拷贝数变异(Copy number variation,CNV)作为基因组结构变异(Structural variation,SV)的重要组成部分,在物种表型变异、疾病易感性评估、物种演化等方面起着重要作用。目前,高通量、高分辨率的全基因组CNV研究已广泛应用到各种家畜中,...拷贝数变异(Copy number variation,CNV)作为基因组结构变异(Structural variation,SV)的重要组成部分,在物种表型变异、疾病易感性评估、物种演化等方面起着重要作用。目前,高通量、高分辨率的全基因组CNV研究已广泛应用到各种家畜中,并成为全基因组关联性分析(Genome-wide association analysis,GWAS)研究复杂性状的重要分子标记。此外,CNV还为群体遗传结构分析提供新的视角。因此,本文从定义、形成机制、分布特点、检测方法、遗传效应和牛Y染色体CNV等方面对牛全基因组CNV研究进行了较为全面的介绍与阐述,提出全基因组CNV研究所面临的一些问题,并对发展前景做了简单展望,以期为今后牛全基因组CNV的研究提供指导信息。展开更多
To study the effects of 17beta-estradiol （E2 ） and progesterone （P4） levels on superovulatory response and recipients conception rate, the E2 and P4 levels of embryo donor （Borderdale） and recipient ewes （Inner...To study the effects of 17beta-estradiol （E2 ） and progesterone （P4） levels on superovulatory response and recipients conception rate, the E2 and P4 levels of embryo donor （Borderdale） and recipient ewes （Inner Mongolian sheep） were determined during multiple ovulation and em- bryo transfer （MOET）. The results showed that the E2 level increased from the day of initiation by CIDR （Controlled internal drug release devices） treatment and decreased to 3.18± 1.32 pg/ml on the day of superovulatory estrus. On the day of embryo collection, the ewes having higher levels of estrogenic peak showed better superovulatory response than the other groups having comparatively lower levels. On the day of initiation of super- ovulatory treatment, the ewes having lower levels of P4 showed better superovulatory response than those having higher levels of P4. The E2 level of the early abortion group increased from Day 0 to Day 45, while that of two pregnant recipient groups came down from Day 0 to Day 30. The ser- um P4 level of the early abortion group was significantly lower than that of the pregnant recipient groups on Day 45 （ P 〈 0.05）, but no significant difference was found between the two pregnant groups after Day 30. Therefore, the levels of E2 and P, on definite time affect superovulatory re- sponse of donors and conception rate of recipients during MOET.展开更多
文摘拷贝数变异(Copy number variation,CNV)作为基因组结构变异(Structural variation,SV)的重要组成部分,在物种表型变异、疾病易感性评估、物种演化等方面起着重要作用。目前,高通量、高分辨率的全基因组CNV研究已广泛应用到各种家畜中,并成为全基因组关联性分析(Genome-wide association analysis,GWAS)研究复杂性状的重要分子标记。此外,CNV还为群体遗传结构分析提供新的视角。因此,本文从定义、形成机制、分布特点、检测方法、遗传效应和牛Y染色体CNV等方面对牛全基因组CNV研究进行了较为全面的介绍与阐述,提出全基因组CNV研究所面临的一些问题,并对发展前景做了简单展望,以期为今后牛全基因组CNV的研究提供指导信息。
基金funded by Agricultural Research Institute"948"Program of China(2000-2005)
文摘To study the effects of 17beta-estradiol （E2 ） and progesterone （P4） levels on superovulatory response and recipients conception rate, the E2 and P4 levels of embryo donor （Borderdale） and recipient ewes （Inner Mongolian sheep） were determined during multiple ovulation and em- bryo transfer （MOET）. The results showed that the E2 level increased from the day of initiation by CIDR （Controlled internal drug release devices） treatment and decreased to 3.18± 1.32 pg/ml on the day of superovulatory estrus. On the day of embryo collection, the ewes having higher levels of estrogenic peak showed better superovulatory response than the other groups having comparatively lower levels. On the day of initiation of super- ovulatory treatment, the ewes having lower levels of P4 showed better superovulatory response than those having higher levels of P4. The E2 level of the early abortion group increased from Day 0 to Day 45, while that of two pregnant recipient groups came down from Day 0 to Day 30. The ser- um P4 level of the early abortion group was significantly lower than that of the pregnant recipient groups on Day 45 （ P 〈 0.05）, but no significant difference was found between the two pregnant groups after Day 30. Therefore, the levels of E2 and P, on definite time affect superovulatory re- sponse of donors and conception rate of recipients during MOET.
