Objective:To investigate the possible mechanism of microRNA-9-5p(miR-9-5p)and Ras homologous gene family A(RHOA)in aluminum-induced cognitive dysfunction in rats.Methods:According to the principle of randomization,48 ...Objective:To investigate the possible mechanism of microRNA-9-5p(miR-9-5p)and Ras homologous gene family A(RHOA)in aluminum-induced cognitive dysfunction in rats.Methods:According to the principle of randomization,48 Wistar rats were randomly divided into four groups(n=12)of blank control,low dose,medium dose and high dose.The blank control group was gavaged daily saline,and the other three dose groups were given daily gavage AlCl3 aqueous solution at three doses of 25 mg/kg,50 mg/kg,and 100 mg/kg to create a rat model of cognitive impairment for three months.The water maze(MWM)positioning navigation experiment was used to record the time t(s),namely,the incubation period,on the platform of rats,and the incubation period of each group was used to determine whether the rats in the infected group had learning and memory impairment.Hematoxylin-eosin(HE)and Nissl stains observed the pathological changes of nerve cells in the hippocampus of the four groups.Western blot detected the protein expression levels of RHOA and cranial neurotrophic factor(BDNF)in fresh rat hippocampal tissues.RT-qPCR detected the mRNA expression of miR-9-5p,RHOA,and BDNF in rat hippocampal tissues.Results:The results of Morris water maze positioning navigation test showed that the incubation period of each group was calculated on the 1st,3rd and 5th days of the experiment,and the motor incubation period of the infected group was higher than that of the control group.The results of HE staining showed that the rat nerve cells in the control group were morphologically intact,the staining was clear,the nucleus was clearly visible,and the edge of the cell membrane was sharp.The rat neurons in the infected group were damaged to varying degrees,the nucleus gradually dissolved,the cytoplasmic staining became deeper,the edges of the cell membrane were blurred and disordered,and the cells were deformed and arranged disordered.The results of Nissl staining showed that the well-stained Nissl body particles were visible in the nerve cells of rats in the control group,and the dissipation of Nissl bodies in the nerve cells of the infected group was reduced,and the staining was shallow.The results of RT-qPCR showed that compared with the control group,the mRNA expression of miR-9-5p and BDNF was decreased in the infected group,and the mRNA expression of RHOA was increased(P<0.05 or P<0.001).The Western blot results showed that compared with the control group,the relative expression of BDNF in the three infected groups was decreased,and the relative expression of RHOA increased(P<0.05).Conclusion:In aluminum-induced cognitive impairment,miR-9-5p is downregulated and RHOA is upregulatd.展开更多
针对沥青洒布车横向洒布不均匀现象,对喷嘴的结构参数进行优化,以改善沥青洒布过程中的雾化质量。基于正交试验原理设计以喷嘴半球直径、切槽深度、切槽角为优化参数,以索特尔平均直径D_(32)及分布均匀性指数N为雾化特性指标的仿真方案...针对沥青洒布车横向洒布不均匀现象,对喷嘴的结构参数进行优化,以改善沥青洒布过程中的雾化质量。基于正交试验原理设计以喷嘴半球直径、切槽深度、切槽角为优化参数,以索特尔平均直径D_(32)及分布均匀性指数N为雾化特性指标的仿真方案。将VOF(Volume of Fluid)方法与DPM(Discrete Phase Model)方法进行耦合,通过VOF-to-DPM方法对喷嘴的试验方案进行仿真分析,并结合响应面法建立回归模型,优化求解获得最佳参数组合。结果表明:喷嘴的半球直径、切槽深度、切槽角对雾化特性有显著影响,且半球直径与切槽角之间存在显著的交互作用;喷嘴优化后在雾化质量上有显著提升,D_(32)减小了25.26%,N增加了10.27%。展开更多
基金National Natural Science Foundation Project of China(No.31560294)Guangxi Degree and Postgraduate Education Reform Project in 2021(No.JGY2021208)。
文摘Objective:To investigate the possible mechanism of microRNA-9-5p(miR-9-5p)and Ras homologous gene family A(RHOA)in aluminum-induced cognitive dysfunction in rats.Methods:According to the principle of randomization,48 Wistar rats were randomly divided into four groups(n=12)of blank control,low dose,medium dose and high dose.The blank control group was gavaged daily saline,and the other three dose groups were given daily gavage AlCl3 aqueous solution at three doses of 25 mg/kg,50 mg/kg,and 100 mg/kg to create a rat model of cognitive impairment for three months.The water maze(MWM)positioning navigation experiment was used to record the time t(s),namely,the incubation period,on the platform of rats,and the incubation period of each group was used to determine whether the rats in the infected group had learning and memory impairment.Hematoxylin-eosin(HE)and Nissl stains observed the pathological changes of nerve cells in the hippocampus of the four groups.Western blot detected the protein expression levels of RHOA and cranial neurotrophic factor(BDNF)in fresh rat hippocampal tissues.RT-qPCR detected the mRNA expression of miR-9-5p,RHOA,and BDNF in rat hippocampal tissues.Results:The results of Morris water maze positioning navigation test showed that the incubation period of each group was calculated on the 1st,3rd and 5th days of the experiment,and the motor incubation period of the infected group was higher than that of the control group.The results of HE staining showed that the rat nerve cells in the control group were morphologically intact,the staining was clear,the nucleus was clearly visible,and the edge of the cell membrane was sharp.The rat neurons in the infected group were damaged to varying degrees,the nucleus gradually dissolved,the cytoplasmic staining became deeper,the edges of the cell membrane were blurred and disordered,and the cells were deformed and arranged disordered.The results of Nissl staining showed that the well-stained Nissl body particles were visible in the nerve cells of rats in the control group,and the dissipation of Nissl bodies in the nerve cells of the infected group was reduced,and the staining was shallow.The results of RT-qPCR showed that compared with the control group,the mRNA expression of miR-9-5p and BDNF was decreased in the infected group,and the mRNA expression of RHOA was increased(P<0.05 or P<0.001).The Western blot results showed that compared with the control group,the relative expression of BDNF in the three infected groups was decreased,and the relative expression of RHOA increased(P<0.05).Conclusion:In aluminum-induced cognitive impairment,miR-9-5p is downregulated and RHOA is upregulatd.
文摘针对沥青洒布车横向洒布不均匀现象,对喷嘴的结构参数进行优化,以改善沥青洒布过程中的雾化质量。基于正交试验原理设计以喷嘴半球直径、切槽深度、切槽角为优化参数,以索特尔平均直径D_(32)及分布均匀性指数N为雾化特性指标的仿真方案。将VOF(Volume of Fluid)方法与DPM(Discrete Phase Model)方法进行耦合,通过VOF-to-DPM方法对喷嘴的试验方案进行仿真分析,并结合响应面法建立回归模型,优化求解获得最佳参数组合。结果表明:喷嘴的半球直径、切槽深度、切槽角对雾化特性有显著影响,且半球直径与切槽角之间存在显著的交互作用;喷嘴优化后在雾化质量上有显著提升,D_(32)减小了25.26%,N增加了10.27%。
