Fungus Aspergillus sp., which was isolated from soft coral Sarcophyton tortuosum, was cultured in the GPY medium containing glucose 10 g/L, peptone 5 g/L, yeast extract 2 g/L, sea water 1 L, at pH=7.5. Four com pounds...Fungus Aspergillus sp., which was isolated from soft coral Sarcophyton tortuosum, was cultured in the GPY medium containing glucose 10 g/L, peptone 5 g/L, yeast extract 2 g/L, sea water 1 L, at pH=7.5. Four com pounds, 3,6-diisobutyl-2（1H）-pyrazinone（1）, 3-isobutyl-6-（1-hydroxy-2-methylpropyl）-2（1H）-pyrazinone（2）, 3-methoxy-4-methyl-2,4-dien-pentanoic acid（3） and penicillic acid（4） were obtained from the AcOEt extract of the culture broth. Their structures were elucidated mainly based on the NMR, HR-EI-MS and X-ray single crystal diffraction experimental data. Compound 3 is a new compound. Compound 1 was previously proposed to be the tautomer of flavacol（3,6-diisobutylpyrazin-2-ol, 5）. However, the evaluation of the NMR and X-ray single crystal diffraction experimental data permitted us to propose that compound 1 existed as amide form instead oftautomers. Compound 1 is stable both in solution and crystal due to strong intermolecular hydrogen bonds. Incidentally supplying anthracenone to the GPY medium with a dose of 100 mg/L, the yield of penicillic acid（4） in the culture broth of the strain Aspergillus sp. was increased dramatically from 6 mg/L to 57 mg/L.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.20502036 and 20602044)the Natural Science Founda-tion of Guangdong Province(No.05300667) the Fund for Innovative Chemical Experiment and Research of School of Chemi-stry and Chemical Engineering, Sun Yat-sen University, China
文摘Fungus Aspergillus sp., which was isolated from soft coral Sarcophyton tortuosum, was cultured in the GPY medium containing glucose 10 g/L, peptone 5 g/L, yeast extract 2 g/L, sea water 1 L, at pH=7.5. Four com pounds, 3,6-diisobutyl-2（1H）-pyrazinone（1）, 3-isobutyl-6-（1-hydroxy-2-methylpropyl）-2（1H）-pyrazinone（2）, 3-methoxy-4-methyl-2,4-dien-pentanoic acid（3） and penicillic acid（4） were obtained from the AcOEt extract of the culture broth. Their structures were elucidated mainly based on the NMR, HR-EI-MS and X-ray single crystal diffraction experimental data. Compound 3 is a new compound. Compound 1 was previously proposed to be the tautomer of flavacol（3,6-diisobutylpyrazin-2-ol, 5）. However, the evaluation of the NMR and X-ray single crystal diffraction experimental data permitted us to propose that compound 1 existed as amide form instead oftautomers. Compound 1 is stable both in solution and crystal due to strong intermolecular hydrogen bonds. Incidentally supplying anthracenone to the GPY medium with a dose of 100 mg/L, the yield of penicillic acid（4） in the culture broth of the strain Aspergillus sp. was increased dramatically from 6 mg/L to 57 mg/L.
