目的 :分析导致阿尔兹海默症合并肺部感染患者死亡的危险因素。方法 :对2014年10月至2017年1月期间在南京鼓楼医院住院治疗的56例阿尔兹海默症合并肺部感染患者的临床资料进行回顾性分析。根据临床结局将其分为存活组和死亡组。采用单...目的 :分析导致阿尔兹海默症合并肺部感染患者死亡的危险因素。方法 :对2014年10月至2017年1月期间在南京鼓楼医院住院治疗的56例阿尔兹海默症合并肺部感染患者的临床资料进行回顾性分析。根据临床结局将其分为存活组和死亡组。采用单因素分析法和多因素回归分析法找出导致阿尔兹海默症合并肺部感染患者死亡的危险因素。结果:死亡组患者中体温≥38.5℃、存在胸腔积液、存在下肢水肿、留置有鼻胃管、留置有导尿管、留置有深静脉导管、接受过呼吸机治疗、CRP的水平≥100 mg/L、白蛋白的水平≤30 g/L、血氧分压<60 mm Hg、血二氧化碳分压≥50 mm Hg患者的比率均高于存活组患者(P<0.05)。死亡组患者中感染鲍曼不动杆菌患者的比率高于存活组患者(P<0.05)。进行多因素Logistic回归分析的结果显示,BMI较低、血氧分压<60 mm Hg、血二氧化碳分压≥50 mm Hg、感染鲍曼不动杆菌是导致阿尔兹海默症合并肺部感染患者死亡的独立危险因素(P<0.05)。结论 :导致阿尔兹海默症合并肺部感染患者死亡的危险因素主要包括其BMI较低、血氧分压<60 mm Hg、血二氧化碳分压≥50 mm Hg、感染鲍曼不动杆菌等。临床医生应对上述情况予以关注。展开更多
Optimal conditions for protoplasts formation of marine Actinomycete sp. isolate M048 were described, dense and disperse mycelia were cultured in SGGP medium, 0.5% glycine, lysozyme exposure （2 mg/cm^3, 37 ℃, 40 min...Optimal conditions for protoplasts formation of marine Actinomycete sp. isolate M048 were described, dense and disperse mycelia were cultured in SGGP medium, 0.5% glycine, lysozyme exposure （2 mg/cm^3, 37 ℃, 40 min）, and the concentration of sucrose in protoplast buffer was 0.4 mol/dm^3 for keeping the balance of osmotic pressure. Using PEG-mediated pmtoplasts transformarion, the transformation frequency was 89 transformants per microgramme of pIJ702. Meanwhile, an effective transformation procedure was established based on intergeneric conjugation from E. coli ET12567 （pUZS002） using shuttle vectors pPM801, pPM803 and a φC31-derived integration vector pIJ8600 containing onT and attP fragments. Transformation frequencies were 5.30 ×10^-4 ±0.26 ×10^-4 , 8.92 ×10^-4 ±0. 19 ×10^-4 and 6.38 ×10^-5 ±0.41×10^-5 respectively. Further, the heterologous expression of the allophycocyanin gene （apc） in the strain M048 was used to demonstrate this transformation system. SDS - PAGE and Western blot analysis confirmed the expression of recombinant APC （rAPC）.展开更多
Green tides have occurred in the Yellow Sea successively from 2007 to 2011. Genetic analysis of the 5-year green-tide-forming algae needs to be performed to determine the source of the biomass and understand the mecha...Green tides have occurred in the Yellow Sea successively from 2007 to 2011. Genetic analysis of the 5-year green-tide-forming algae needs to be performed to determine the source of the biomass and understand the mechanism of the green tide blooms. In this study, free-floating green algae were collected at different sites in the Yellow Sea in 2010 and 2011. Data on 182 free-floating samples and 155 attached Ulva samples from previous studies on the Yellow Sea green tides from 2007 to 2009 were also taken into consideration. Morphology observation and molecular phylogenetic analyses indicated that the Yellow Sea green tides were dominated by a single species, Ulva prolifera, from 2007 to 2011. Considering that at least five Ulva species inhabit the north coast of China, the unialgal composition of the green tides implied that (1) there may be some special physiology and propagation pathways of U. prolifera for its rapid expansion, (2) the mechanisms of the Yellow Sea green tide formation were similar for the last five years, and (3) the intra-species genetic variation and population structure of U. prolifera need to be studied to determine the exact origin of the bloom-forming biomass.展开更多
文摘目的 :分析导致阿尔兹海默症合并肺部感染患者死亡的危险因素。方法 :对2014年10月至2017年1月期间在南京鼓楼医院住院治疗的56例阿尔兹海默症合并肺部感染患者的临床资料进行回顾性分析。根据临床结局将其分为存活组和死亡组。采用单因素分析法和多因素回归分析法找出导致阿尔兹海默症合并肺部感染患者死亡的危险因素。结果:死亡组患者中体温≥38.5℃、存在胸腔积液、存在下肢水肿、留置有鼻胃管、留置有导尿管、留置有深静脉导管、接受过呼吸机治疗、CRP的水平≥100 mg/L、白蛋白的水平≤30 g/L、血氧分压<60 mm Hg、血二氧化碳分压≥50 mm Hg患者的比率均高于存活组患者(P<0.05)。死亡组患者中感染鲍曼不动杆菌患者的比率高于存活组患者(P<0.05)。进行多因素Logistic回归分析的结果显示,BMI较低、血氧分压<60 mm Hg、血二氧化碳分压≥50 mm Hg、感染鲍曼不动杆菌是导致阿尔兹海默症合并肺部感染患者死亡的独立危险因素(P<0.05)。结论 :导致阿尔兹海默症合并肺部感染患者死亡的危险因素主要包括其BMI较低、血氧分压<60 mm Hg、血二氧化碳分压≥50 mm Hg、感染鲍曼不动杆菌等。临床医生应对上述情况予以关注。
文摘Optimal conditions for protoplasts formation of marine Actinomycete sp. isolate M048 were described, dense and disperse mycelia were cultured in SGGP medium, 0.5% glycine, lysozyme exposure （2 mg/cm^3, 37 ℃, 40 min）, and the concentration of sucrose in protoplast buffer was 0.4 mol/dm^3 for keeping the balance of osmotic pressure. Using PEG-mediated pmtoplasts transformarion, the transformation frequency was 89 transformants per microgramme of pIJ702. Meanwhile, an effective transformation procedure was established based on intergeneric conjugation from E. coli ET12567 （pUZS002） using shuttle vectors pPM801, pPM803 and a φC31-derived integration vector pIJ8600 containing onT and attP fragments. Transformation frequencies were 5.30 ×10^-4 ±0.26 ×10^-4 , 8.92 ×10^-4 ±0. 19 ×10^-4 and 6.38 ×10^-5 ±0.41×10^-5 respectively. Further, the heterologous expression of the allophycocyanin gene （apc） in the strain M048 was used to demonstrate this transformation system. SDS - PAGE and Western blot analysis confirmed the expression of recombinant APC （rAPC）.
基金supported by the Public Science and Technology Research Funds Projects of Ocean (201205010)Promotive Research Fund for Excellent Young and Middle-aged Scientists of Shandong Province(2010BSB02009)the National Science & Technology Pillar Program of China (2008BAC49B01)
文摘Green tides have occurred in the Yellow Sea successively from 2007 to 2011. Genetic analysis of the 5-year green-tide-forming algae needs to be performed to determine the source of the biomass and understand the mechanism of the green tide blooms. In this study, free-floating green algae were collected at different sites in the Yellow Sea in 2010 and 2011. Data on 182 free-floating samples and 155 attached Ulva samples from previous studies on the Yellow Sea green tides from 2007 to 2009 were also taken into consideration. Morphology observation and molecular phylogenetic analyses indicated that the Yellow Sea green tides were dominated by a single species, Ulva prolifera, from 2007 to 2011. Considering that at least five Ulva species inhabit the north coast of China, the unialgal composition of the green tides implied that (1) there may be some special physiology and propagation pathways of U. prolifera for its rapid expansion, (2) the mechanisms of the Yellow Sea green tide formation were similar for the last five years, and (3) the intra-species genetic variation and population structure of U. prolifera need to be studied to determine the exact origin of the bloom-forming biomass.
