Establishment of NaLuF_(4):15%Tb-based low dose X-PDT agent and its application on efficient antitumor therapy

X-ray excited photodynamic therapy(X-PDT)is the bravo answer of photodynamic therapy(PDT)for deep-seated tumors,as it employs X-ray as the irradiation source to overcome the limitation of light penetration depth.However,high X-ray irradiation dose caused organ lesions and side effects became the major barrier to X-PDT application.To address this issue,this work employed a classic-al co-precipitation reaction to synthesize NaLuF_(4):15%Tb^(3+)(NLF)with an average particle size of(23.48±0.91)nm,which was then coupled with the photosensitizer merocyanine 540(MC540)to form the X-PDT system NLF-MC540 with high production of singlet oxygen.The system could induce antitumor efficacy to about 24%in relative low dose X-ray irradiation range(0.1-0.3 Gy).In vivo,when NLF-MC540 irradiated by 0.1 Gy X-ray,the tumor inhibition percentage reached 89.5%±5.7%.The therapeutic mechanism of low dose X-PDT was found.A significant increase of neutrophils in serum was found on the third day after X-PDT.By immunohistochemical staining of tumor sections,the Ly6G^(+),CD8^(+),and CD11c^(+)cells infiltrated in the tumor microenvironment were studied.Utilizing the bilat-eral tumor model,the NLF-MC540 with 0.1 Gy X-ray irradiation could inhibit both the primary tumor and the distant tumor growth.De-tected by enzyme linked immunosorbent assay(ELISA),two cytokines IFN-γand TNF-αin serum were upregulated 7 and 6 times than negative control,respectively.Detected by enzyme linked immune spot assay(ELISPOT),the number of immune cells attributable to the IFN-γand TNF-αlevels in the group of low dose X-PDT were 14 and 6 times greater than that in the negative control group,respectively.Thus,it conclude that low dose X-PDT system could successfully upregulate the levels of immune cells,stimulate the secretion of cy-tokines(especially IFN-γand TNF-α),activate antitumor immunity,and finally inhibit colon tumor growth.

Development of a High-throughput Sequencing Platform for Detection of Viral Encephalitis Pathogens Based on Amplicon Sequencing

Objective Viral encephalitis is an infectious disease severely affecting human health.It is caused by a wide variety of viral pathogens,including herpes viruses,flaviviruses,enteroviruses,and other viruses.The laboratory diagnosis of viral encephalitis is a worldwide challenge.Recently,high-throughput sequencing technology has provided new tools for diagnosing central nervous system infections.Thus,In this study,we established a multipathogen detection platform for viral encephalitis based on amplicon sequencing.Methods We designed nine pairs of specific polymerase chain reaction(PCR)primers for the 12 viruses by reviewing the relevant literature.The detection ability of the primers was verified by software simulation and the detection of known positive samples.Amplicon sequencing was used to validate the samples,and consistency was compared with Sanger sequencing.Results The results showed that the target sequences of various pathogens were obtained at a coverage depth level greater than 20×,and the sequence lengths were consistent with the sizes of the predicted amplicons.The sequences were verified using the National Center for Biotechnology Information BLAST,and all results were consistent with the results of Sanger sequencing.Conclusion Amplicon-based high-throughput sequencing technology is feasible as a supplementary method for the pathogenic detection of viral encephalitis.It is also a useful tool for the high-volume screening of clinical samples.

2023 Guideline for the management of hypertension in the elderly population in China

Preamble Hypertension is one of the most common chronic diseases and the primary risk factor for stroke,myocardial infarction and even cardiovascular death.More than half of the elderly population has high blood pressure.Elderly people are a unique group,and strategies for the prevention,diagnosis,evaluation,and treatment of hypertension in elderly individuals differ significantly from those in the general population.

Subclinical impairment of left ventricular myocardium function in type 2 diabetes mellitus patients with or without hypertension

BACKGROUND Cardiovascular disease has been the leading cause of morbidity and mortality for type 2 diabetes mellitus(T2DM)patients over the last decade.AIM To determine whether layer-specific global longitudinal strain(GLS)combined with peak strain dispersion(PSD)can be used to assess left ventricle(LV)myocardium systolic dysfunction in T2DM patients or without hypertension(HP).METHODS We enrolled 97 T2DM patients,70 T2DM+HP patients and 101 healthy subjects.Layer-specific GLS and PSD were calculated by EchoPAC software in apical three-,four-and two-chamber views.GLS of the epimyocardial,middle-layer and endomyocardial(GLSepi,GLSmid,and GLSendo)were measured and recorded.Receiver operating characteristic analysis was performed to detect LV myocardium systolic dysfunction in T2DM patients.RESULTS There were significant differences in GLSepi,GLSmid,GLSendo,and PSD between healthy subjects,T2DM patients and T2DM patients with HP(P<0.001).Trend tests yielded the ranking of healthy subjects>T2DM patients>T2DM with HP patients in the absolute values of GLSepi,GLSmid and GLSendo(P<0.001),while PSD was ranked healthy subjects<T2DM<T2DM with HP(P<0.001).Layer-specific GLS and PSD had high diagnostic efficiency for detecting LV myocardium systolic dysfunction in T2DM patients,however,the area under the curve(AUC)for layer-specific GLS and PSD combined was significantly higher than the AUCs for the individual indices(P<0.05).CONCLUSION Layer-specific GLS and PSD were associated with LV myocardium systolic dysfunction in T2DM patients,T2DM patients with HP.T2DM patients with HP have more severe LV myocardium systolic dysfunction than T2DM patients without HP and normal control patients.The combination of layer-specific GLS and PSD may provide additional prognostic information for T2DM patients with or without HP.

Evaluation of left ventricular systolic function in type 2 diabetes mellitus patients with and without peripheral vascular disease

BACKGROUND Peripheral vascular disease(PVD)is a common complication of type 2 diabetes mellitus(T2DM).Patients with T2DM have twice the risk of PVD as nondiabetic patients.AIM To evaluate left ventricular(LV)systolic function by layer-specific global longitudinal strain(GLS)and peak strain dispersion(PSD)in T2DM patients with and without PVD.METHODS Sixty-five T2DM patients without PVD,57 T2DM patients with PVD and 63 normal controls were enrolled in the study.Layer-specific GLS[GLS of the epimyocardium(GLSepi),GLS of the middle myocardium(GLSmid)and GLS of the endocardium(GLSendo)]and PSD were calculated.Receiver operating characteristic(ROC)analysis was performed to calculate the sensitivity and specificity of LV systolic dysfunction in T2DM patients with PVD.We calculated Pearson’s correlation coefficients between biochemical data,echocardiographic characteristics,and layer-specific GLS and PSD.RESULTS There were significant differences in GLSepi,GLSmid and GLSendo between normal controls,T2DM patients without PVD and T2DM patients with PVD(P<0.001).Trend tests revealed a ranking of normal controls>T2DM patients without PVD>T2DM patients with PVD in the absolute value of GLS(P<0.001).PSD differed significantly between the three groups,and the trend ranking was as follows:normal controls<T2DM patients without PVD<T2DM patients with PVD(P<0.001).ROC analysis revealed that the combination of layer-specific GLS and PSD had high diagnostic efficiency for detecting LV systolic dysfunction in T2DM patients with PVD.Lowdensity lipoprotein cholesterol was positively correlated with GLSepi,GLSmid and PSD(P<0.05),while LV ejection fraction was negatively correlated with GLSepi,GLSmid and GLSendo in T2DM patients with PVD(P<0.01).CONCLUSION PVD may aggravate the deterioration of LV systolic dysfunction in T2DM patients.Layer-specific GLS and PSD can be used to detect LV systolic dysfunction accurately and conveniently in T2DM patients with or without PVD.

Causal relationship between feelings and cognitive decline:An univariable and multivariable Mendelian randomization study

BACKGROUND While the impact of depression on cognition is well-documented,the relationship between feelings and cognition has received limited attention.AIM To explore the potential association between feelings and cognition with a twosample Mendelian randomization(MR)analysis.METHODS Our analysis utilized genome-wide association data on various feelings(fed-up feelings,n=453071;worrier/anxious feelings,n=450765;guilty feelings,n=45-0704;nervous feelings,n=450700;sensitivity/hurt feelings,n=449419;miserableness,n=454982;loneliness/isolation,n=455364;happiness,n=152348)in the European population and their impact on cognitive functions(intelligence,n=269867).Conducting a univariable MR(UVMR)analysis to assess the relationship between feelings and cognition.In this analysis,we applied the inverse variance weighting(IVW),weighted median,and MR Egger methods.Additionally,we performed sensitivity analysis(leave-one-out analysis),assessed heterogeneity(using MR-PRESSO and Cochran’s Q test),and conducted multiple validity test(employing MR-Egger regression).Subsequently,a multivariable MR(MVMR)analysis was employed to examine the impact of feelings on cognition.IVW served as the primary method in the multivariable analysis,complemented by median-based and MR-Egger methods.RESULTS In this study,UVMR indicated that sensitivity/hurt feelings may have a negative causal effect on cognition(OR=0.63,95%CI:0.43-0.92,P=0.017).After adjustment of other feelings using MVMR,a direct adverse causal effect on cognition was observed(OR_(MVMR)=0.39,95%CI:0.17-0.90,P_(MVMR)=0.027).While a potential increased risk of cognitive decline was observed for fed-up feelings in the UVMR analysis(ORUVMR=0.64,95%CI:0.42-0.97,PUVMR=0.037),this effect disappeared after adjusting for other feelings(OR_(MVMR)=1.42,95%CI:0.43-4.74,P_(MVMR)=0.569).These findings were generally consistent across MV-IVW,median-based,and MR-Egger analyses.MR-Egger regression revealed pleiotropy in the impact of worrier/anxious feelings on cognition,presenting a challenge in identifying the effect.Notably,this study did not demonstrate any significant impact of guilty feelings,nervous feelings,miserableness,or loneliness/isolation on cognition.Due to a limited number of instrumental variables for happiness,this study was unable to analyze the relationship between happiness and cognition.CONCLUSION This MR study finds that sensitivity/hurt feelings are associated with cognitive decline,while the link between worrier/anxious feelings and cognition remains inconclusive.Insufficient evidence supports direct associations between happiness,guilty feelings,nervous feelings,miserableness,loneliness/isolation,and cognition.

补饲胆固醇对高温环境下湖羊母羊繁殖性能和血清生化指标的影响

【目的】研究饲粮中添加胆固醇对中国南方夏季湖羊发情率、受胎率和血清生化指标的影响。【方法】选择108只体重相近(33.60 kg±1.86 kg)的健康湖羊母羊,其中30只湖羊在非高温季节(4月份,平均最高温度20.9℃±3.2℃)饲粮添加不同剂量胆固醇(0.1%和0.5%),ELISA方法检测血清中胆固醇含量的动态变化,筛选合适的胆固醇添加量;另外78只湖羊随机分成2组(对照组和试验组),试验期63 d(从同期发情处理第1天到自然发情后配种的第30天),在夏季高温季节(7-9月份,平均最高温度31.3℃±2.6℃),对照组饲喂基础饲粮,试验组饲喂添加0.1%胆固醇的基础饲粮,ELISA方法检测血清中雌二醇(E2)和孕酮(P4)水平,研究饲粮添加胆固醇对湖羊发情率、受胎率和血清生化指标的影响。【结果】非高温季节湖羊基础饲粮中分别添加0.1%和0.5%胆固醇,48 h后两组湖羊血清中胆固醇含量趋于一致,据此选择0.1%作为高温季节补饲胆固醇的添加剂量。夏季高温季节在补饲0.1%胆固醇的第3、30和63天均能显著提高湖羊血清中胆固醇含量(P<0.05);且试验组湖羊自然发情率(89.7%)高于对照组(82.1%)(P>0.05),受胎率(60.0%)显著高于对照组(46.9%)(P<0.05)。试验组湖羊总蛋白、球蛋白、白细胞介素-1和甘油三酯含量均显著高于对照组(P<0.05),而尿素氮含量显著下降(P<0.05)。【结论】夏季高温环境下,在湖羊母羊饲粮中添加0.1%胆固醇可有效提高其发情率和受胎率。

急诊护士突发公共卫生事件应对能力现状的多中心调查研究

目的了解急诊护士突发公共卫生事件应对经历及能力现状,为改善突发公共卫生事件应急能力提供参考依据。方法采用便利抽样法,于2023年3月—4月选取来自全国16个省市、119所医疗单位的1603名护士为调查对象,采用一般资料调查问卷、急诊护士突发公共卫生事件应急能力量表进行问卷调查。结果急诊护士突发公共卫生事件应急能力得分为[215.00(180.00,244.00)]分。多元线性回归分析结果显示,应急演练及培训经历、发热门诊轮转、性别、工作科室、年龄是急诊护士突发公共卫生事件应急能力的影响因素(P<0.05)。结论急诊护士突发公共卫生事件应急能力处于中等水平,针对突发公共卫生事件应急管理的不同阶段、根据不同层级护士的突发事件应对能力要求,按层次、按梯队开展持续、有计划的教育培训。

功率模块主动热控制技术研究综述

功率半导体器件是基于电力电子技术的电能变换、电力驱动等领域的核心,在新能源发电、交通运输以及航空航天等领域有广阔的应用前景,然而其发热原因造成的退化失效和可靠性等问题已成为其进一步发展的瓶颈,亟需探究有效的热管理方法,以提高其可靠性和使用寿命。在介绍功率模块的热管理方法的基础上,重点综述了其主动热管理方法的研究进展。依据控制参量的不同将其分为器件级、系统级和多参量的综合方法,并对各种方法进行了分析比对总结。最后提出了功率器件结温相关技术的发展趋势,进行了展望,以期为其后续研究应用提供参考。

基于时频域联合数据辅助MIMO均衡算法的多维度复用千公里级相干传输系统

基于少模光纤的模式复用系统利用单根纤芯中的正交模式实现各独立信道的并行传输,能够有效提升系统容量,解决单模光纤系统的容量危机.本文联合使用模式复用、波分复用与偏振复用技术,基于六模渐变型光纤实现了80个波长通道下32 Gbaud四模双偏振16阶正交振幅调制信号的1000 km相干传输,并在接收端使用时频域联合数据辅助8×8多输入多输出最小均方算法,以补偿由光纤传输导致的模式耦合及模间色散等线性损伤.与传统的纯时域或纯频域多输入输出最小均方均衡算法相比,该算法分别实现了57.1%的收敛速度提升与25.1%的收敛误差下降.经过1000 km少模光纤传输后,80通道每个模式及偏振态的误码率均低于开销为20%的软判决前向纠错编码阈值2.4×10^(-2),系统净速率达68.2 Tbit/s.

溶磷菌剂对玉米幼苗生长及根际土壤细菌群落结构和磷素形态的影响

以FD11(普瑞斯特氏菌Priestia sp.)和CH07(阿氏芽孢杆菌Bacillus aryabhattai)两种溶磷菌剂为试验材料,进行菌剂灌根盆栽试验,测定溶磷菌剂施用对玉米生长、根际土壤养分、土壤微生物特性及土壤不同形态磷含量的影响。结果表明,施用溶磷菌剂可改善根际土壤养分特征和微生物学特性,增加根际土壤的活性磷源含量,对玉米幼苗表现出良好的促生作用。与不施菌剂相比,FD11处理玉米幼苗的株高、茎粗、地上部干重分别增加31.88%、36.39%、104.00%;根际土壤碱解氮、有效磷、速效钾分别增加了21.90%、107.67%、5.77%。施用溶磷菌剂的土壤H_(2)O-Pi(Pi为无机磷)、NaHCO_(3)-P和NaOH-P含量增加,但HCl-Pi和Residual-P含量显著降低。施用溶磷菌剂改变了玉米根际土壤细菌的群落结构,在细菌属分类学水平上,不动杆菌属、假单胞菌属、海洋杆菌属、鞘氨醇单胞菌属、节杆菌属等功能细菌的相对丰度显著增加。综合以上分析,施用溶磷菌剂显著提高了土壤速效养分含量,改变了根际土壤细菌的群落结构,增加了活性磷源的含量,从而改善了土壤肥力,促进玉米根系发育和幼苗生长。此项研究可为新型微生物菌肥的研发提供理论依据。

9YQ-2200S型秸秆方捆打捆机设计与试验

【目的】饲料化是我国主要农作物秸秆综合利用的重点发展方向,为提高田间饲用秸秆的回收效率和质量,解决常规小型饲草方捆机适用范围小、作业效率低、揉碎质量差和不能有效除尘的问题。【方法】基于秸秆切碎揉搓、压缩打捆基本理论,采用理论分析计算和SolidWorks三维设计方法,设计了1种适用于田间玉米、小麦、水稻、棉花等作物秸秆的9YQ-2200S型秸秆方捆打捆机,开展了样机试制与改进,并针对玉米秸秆进行了田间试验。【结果】样机的收净率为90.3%,成捆率为100%,规则草捆率为100%,抗摔率为91.66%,草捆密度为119.67 kg/m^(3),作业小时生产率为3.36 t/h,各项作业指标均符合相关标准要求,作业性能良好。【结论】该机可实现作物秸秆自动捡拾(切割)、粉碎、揉丝、除尘、高密度压缩和自动绳打捆一体化作业。本研究可为秸秆方捆打捆机的设计与改进提供参考。

盐碱地花生棉花等幅间作对花生光合特性及产量效益的影响

为充分利用盐碱地土地及温光热资源,明确盐碱地花生/棉花等幅间作种植模式下,不同垄行位置对花生光合产物积累和光合特性的影响,提高盐碱地农业生产综合效益,在田间条件下,采用花生/棉花6∶4等幅间作周年轮作高效种植模式,研究花生带不同垄行位置对其光合产物积累、光照强度、叶绿素荧光特性等的影响及产量和效益的变化。结果表明:(1)盐碱地花生/棉花等幅间作种植模式较花生单作可显著增加花生主茎高、侧枝长、功能叶片非光化学荧光淬灭系数(NPQ),显著降低生物量、相对叶绿素含量(SPAD)、叶面积系数(LAI)、净光合速率(P_(n))、蒸腾速率(T_(r)),产量明显降低;间作棉花果枝数、单株铃数、铃重和产量均明显提高,间作系统综合经济效益显著提高。(2)间作系统中花生带两边垄行(IP1、IP3)、单作花生和单作棉花的冠层光照强度峰值均出现在12:00,但中间垄行(IP2)花生冠层辐射强度峰值滞后到14:00,且间作花生带两边垄行冠层光照度显著低于花生单作处理。(3)间作系统中,花生花针期后垄行位置对光合效率的影响显著,中间垄行SPAD、LAI和NPQ值均显著高于两边垄行,而两边垄行间无明显差异;但P_(n)、气孔导度(G_(s))和T_(r)受试点和垄行位置影响显著,其中东营市试点花生各生育期的各垄行位置间均无显著差异,而高唐县试点间作花生功能叶P_(n)、G_(s)和T_(r)均显著低于单作处理,且间作处理的IP2垄行显著高于IP1、IP3两边垄行。(4)两试点间作棉花和花生产量低于其单作处理,但综合效益较传统植棉收益分别提高11.94%(东营)和27.08%(高唐),间作系统土地当量比大于1.02。

干旱地区砂质土壤棉花苗床土层构建装置的研制

针对新疆南部砂质土壤“干播湿出”棉花种植模式下,春季耕整地连续作业存在作业阻力大、苗床整不平、压不实,严重影响棉花出苗率的问题,该研究设计了一种适合砂质土壤的苗床整备装置,可一次完成碎土、平土、灭茬、镇压、耱地等作业。采用分段平土装置代替耙组进行联合整备作业,分段平土装置为两段式,首段刮土板呈“W”型对称分布,通过刮土板角度对土壤流动性、平整度和作业阻力的运动分析,确定当刮土板倾斜角为45°时碎土和平土效果最好;台架试验结果表明,镇压辊直径400 mm、碎土辊直径400 mm时综合评分最高。样机性能对比试验结果表明:较耙片式联合整地机综合工作阻力降低8.67%,作业效率提升6.6%;地表平整度标准差为11 mm;耕深稳定性变异系数为9.2%,碎土率为92.3%,表面未见秸秆残膜,分段平土装置能有效碎土、平土、灭茬;二次整备后浮动式土层构建装置能有效压实土壤构建出“上虚下实”土层结构,0~5cm播种土层平均土壤紧实度为169 kPa,适合砂质土壤精量铺膜播种作业和棉花生长,较对比机型种膜错位率下降62.5%,出苗率高于对比机型4.8%。研究结果可为砂质土壤苗床整地机研发与改进提供参考。

新辅助免疫化疗对比新辅助化疗用于局部进展期胃癌的临床疗效分析

目的分析新辅助免疫化疗(neoadjuvant immunochemotherapy,NAIC)和新辅助化疗(neoadjuvant chemotherapy,NAC)用于局部进展期胃癌的临床疗效,探究疗效相关临床病理特征。方法本研究采用回顾性队列研究设计方案,收集2017年1月至2023年7月陆军特色医学中心(大坪医院)和中国医科大学第一附属医院共243例局部进展期胃癌患者的临床资料,通过纳入排除标准和倾向性评分匹配(propensity score matching,PSM)均衡2组间混杂因素后,分析2种新辅助治疗方案的肿瘤病理缓解率和安全性,并对临床病理特征进行亚组分析寻找与疗效相关的临床病理特征。结果对两组患者采用PSM平衡两组基线特征后,每组各53名受试者纳入分析。在肿瘤病理退缩方面,NAIC组(13名,25%)的病理完全缓解(pathologic complete response,pCR)率显著高于NAC组(2名,3.8%,P<0.05)。在主要病理学缓解(major pathological response,MPR)方面也观察到类似的结果,NAIC组有23名(43%)患者实现了MPR,而NAC组有9名(17%)患者实现MPR(P<0.05)。安全性方面,NAIC组和NAC组的任何等级TRAEs发生率相当(分别为96.2%和96.2%)。基于肿瘤病理退缩的探索性亚组分析发现,符合年龄<65岁、男性、AJCC分期的Ⅲ~ⅣA期、腺癌、高-中分化、肠型胃癌、T分期的T3~T4分期和N分期的N2~N3分期等临床病理特征的胃癌患者,更可能获益于新辅助免疫化疗。结论局部进展期胃癌接受新辅助免疫化疗的病理学缓解率优于新辅助化疗,不良反应发生率相当。

陕产附子炮制前后急性毒性及强心作用研究

目的:考察陕产附子炮制前后的急性毒性和强心作用。方法:通过对生附子LD_(50)的测定和自制黑顺片最大耐受量实验比较生附子和自制黑顺片的急性毒性;通过复制慢性心衰大鼠模型,检测大鼠体质量、心脏指数、血流动力学指标,检测血清BNP及cTnI含量以及组织病理学检查比较其强心作用。结果:生附子的半数致死量(LD_(50))为9.45 g/kg,黑顺片的最大耐受量为27.39 g/kg,经炮制后毒性降低。与模型组相比,自制黑顺片组大鼠症状及体征均有改善,能够降低心脏指数、左心室指数降低和左心室舒张压(LVEDP),升高左心室收缩压(LVSP)、收缩期左心室内压上升最大变化速率(+dp/dt_(max))和舒张期左心室内压下降最大变化速率(-dp/dt_(max))(P<0.05,P<0.01);降低血清中脑钠肽(BNP)、心肌肌钙蛋白I(cTnI)含量(P<0.05);心肌纤维排列相对紧密,胞核清晰可见,但有一定断裂,其中有少量空泡存在,未发现炎性细胞浸润,各指标有明显差异。生附子组各指标有一定程度的改善,但差异无统计学意义(P>0.05)。结论:生附子经炮制后,毒性降低,起到减毒的作用;自制黑顺片较生附子强心作用有显著增强。

2个中晚熟黄绿色免套袋苹果新品种在昭通引种表现及栽培技术要点

“中田”“青林”是自日本引进的黄绿色中晚熟苹果新品种,“中田”果实短圆锥形,平均单果质量228.12 g,果面黄色,果肉黄白色,肉质细嫩、松脆,酸甜适度,可溶性固形物16.92%,带皮硬度7.28 kg/cm^(2),可滴定酸含量0.41%,维生素C含量6.86 mg/100 g,果实成熟期8月下旬;“青林”果实近圆形,平均单果质量217.34 g,果面采收时绿黄色,后逐步变黄色,果肉黄白色,肉质细嫩、脆,酸甜适度,可溶性固形物16.63%,带皮硬度9.82 kg/cm^(2),可滴定酸含量0.32%,维生素C含量6.48 mg/100 g,果实成熟期为9月中下旬。2个品种鲜食品质佳,常温条件下果实可保存35~40 d。“中田”“青林”2个苹果新品种,免套袋栽培性状良好,抗病性有所提高,可供应9—10月中高端市场,适宜在云南省昭通市昭阳区及气候类似地区作为特色品种进行推广种植。

某锂矿低温熟化—水浸提锂和提纯高纯石英新工艺

皖南低品位热液蚀变型锂矿Li_(2)O含量0.21%,属于新类型锂矿资源,主要矿物成分有长石、石英、白云母、绿泥石,锂主要以晶格形式赋存于锂绿泥石中。新工艺采用-1 mm原矿筛分,+0.425 mm粒级进入磨矿,制备-0.425+0.074 mm合格粒级。脱泥后合格粒级产品经磁选得云母精矿,非磁性物浮选得到长石精矿和石英精矿,长石精矿和云母精矿分别进行提锂,石英精矿进行酸处理提纯得高纯石英。将原矿筛出的-0.074 mm细泥、再磨产生的-0.074 mm细泥、合格粒级搅拌擦洗产生的-0.074 mm细泥,合并为总泥进行后续提锂。试验结果表明:总泥、云母、长石分别采用低温熟化—水浸工艺提锂,累计得Li_(2)O浸出率88.78%,酸浸后石英精矿含SiO_(2)99.942%,Fe 4.38 mg/kg,达到高纯石英低端产品要求,提锂后的长石和云母浸渣产率54.48%,可用于建筑材料。浸出前后样品的XRD和SEM分析表明,硫酸低温熟化破坏了锂绿泥石晶格结构,H+代替锂绿泥石中Li+,将锂释放出来。新工艺降低了磨矿成本,锂浸出率高,兼顾云母、石英、长石的回收,减少了尾矿排放,提高了低品位热液蚀变型锂矿的综合利用水平。

白细胞介素-33/ST2信号通路调控辅助T细胞/调节性T细胞免疫平衡参与慢性阻塞性肺疾病发病机制分析

目的探究基于白细胞介素-33(IL-33)/ST2信号通路激活树突状细胞(DCs)成熟,调控辅助T细胞17(Th17)/调节性T细胞(Treg)免疫平衡参与小鼠慢性阻塞性肺疾病(COPD)发病的临床机制。方法选择36只SPF级C57BL/6健康小鼠,随机选取12只作为健康对照组,其余小鼠用于制作COPD模型,采用香烟烟雾刺激和气道内注入脂多糖的方式建立COPD模型,以小鼠肺部压力-容积曲线移动或弹性程度降低为造模成功,将模型小鼠按照随机数字表法分为COPD组(n=12)和IL-33抗体干预组(n=12)。于造模开始第3周时为IL-33抗体组小鼠腹腔注射IL-33抗体,健康对照组和COPD组小鼠注射同等剂量生理盐水,于造模开始第28天在小鼠清醒状态下采集三组小鼠的内眦静脉从血液,使用PBS液冲洗三组小鼠右肺组织并收集支气管肺泡灌洗液(BALF),比较三组小鼠外周血、BALF中DCs、IL-33表达情况差异,采集肺组织甲醛固定后染色处理,光镜下观察肺组织病理变化。结果COPD组小鼠外周血、BALF中Th17/Treg比值均高于健康对照组和IL-33抗体干预组,差异有统计学意义(P<0.05);COPD组小鼠外周血、BALF中DCs成熟标志物CD80、CD86水平均高于健康对照组和IL-33抗体组,主要组织相容性复合体(MHC)-Ⅱ低于健康对照组和IL-33抗体组,差异有统计学意义(P<0.05);RT-PCR分析显示,COPD组小鼠IL-33灰度值高于健康对照组和IL-33抗体干预组,差异有统计学意义(P<0.05);三组小鼠气道炎症病理差异较大。结论调控IL-33/ST2信号通路可以激活DCs细胞成熟,恢复COPD小鼠Th17/Treg平衡,改善COPD小鼠炎症指标水平。

全球电影贸易网络时空格局演化特征分析

基于联合国商品贸易统计数据库,利用复杂网络分析方法,探讨2004—2019年全球电影贸易网络时空格局演化特征。研究发现:1)全球电影贸易网络节点数量、贸易关系流和平均加权度持续增加,各国或地区在全球电影贸易网络中的联系越来越密切。贸易网络的可达性和通达性良好,具有小世界特征和无标度性质。2)贸易网络的拓扑结构从以欧洲、美国为双核心演变为以欧洲、亚洲和美国的“多点开花”式多极结构,以往全球电影贸易网络中“自西向东、由北往南”的空间格局正在变化,亚洲国家在全球电影贸易中增长显著,中国成为亚洲区域性电影贸易中心。3)网络核心—边缘结构越来越明显,电影贸易出现较为明显的等级层次性。全球电影贸易网络表现出全球化和区域化共存的趋势,区域化已成为全球电影贸易网络演化的重要趋势之一。4)全球电影贸易网络可划分为4个主要社团,其分布呈空间的连续性,社团结构的存在促进电影贸易网络的稳定和发展。