Rice(Oryza sativa L.)is grown nearly worldwide and provides the staple food for more than half of the global population(Luo et al,2017).The genomes of several cultivated rice varieties including Nipponbare(NPB)(Kawaha...Rice(Oryza sativa L.)is grown nearly worldwide and provides the staple food for more than half of the global population(Luo et al,2017).The genomes of several cultivated rice varieties including Nipponbare(NPB)(Kawahara et al,2013;Sakai et al,2013),IR64(Tanaka et al,2020),93-11(Zhang et al,2018)and R498(Du et al,2017)at chromosome level,and Minghui 63 and Zhenshan 97(Zhang et al,2016)at scaffold level have been assembled,annotated and released,among which the R498 and NPB genomes are widely used as reference genomes in rice research.However,there are thousands of rice cultivars,landraces and wild rice varieties in the world with dramatically different genetic backgrounds,and the genomes of native rice varieties in South China,which is one of the major rice production areas in China,have not been de novo assembled.Huajingxian 74(HJX74)is an indica rice variety bred in South China Agricultural University,Guangdong Province with widely environmental adaptability and high yield(www.ricedata.cn/variety/varis/602548.htm).HJX74 exhibits significant phenotypic and genetic differences from those varieties whose whole genomes have been properly sequenced and assembled(Fig.1).展开更多
The aim of this paper was to investigate the effects of resistin on human umbilical vein endothelial cells(HUVECs),and to explore its role and mechanism of action in atherosclerosis.HUVECs were incubated with recombin...The aim of this paper was to investigate the effects of resistin on human umbilical vein endothelial cells(HUVECs),and to explore its role and mechanism of action in atherosclerosis.HUVECs were incubated with recombinant human resistin(0,50,100 ng/mL)for 24 h.ICAM-1,VCAM-1 and reactive oxygen species(ROS)were assayed by flow cytometer.ET-1,eNOS and iNOS mRNA expression were measured by semi-quantitative RT-PCR.Incubation of HUVECs with resistin resulted in an increase in ICAM-1 expression and ET-1 mRNA expression.However,resistin had no effect on VCAM-1 expression and ROS release.eNOS and iNOS mRNA expression were not altered by resistin stimulation.Adipokine resistin exerted a direct effect in promoting HUVEC dysfunction by promoting ICAM-1 and ET-1 expression.These data suggest that adipocyteendothelium cross-talk might play an important role in the pathogenesis of cardiovascular disease in diabetes mellitus.展开更多
基金the National Key Research and Development Program of China(Grant No.2016YFD0100406)National College Students Innovation and Entrepreneurship Foundation of China(Grant No.201910564054)+1 种基金National Natural Science Foundation of China(Grant Nos.91735304 and 31622041)Special Project for Leading Talents in Innovation of Science and Technology of Guangdong Province,China(Grant No.2016TX03N224)。
文摘Rice(Oryza sativa L.)is grown nearly worldwide and provides the staple food for more than half of the global population(Luo et al,2017).The genomes of several cultivated rice varieties including Nipponbare(NPB)(Kawahara et al,2013;Sakai et al,2013),IR64(Tanaka et al,2020),93-11(Zhang et al,2018)and R498(Du et al,2017)at chromosome level,and Minghui 63 and Zhenshan 97(Zhang et al,2016)at scaffold level have been assembled,annotated and released,among which the R498 and NPB genomes are widely used as reference genomes in rice research.However,there are thousands of rice cultivars,landraces and wild rice varieties in the world with dramatically different genetic backgrounds,and the genomes of native rice varieties in South China,which is one of the major rice production areas in China,have not been de novo assembled.Huajingxian 74(HJX74)is an indica rice variety bred in South China Agricultural University,Guangdong Province with widely environmental adaptability and high yield(www.ricedata.cn/variety/varis/602548.htm).HJX74 exhibits significant phenotypic and genetic differences from those varieties whose whole genomes have been properly sequenced and assembled(Fig.1).
基金This study was supported by the Foundation of the Ministry of Health of China(No.000099009)Foundation of Ministry of Education of China(No.002000001).
文摘The aim of this paper was to investigate the effects of resistin on human umbilical vein endothelial cells(HUVECs),and to explore its role and mechanism of action in atherosclerosis.HUVECs were incubated with recombinant human resistin(0,50,100 ng/mL)for 24 h.ICAM-1,VCAM-1 and reactive oxygen species(ROS)were assayed by flow cytometer.ET-1,eNOS and iNOS mRNA expression were measured by semi-quantitative RT-PCR.Incubation of HUVECs with resistin resulted in an increase in ICAM-1 expression and ET-1 mRNA expression.However,resistin had no effect on VCAM-1 expression and ROS release.eNOS and iNOS mRNA expression were not altered by resistin stimulation.Adipokine resistin exerted a direct effect in promoting HUVEC dysfunction by promoting ICAM-1 and ET-1 expression.These data suggest that adipocyteendothelium cross-talk might play an important role in the pathogenesis of cardiovascular disease in diabetes mellitus.
