Objective This literature review aims to summarize the methods of isolation, expansion, preservation of human umbilical cord mesenchymal stem cells （hUCMSCs）, for comprehensive practical use in preclinical research ...Objective This literature review aims to summarize the methods of isolation, expansion, preservation of human umbilical cord mesenchymal stem cells （hUCMSCs）, for comprehensive practical use in preclinical research and clinical trials. differentiation and understanding and Data sources All the literature reviewed was published over the last 10 years and is listed in PubMed and Chinese National Knowledge Infrastructure （CNKI）. Studies were retrieved using the key word ＂human umbilical cord mesenchymal stem cells＂. Results Explants culture and enzymatic digestion are two methods to isolate hUCMSCs from WJ and there are modifications to improve these methods. Culture conditions may affect the expansion and differentiating orientations of hUCMSCs. In addition, hUCMSCs can maintain their multi-potential effects after being properly frozen and thawed. Conclusion Considering their multi-potential, convenient and non-invasive accessibility, low immunogenicity and the reported therapeutic effects in several different preclinical animal models, hUCMSCs have immense scope in regeneration medicine as a substitute for MSCs derived from bone marrow or umbilical cord blood.展开更多
文摘Objective This literature review aims to summarize the methods of isolation, expansion, preservation of human umbilical cord mesenchymal stem cells （hUCMSCs）, for comprehensive practical use in preclinical research and clinical trials. differentiation and understanding and Data sources All the literature reviewed was published over the last 10 years and is listed in PubMed and Chinese National Knowledge Infrastructure （CNKI）. Studies were retrieved using the key word ＂human umbilical cord mesenchymal stem cells＂. Results Explants culture and enzymatic digestion are two methods to isolate hUCMSCs from WJ and there are modifications to improve these methods. Culture conditions may affect the expansion and differentiating orientations of hUCMSCs. In addition, hUCMSCs can maintain their multi-potential effects after being properly frozen and thawed. Conclusion Considering their multi-potential, convenient and non-invasive accessibility, low immunogenicity and the reported therapeutic effects in several different preclinical animal models, hUCMSCs have immense scope in regeneration medicine as a substitute for MSCs derived from bone marrow or umbilical cord blood.
