content in tuber flesh. In a three-year study, we identified two QTLs on chromosomes 2 and 8 affecting SGAs content in tuber flesh. The QTL on chromosome 8 harbors 38 genes that are co-expressed with the GLYCOALKALOID METABOLISM genes. These findings lay the foundation for exploiting the genes controlling SGAs content in tuber flesh and they provide a theoretical basis for the use of wild germplasm in potato breeding.
