【目的】为了深入了解月面建造过程中月壤的力学特性,评估月基装备性能,优化月面建造作业,开展离散元法(discrete element method,DEM)在月壤接触力学领域的应用调查研究,旨在利用离散元法为未来月球基地建设提供理论指导和技术支持。...【目的】为了深入了解月面建造过程中月壤的力学特性,评估月基装备性能,优化月面建造作业,开展离散元法(discrete element method,DEM)在月壤接触力学领域的应用调查研究,旨在利用离散元法为未来月球基地建设提供理论指导和技术支持。【研究现状】基于地质勘探、资源采集及运输、建造作业等月面原位建造任务场景,分析月壤颗粒的建模与参数标定,介绍月基装备与月壤的接触作用研究现状,概述离散元法在钻-壤作用模拟、铲-壤作用模拟、轮-壤作用模拟及足-壤作用模拟中的应用,探讨基于离散元法的天然月基承载力分析。【结论与展望】提出降低几何模型复杂度、优化粒间接触模型及参数是提高宏观尺度月壤离散元建模精度以应对大规模月面建造场景的有效方法。认为利用离散元法进行铲挖式月壤采集装置及足式月球车设计在未来可以为月面建造提供可靠的技术支持。面向月面建造,离散元法将在水冰资源利用、建筑物月面承载力分析等方面提供科学依据。展开更多
OBJECTIVE To investigate the regulatory effects of icariin(ICA)on cardiac micro⁃vascular endothelial cells(CMEC)after oxygenglucose deprivation reperfusion(OGD/R)injury.METHODS CMEC were subjected to OGD/R treatment t...OBJECTIVE To investigate the regulatory effects of icariin(ICA)on cardiac micro⁃vascular endothelial cells(CMEC)after oxygenglucose deprivation reperfusion(OGD/R)injury.METHODS CMEC were subjected to OGD/R treatment to construct a myocardial ischemiareperfusion model,and were divided into normal,model,low(10μmol·L^(-1)),medium(20μmol·L^(-1))and high(40μmol·L^(-1))ICA group,and high ICA+inhibitor group(40μmol·L^(-1)+20 nmol·L^(-1)).CCK-8 assay was used to assess the protective ability of ICA against CMEC,and cell migration assay and tube-formation assay were used to detect the migration and generation ability of CMEC.The TCMSP database,Swiss-Target database and literature mining methods were used to col⁃lect ICA-related targets,the GeneCards data⁃base was used to collect target genes related to myocardial ischemia/reperfusion,and Cytoscape 3.8.0 software was used to construct a"drug-tar⁃get-disease"network.The potential targets were imported into STRING 11.5 database to obtain the PPI network.GO and KEGG enrichment analyses were performed on the potential targets using the DAVID database.Molecular docking was performed using AutoDock-vina 1.1.2 soft⁃ware.Western blot detected the expression of related proteins.RESULTS After CMEC was subjected to OGD/R treatment,ICA had a protec⁃tive effect at 10^(-1)60μmol·L^(-1);the results of the cell migration assay showed that each group of ICA could promote the migratory effect of CMEC(P<0.01,P<0.01);and the results of tube-for⁃mation assay showed that each group of ICA could significantly promote the generation of branches(P<0.01)and the capillary length exten⁃sion(P<0.05).Network pharmacology collected a total of 23 ICA action targets,1500 disease tar⁃gets and 12 key targets.GO function enrichment analysis found 85 results.KEGG pathway enrich⁃ment analysis found 53 results,involving AGERAGE signaling pathway,sphingolipid signaling pathway and VEGF signaling pathway.Molecu⁃lar docking results showed that ICA had better binding with core targets PRKCB,PRKCA and PTGS2.Western blot results showed that ICA could regulate the expression of PRKCB,PRKCA and PTGS2 proteins.The results of cell migra⁃tion assay,tube-formation assay and protein expression were reversed after addition of PKC inhibitor.CONCLUSION The potential mecha⁃nism of action of ICA against myocardial isch⁃emia-reperfusion injury may be related to the reg⁃ulation of processes such as CMEC migration and angiogenesis,and it functions through the key target gene PKC.展开更多
文摘【目的】为了深入了解月面建造过程中月壤的力学特性,评估月基装备性能,优化月面建造作业,开展离散元法(discrete element method,DEM)在月壤接触力学领域的应用调查研究,旨在利用离散元法为未来月球基地建设提供理论指导和技术支持。【研究现状】基于地质勘探、资源采集及运输、建造作业等月面原位建造任务场景,分析月壤颗粒的建模与参数标定,介绍月基装备与月壤的接触作用研究现状,概述离散元法在钻-壤作用模拟、铲-壤作用模拟、轮-壤作用模拟及足-壤作用模拟中的应用,探讨基于离散元法的天然月基承载力分析。【结论与展望】提出降低几何模型复杂度、优化粒间接触模型及参数是提高宏观尺度月壤离散元建模精度以应对大规模月面建造场景的有效方法。认为利用离散元法进行铲挖式月壤采集装置及足式月球车设计在未来可以为月面建造提供可靠的技术支持。面向月面建造,离散元法将在水冰资源利用、建筑物月面承载力分析等方面提供科学依据。
基金National Natural Science Foundation of China(82030124)National Natural Science Foundation of China(82174015)Science and Technology Innovation Project of China Academy of Traditional Chinese Medicine(CI2021A04609)。
文摘OBJECTIVE To investigate the regulatory effects of icariin(ICA)on cardiac micro⁃vascular endothelial cells(CMEC)after oxygenglucose deprivation reperfusion(OGD/R)injury.METHODS CMEC were subjected to OGD/R treatment to construct a myocardial ischemiareperfusion model,and were divided into normal,model,low(10μmol·L^(-1)),medium(20μmol·L^(-1))and high(40μmol·L^(-1))ICA group,and high ICA+inhibitor group(40μmol·L^(-1)+20 nmol·L^(-1)).CCK-8 assay was used to assess the protective ability of ICA against CMEC,and cell migration assay and tube-formation assay were used to detect the migration and generation ability of CMEC.The TCMSP database,Swiss-Target database and literature mining methods were used to col⁃lect ICA-related targets,the GeneCards data⁃base was used to collect target genes related to myocardial ischemia/reperfusion,and Cytoscape 3.8.0 software was used to construct a"drug-tar⁃get-disease"network.The potential targets were imported into STRING 11.5 database to obtain the PPI network.GO and KEGG enrichment analyses were performed on the potential targets using the DAVID database.Molecular docking was performed using AutoDock-vina 1.1.2 soft⁃ware.Western blot detected the expression of related proteins.RESULTS After CMEC was subjected to OGD/R treatment,ICA had a protec⁃tive effect at 10^(-1)60μmol·L^(-1);the results of the cell migration assay showed that each group of ICA could promote the migratory effect of CMEC(P<0.01,P<0.01);and the results of tube-for⁃mation assay showed that each group of ICA could significantly promote the generation of branches(P<0.01)and the capillary length exten⁃sion(P<0.05).Network pharmacology collected a total of 23 ICA action targets,1500 disease tar⁃gets and 12 key targets.GO function enrichment analysis found 85 results.KEGG pathway enrich⁃ment analysis found 53 results,involving AGERAGE signaling pathway,sphingolipid signaling pathway and VEGF signaling pathway.Molecu⁃lar docking results showed that ICA had better binding with core targets PRKCB,PRKCA and PTGS2.Western blot results showed that ICA could regulate the expression of PRKCB,PRKCA and PTGS2 proteins.The results of cell migra⁃tion assay,tube-formation assay and protein expression were reversed after addition of PKC inhibitor.CONCLUSION The potential mecha⁃nism of action of ICA against myocardial isch⁃emia-reperfusion injury may be related to the reg⁃ulation of processes such as CMEC migration and angiogenesis,and it functions through the key target gene PKC.
